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#140 MIC# Lap No.#8#. Microorganisms The Grouping of Organisms into KINGDOMS is based on three factors: 1. Cell Type (prokyotic or eukaryotic). 2. Cell Number (unicellular or multicellular). 3. Feeding Type (autotroph or heterotroph). 1. Cell Type The presence or absence of cellular structures such as the nucleus, mitochondria, or a cell wall Prokaryotes or 1 Eukaryotes #140 MIC# Prokaryotes (Bacteria); DO NOT HAVE: • An organized nucleus. • One organelle. • Euokaryotes DO HAVE: • Nucleus organized with a membrane. • Other organelles. 2nd criteria for Kingdom Divisions: Cell Number • Unicellular: single celled organism (protozoans, bacteria, some algae). • Multicellular: many celled organism cells start to specialize/differentiate. 2 #140 MIC# 3rd Criteria for Kingdom Divisions: Feeding Type; Autotroph or Producer Heterotroph or Consumer Make their own food Must eat other organisms to survive(Includes decomposers – those that eat dead matter) There used to be only 5 kingdoms 1. Monera 2. Protista 3. Fungi 4. Plantae 5. Animalia ـــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ 3 #140 MIC# Bacteria ذاتية التغذية Autotrophic Bacteria Mutualism متكافلة Bactria غير ذاتية التغذية Heterotrophic Bacteria` (مترممةCommensalism متعايشه .)Saprophytic Parasitic متطفلة أو انتهازيهPathogenic(ممرضة .)opportunistic Bacterial Morphology Bacillus البكتيريا العصويه Coccus البكتيريا الكرويه Filamentous البكتيريا الخيطيه Spirillum البكتيريا المغزل Aggregation System مفردهMono مزدوجه- Diplo في سالسلStrepto في عناقيدStaphylo4 #140 MIC# 1- Coccus بكتيريا كروية موجبة لجرام من مستعمره لبكتيريا Staphylococcus aureus يُالحظ الشكل العنقودي لهذا الجنس. 2- Bacillus أنظمة التجمع المختلفة لخاليا البكتيريا العصوية Arrangement of bacilli 5 #140 MIC# صورة الكتروميكروسكوبيه لبكتيريا عصويه قصيرة Electromicrograph of Short rods Escherichia coli بكتيريا القولون Bacilli Gram-positive bacteria in chains Bacillus anthracis Anthrax 6 البكتيريا المسببة لمرض #140 MIC# 3- Spirillum البكتيريا المسببة الكوليرا Vibrio cholerae 4- Spirochete البكتيريا المسببة لمرض الزهري Syphilis Treponema pallidum 7 #140 MIC# 5- Filamentous (Actinomycetes) Branched bacilli e.g. Nocardia Motility of Bacteria مثل معظم البكتيريا الكرويه :بكتيريا غير متحركه :بكتيريا متحركه بواسطة Escherichia coli : مثلFlagella (flagellum sn.) األسواط.1 )Myxobacteria (مثلGliding االنزالق.2 Monotrichous وحيدة السوط-ALophotrichous طرفية األسواط-BAmphitrichous قطبية األسواط-CPeritrichous محيطية األسواط-D- 8 #140 MIC# Response to Staining تنقسم البكتيريا تبعا ً الستجابتها لصبغة جرام إلى: Gram-positive bacteria مثلStaphylococcus aureus :وتكتسب اللون البنفسجي Gram-negative bacteria مثلE. coli :وتكتسب اللون األحمرالى الوردي 9 #140 MIC# Cyanobacteria Cyanobacteria ‘known as’ (Blue Green) Algae - Cyano = Green Blue. - Bacteria = They are more closely related to Prokaryotic bacteria (No Nucleus) than eukaryotic algae. Microscopic organisms - Gets energy through Photosynthesis. - Cyanobacteria can be found in almost every conceivable environment, from oceans to fresh water to bare rock to soil. Blue Green Pigment - Phycocyanin Blue Green Color. - Chlorophyll Green Color . Forms Unicellular, Colonies and Filaments. 10 #140 MIC# Some cell types of blue green bacteria Akinetes Heteocysts • Cells with resistant spores. • Thick walled cell. • hollow looking. • Larger than vegetative cells. To Provides the anaerobic environment for N fixation. • Thick walled cells. For Fixing nitrogen. Note (Nitrogen Fixation) ONLY Cyanobacteria and Prokaryotic bacteria can FIX nitrogen 11 #140 MIC# The species Cyanobacteria Nostoc Anabena Oscillatoria Gloeocapsa 1-Nostoc • Form: coccoi cells forming filaments in a gelatinous sheath. Often with specialized cells called heterocysts and akinetes. 12 #140 MIC# 2-Anabaena • Similar to Nostoc, except large balls are not normally formed. • anbaena Cells more elongate, less rounded. 3-Oscillatoria Shape-filaments and covered by multi-layered mucilage, the mucilagenous sheath is longer than the filament 13 #140 MIC# 4-Gloeocapsa • Shape-may be unicellular or made up of small groups of cells grouped within mucilage envelopes. • The cells are oval-shaped and enclosed within mucilage. ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ Algae Nearly 75% of the world’s oxygen produced by algae. One of the major food source of marine ecosystems. Eukaryotic, photosynthetic (autotrophs). 14 #140 MIC# STRUCTURE Four types of algae • Unicellular. • Colonial. • Filamentous. • Multicellular. CLASSIFICATION OF ALGAE SEVEN PHYLUM BASED ON • Color. • Type of chlorophyll. • Food-storage substance. • Cell wall composition. 15 #140 MIC# 1-Chlorophyta: Green Algae “Green algae” • Mostly found in fresh water. • Contain chlorophylls a and b. • Store energy as starch (carbohydrate). • Cell walls made of cellulose. “Green algae” Chlamydomonas Spirogyra Volvox 16 #140 MIC# a) Chlamydomonas Motile and Unicellular algae. b) Spirogyra Filamentous 17 #140 MIC# c) Volvox Consists of many Chlamydomonas-like cells bound in a colony. Each cell has two flagella. 18 #140 MIC# Phaeophyta : Brown Algae Brown algae” • Primarily marine algae. • No unicellular or colonial forms. • Contain chlorophyll c and brown pigment (Fucoxanthin). • Range in size from microscopic to kelos over 50 meters long. A)Fucus Also called “rockweed”. 19 #140 MIC# Rhodophyta : Red Algae Red algae” • Contain chlorophyll d and red pigments (phycobilins) • Mainly multicellular. • Typically in warm marine water. ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ Chrysophyta “Diatoms” • Unicellular. • Contain chlorophylls a and c as well as xanthophyll pigments (goldenbrown). • Have hard cell wall made of silicon dioxide. 20 #140 MIC# Euglenophyta Unicellular. • Motile (two flagella). • Contain chlorophylls a and b. • Mainly freshwater algae. • Main cell wall component: protein (flexible). Euglena Unicellular Nora Al-kubaisi 21