Ciliary neurotrophic factor may activate mature
... Bonni et al., 1997). This effect is likely related to the binding of the cytokine to its receptor, the three components of which are expressed by these precursors and immature cells (Bonni et al., 1997). In contrast, effects of CNTF on mature astrocytes have remained controversial. Astroglial activa ...
... Bonni et al., 1997). This effect is likely related to the binding of the cytokine to its receptor, the three components of which are expressed by these precursors and immature cells (Bonni et al., 1997). In contrast, effects of CNTF on mature astrocytes have remained controversial. Astroglial activa ...
Combining docking and molecular dynamic simulations in drug design
... expression, purification, and in vitro assay of hundreds if not thousands of proteins against libraries of hundred of thousands if not millions of compounds does not constitute a rational approach. The approaches and methodologies used in drug design have changed over time, exploiting and driving ne ...
... expression, purification, and in vitro assay of hundreds if not thousands of proteins against libraries of hundred of thousands if not millions of compounds does not constitute a rational approach. The approaches and methodologies used in drug design have changed over time, exploiting and driving ne ...
Computational Tools For Protein Modeling
... and peptide sciences, from the genome scale to the atomic level. As molecular biology is moving toward genome scale, a huge amount of biological data is being generated. Particularly, the Human Genome Project and other genome sequencing efforts are providing DNA sequences at a prodigious rate, and t ...
... and peptide sciences, from the genome scale to the atomic level. As molecular biology is moving toward genome scale, a huge amount of biological data is being generated. Particularly, the Human Genome Project and other genome sequencing efforts are providing DNA sequences at a prodigious rate, and t ...
Bile Acids and Bile Acid Sequestrants
... The small heterodimer partner (SHP) protein is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore tec ...
... The small heterodimer partner (SHP) protein is a member of the nuclear receptor family of intracellular transcription factors and is encoded by the NR0B2 (nuclear receptor subfamily 0, group B, member 2) gene. SHP is unusual for a nuclear receptor in that it lacks a DNA binding domain. Therefore tec ...
1055 BIOTECHNOLOGYDERIVED ARTICLES—PEPTIDE
... fragments, followed by separation and identification of the resultant fragments in a reproducible manner. It is a powerful test that is capable of identifying single amino acid changes resulting from events such as errors in the reading of complementary DNA (cDNA) sequences or point mutations. Pepti ...
... fragments, followed by separation and identification of the resultant fragments in a reproducible manner. It is a powerful test that is capable of identifying single amino acid changes resulting from events such as errors in the reading of complementary DNA (cDNA) sequences or point mutations. Pepti ...
Whole body and tissue protein synthesis in cattle
... method of Firschein & Shill (1966), and this was convcrted to collagen-N by multiplying by 1-42. Subtraction of collagen-N from total carcass N gave the amount of muscle N present. Cnlcirlufiort oj-rcsir/ts. The basic equation derived by Garlick rt (J/. ( I973) was used for all treatments as describ ...
... method of Firschein & Shill (1966), and this was convcrted to collagen-N by multiplying by 1-42. Subtraction of collagen-N from total carcass N gave the amount of muscle N present. Cnlcirlufiort oj-rcsir/ts. The basic equation derived by Garlick rt (J/. ( I973) was used for all treatments as describ ...
Relationship between Hot Spot Residues and Ligand Binding Hot
... sites in this region: two in the B1 pocket, one in the P1 pocket, and one in the B2 pocket (Figure 1a).16 The two sites in the B1 pocket are very close together, and hence, Figure 1a shows only three distinct sites, one in each of the three pockets. We used the FTMap server32 (http://ftmap.bu.edu) t ...
... sites in this region: two in the B1 pocket, one in the P1 pocket, and one in the B2 pocket (Figure 1a).16 The two sites in the B1 pocket are very close together, and hence, Figure 1a shows only three distinct sites, one in each of the three pockets. We used the FTMap server32 (http://ftmap.bu.edu) t ...
〈1055〉 biotechnology- derived articles—peptide mapping
... fragments in a reproducible manner. It is a powerful test that is capable of identifying single amino acid changes resulting from events such as errors in the reading of complementary DNA (cDNA) sequences or point mutations. Peptide mapping is a comparative procedure because the information obtained ...
... fragments in a reproducible manner. It is a powerful test that is capable of identifying single amino acid changes resulting from events such as errors in the reading of complementary DNA (cDNA) sequences or point mutations. Peptide mapping is a comparative procedure because the information obtained ...
Chapter 11 PowerPoint - Hillsborough Community College
... both CNS and PNS • ATP, the energy molecule, is now considered a neurotransmitter • Adenosine is a potent inhibitor in brain – Caffeine blocks adenosine receptors ...
... both CNS and PNS • ATP, the energy molecule, is now considered a neurotransmitter • Adenosine is a potent inhibitor in brain – Caffeine blocks adenosine receptors ...
Production of Polyclonal Antibodies against Sucrose Transporter
... The SDS – PAGE analysis was done with the concentration of 15% akrilamid for separating gel which contained 30% akrilamid, Tris – HCl pH 8.8, SDS 10%, 50 μL ammonium persulfate (APS) and 5 μL N, N, N', N'-tetramethylethylenediamine (TEMED) and the concentration of 4.5 % akrilamid for stacking gel 30 ...
... The SDS – PAGE analysis was done with the concentration of 15% akrilamid for separating gel which contained 30% akrilamid, Tris – HCl pH 8.8, SDS 10%, 50 μL ammonium persulfate (APS) and 5 μL N, N, N', N'-tetramethylethylenediamine (TEMED) and the concentration of 4.5 % akrilamid for stacking gel 30 ...
Protein kinase inhibition: natural and synthetic variations on a theme
... E206, D220 and R260, in single letter code for amino acids) are shown as balls. ATP is bound at the base of the active site cleft between the two lobes while the inhibitor peptide PKI (residues 5-241, shown in white, binds on the surface of the large lobe. (b) An expanded view of the small lobe wher ...
... E206, D220 and R260, in single letter code for amino acids) are shown as balls. ATP is bound at the base of the active site cleft between the two lobes while the inhibitor peptide PKI (residues 5-241, shown in white, binds on the surface of the large lobe. (b) An expanded view of the small lobe wher ...
Plasma Lipoproteins
... into ligands that can be recognized by SR-A receptors include oxidation of the lipid components and apolipoprotein B. Unlike the LDL receptor, the scavenger receptor is not down-regulated in response to increased intracellular cholesterol. Cholesteryl esters accumulate in macrophages and cause their ...
... into ligands that can be recognized by SR-A receptors include oxidation of the lipid components and apolipoprotein B. Unlike the LDL receptor, the scavenger receptor is not down-regulated in response to increased intracellular cholesterol. Cholesteryl esters accumulate in macrophages and cause their ...
Mapping allosteric connections from the receptor G proteins
... dissociation of the activated G␣(GTP␥S) subunit from G␥ and the receptor. Upon dissociation of G␣(GTP␥S) from the complex, the EPR spectra for all sites except for 50R1 reveal an increase in R1 mobility (Fig. 2C). A comparison of the resulting G␣i(GTP␥S) spectra (Fig. 2C, green traces) with those o ...
... dissociation of the activated G␣(GTP␥S) subunit from G␥ and the receptor. Upon dissociation of G␣(GTP␥S) from the complex, the EPR spectra for all sites except for 50R1 reveal an increase in R1 mobility (Fig. 2C). A comparison of the resulting G␣i(GTP␥S) spectra (Fig. 2C, green traces) with those o ...
Supplemental Methods
... magnetic bead-handling robot (KingFisher 96; Thermo Electron Corporation, Vantaa, ...
... magnetic bead-handling robot (KingFisher 96; Thermo Electron Corporation, Vantaa, ...
Powerpoint slides
... Motif (sequence context): conserved pattern of amino acids that is found in two or more proteins Motif (structural context): combination of several secondary structure elements (also referred to as super-secondary structures and folds) Fold: (also referred to folding motif) larger combination of sec ...
... Motif (sequence context): conserved pattern of amino acids that is found in two or more proteins Motif (structural context): combination of several secondary structure elements (also referred to as super-secondary structures and folds) Fold: (also referred to folding motif) larger combination of sec ...
Python Libraries for Computational Chemistry and Biology
... Proteins often come in families, derived from a common ancestor and separated by mutation and evolution. Knowing how molecules similar to VR1 work gives ideas of how VR1 works. Let’s search for those! But the techniques from (small molecule) chemical informatics don’t work well here. We need somethi ...
... Proteins often come in families, derived from a common ancestor and separated by mutation and evolution. Knowing how molecules similar to VR1 work gives ideas of how VR1 works. Let’s search for those! But the techniques from (small molecule) chemical informatics don’t work well here. We need somethi ...
INPS: predicting the impact of non-synonymous variations on protein
... 3.1 Computing the change of protein stability upon residue substitution For sake of clarity, we first evaluated to which extent each feature contributes information to the problem of computing changes in DG values upon residue substitution in the protein sequence (without using the inverse mutations ...
... 3.1 Computing the change of protein stability upon residue substitution For sake of clarity, we first evaluated to which extent each feature contributes information to the problem of computing changes in DG values upon residue substitution in the protein sequence (without using the inverse mutations ...
Releasable conjugation of polymers to proteins
... the first reported example of this strategy was reported by Woghiren et al. who modified the activesite cysteine residue of papain with a 4-pyridyl disulfide activated mPEG.28 When the activated mPEG was added drop-wise to papain during the conjugation step, the recovered product did not contain an ...
... the first reported example of this strategy was reported by Woghiren et al. who modified the activesite cysteine residue of papain with a 4-pyridyl disulfide activated mPEG.28 When the activated mPEG was added drop-wise to papain during the conjugation step, the recovered product did not contain an ...
poster PDF
... cells in the pancreas release insulin into the blood in response. This signal tells cells to take the sugar out of the blood for direct conversion into energy or storage as glycogen or fatty acids. Later, as blood sugar levels drop, another pancreatic hormone, glucagon, manages the release of sugar ...
... cells in the pancreas release insulin into the blood in response. This signal tells cells to take the sugar out of the blood for direct conversion into energy or storage as glycogen or fatty acids. Later, as blood sugar levels drop, another pancreatic hormone, glucagon, manages the release of sugar ...
protein - eLisa UGM
... 1. A 5-ml biuret reagent is mixed with a 1-ml portion of protein solution (1 to 10mg protein/ml). The reagent include copper sulfate, NaOH, and K-Na-tartrate, which is used to stabilize the cupric ion in the alkaline solution 2. After the reaction mix is allowed to stand at room temperature for 15 o ...
... 1. A 5-ml biuret reagent is mixed with a 1-ml portion of protein solution (1 to 10mg protein/ml). The reagent include copper sulfate, NaOH, and K-Na-tartrate, which is used to stabilize the cupric ion in the alkaline solution 2. After the reaction mix is allowed to stand at room temperature for 15 o ...
Protein A CIP Resin
... to eliminate protein A ligand contamination will not even be needed to meet FDA’s residue ligand standard when the concentration of ligand is only 1 to 3 ppm. ...
... to eliminate protein A ligand contamination will not even be needed to meet FDA’s residue ligand standard when the concentration of ligand is only 1 to 3 ppm. ...
Protein Denaturation Studies Using the Pyris 6 DSC
... yields a broad, less intense denaturation peak as compared to some other proteins or the same concentration. The level of sensitivity obtained from the Pyris 6 DSC is outstanding, as the full Y-axis (heat flow) scaling is only 120 uW. Additionally, the Pyris 6 DSC had the resolution and sensitivity ...
... yields a broad, less intense denaturation peak as compared to some other proteins or the same concentration. The level of sensitivity obtained from the Pyris 6 DSC is outstanding, as the full Y-axis (heat flow) scaling is only 120 uW. Additionally, the Pyris 6 DSC had the resolution and sensitivity ...
- BioTek Instruments
... In applications that explore protein expression profiling, the sample consists of many different proteins all being expressed at different amounts, each with varying abundance of the phenyl ring-containing amino acids. Therefore molar absorptivities cannot be known. Typically a calibration curve usi ...
... In applications that explore protein expression profiling, the sample consists of many different proteins all being expressed at different amounts, each with varying abundance of the phenyl ring-containing amino acids. Therefore molar absorptivities cannot be known. Typically a calibration curve usi ...
(Rev. 9/04), Biographical Sketch Format Page
... Tanis, J.E., Bellemer, A., Moresco, J.J., Forbush, B., and Koelle, M.R. (2009). The potassium chloride cotransporter KCC-2 coordinates development of inhibitory neurotransmission and synapse structure in C. elegans. J. Neurosci., in press. Selected invited reviews and book chapters Koelle, M.R. (199 ...
... Tanis, J.E., Bellemer, A., Moresco, J.J., Forbush, B., and Koelle, M.R. (2009). The potassium chloride cotransporter KCC-2 coordinates development of inhibitory neurotransmission and synapse structure in C. elegans. J. Neurosci., in press. Selected invited reviews and book chapters Koelle, M.R. (199 ...