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SCIENCE STARTER #3 – 11/18/16 Find the other half of this DNA sequence: A-A-G-T-C-T-G-C-A-T-G-T-A There is 15% Adenine. Figure out the percentages of the other nucleotides. TAKE OUT YOUR DNA BOOKS NOTES 5.2 – DNA REPLICATION Chapter 10 pg. 200-202 REPLICATION Purpose of Replication - Making copies of DNA Location - Nucleus Enzymes used: Helicase and DNA Polymerase DNA REPLICATION STEP 1 – UNZIP DNA Helicase unzips DNA by breaking the hydrogen bonds between nucleotides Replication Fork: point where DNA strands separate. STEP 2 – ADD BASES DNA polymerase adds complementary nucleotides to the open areas Nucleotides attach from surrounding area STEP 3 - FINISH Process continues until entire strand is copied Result is two strands of DNA Semi-Conservative Replication - Each strand is 50% new and 50% old DNA ACCURACY AND REPAIR Copies must be 100% accurate Enzymes follow DNA polymerase, proofreading and fixing errors Mutation= change in nucleotide at one location in DNA Cancer: a mutation that has led to uncontrolled cell reproduction EXIT TICKET Eureka! You just found a way to figure out how much of each nucleotide there is AND how to replicate DNA! Briefly describe what the complementary base pairing and Chargaf f rules are. Briefly explain EACH step of DNA replication so the other scientists can learn from you