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Product:
Synonyms:
Anti-CIN85, C-Terminal, Human
(Rabbit)
Cbl-Interacting Protein of 85 kDa
Cat. No.:
231006
Lot No.:
Size:
Storage:
Usage:
B50401
100 µl
−20°C
ELISA (0.1 mg/ml);
immunoblotting (1:500);
immunofluorescence (1:100);
immunoprecipitation 1-2 g
Product Specifications:
Source:
General Description:
Immunogen:
Formulation:
Preservative:
Specificity:
Special Instructions:
Potency/Assay Conditions:
Germany
Tel 0800 6931 000
Rabbit
Recognizes CIN85, a protein involved in the degradation of several tyrosine
kinase receptors including epidermal growth factor (EGF), hepatocyte
growth factor (HGF), platelet derived growth factor (PDGF), and c-Kit
receptors. Also known as Ruk (regulator of ubiquitous kinase) and SETA
(SH3 domain-containing gene expressed in tumorigenic astrocytes), CIN85
is an ubiquitously expressed adaptor protein with three SH3 domains. It
interacts with several proteins including Cbl, Cbl-b, Grb2, Crk, and
p130Cas. CIN85 is involved in endosomal sorting and degradation of
receptor tyrosine kinases. Following ligand binding, receptor tyrosine
kinases become phosphorylated, bind to, and phosphorylate Cbl or Cbl-b.
These proteins polyubiquitinate the phosphorylated receptor and then
recruit and monubiquitinate CIN85. CIN85 is constitutively associated with
endophilins which are involved in clathrin-coated pit formation and
subsequent endocytosis. This leads to the degradation of the activated
receptor. It has recently been reported that malignant cells seem to escape
the rapid mechanisms of down regulation despite their association with
Cbl/Cbl-b and CIN85.
Immunogen used was a synthetic peptide (KIRLRLQMEVNDIKKALQSK)
corresponding to amino acid residues 646 - 665 of CIN85.
Affinity purified polyclonal antibody supplied at 1 mg/ml in PBS, pH 7.4.
None
Detects the ~85 kDa CIN85 protein in human, mouse, porcine, and rat. Will
also detect the mono-ubiquinated form of CIN85.
Avoid freeze/thaw cycles.
Variables associated with assay conditions will dictate the proper working
dilution.
USA & Canada
Tel (800) 628-8470
United Kingdom
Tel 0115 9430 840
E-mail address for technical inquiries: [email protected]
Find our current product data sheets on the web: http://www.calbiochem.com
Revised: 21-Jan-03
CN:
References:
Haglund, K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12191.
Petrelli, A., et al. 2002. Nature 416, 187.
Souberyan, P., et al. 2002. Nature 416, 183.
Szymkiewicz, I., et al. 2002. J. Biol. Chem., in press.
For research use only, not for diagnostic use.