Download Technical Data Sheet

Version: 2
Revision Date: 07/08/2016
Purified anti-GABA(B)R2 Antibody (Previously Covance
catalog# MMS-5137)
Catalog# / Size
820501 / 100 µl
Clone
N81/2 (See other available formats)
Other Names
Gamma-aminobutyric acid type B receptor subunit 2,
gb2, GABA-BR2, GABA-B-R2, GABA-B receptor 2, GABA
-B receptor, R2 subunit, G protein-coupled receptor 51, G
-protein coupled receptor 51, gamma-aminobutyric acid
B receptor 2
Previously
Covance Catalog# MMS-5137
Isotype
Mouse IgG1
Ave. Rating
★★★★★ 0 reviews
Description
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter
in the mammalian brain where it acts at GABA-A receptors, which are ligand
-gated chloride channels. Chloride conductance of these channels can be
modulated by agents such as benzodiazepines that bind to the GABA-A
receptor. The GABA-A receptor is generally pentameric and there are five
types of subunits: alpha, beta, gamma, delta, and rho.
Western blot showing Clone N81/2 specificity.
Lane 1: Molecular Weight Standard; Lane 2:
Mouse Brain Membrane Lysate.
Staining of Clone N81/2 on formalin-fixed
paraffin-embedded mouse brain tissue.
Reactivity
Human, Mouse, Rat
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 862
-913 (cytoplasmic C-terminus) of human GABA(B)R2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the
storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You
can also check your vial or your CoA to find the most accurate storage condition for this
antibody.
Application
WB, IHC
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100*
• IHC: 1:100
Tissue: Formalin-fixed Paraffin-embedded
Pre-treatment: Citrate
Primary incubation: 24 hours at 2-8°C
Application Notes
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
*Expected MW: 105 kD
WB Positive Control: Mouse Brain Membrane Lysate
IHC Positive Control: Mouse Brain
RRID
AB_2564841 (BioLegend Cat. No. 820501)
Antigen Details
Gene ID
9568
UniProt
View information about GABA(B)R2 on UniProt.org
Documentation
Technical Data Sheet (pdf)
Certificate of Analysis
Safety Data Sheet
Related Protocols
Western Blotting Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
NeuroscienceProducts > Synaptic Biology > Postsynaptic Antibodies >
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