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Name____________________________________ Course:_______________________ Problem Set #3: Cellular Respiration, Fermentation, and Photosynthesis Choose the best answer for the following questions, and then tell why the answer you choose is correct. 1. Glycolysis leads to the production of two: a. Lactic acid, NADH, ATP d. Pyruvate, NADH, ATP b. Pyruvate, NAD+, ATP e. FADH2, Pyruvate, NAD+ c. CO2 , Pyruvate, NAD+ ______________________________________________________________________________ ______________________________________________________________ 2. During metabolism WHICH reaction proceeds in the absence of oxygen? a. Glycolysis and ETS c. Glycolysis and TCA cycle b. Fermentation and the ETS d. Glycolysis and Fermentation ______________________________________________________________________________ ______________________________________________________________ 3. Which is true of the intermediate step of metabolism? a. CO2 and ATP are released during the process b. A multienzyme complex removes a carboxyl group, transfers electrons to NAD+, and attaches a coenzyme. c. NAD+ is rejuvenated so glycolysis can continue d. Lactate is produced to rejuvenate free NAD+ ______________________________________________________________________________ ______________________________________________________________ 4. Which is the most important function of Glycolysis? a. Production of NADH b. Modification of Glucose c. The production of ATP d. The production of CO2 ______________________________________________________________________________ ______________________________________________________________ 5. In the Chemiosmotic (oxidative phosphorylation) mechanism, a. ATP production results from a proton gradient established by the electron transport chain. b. The difference in pH between the intermembrane space and the cytosol drives the formation of ATP. c. The energy released by the reduction and subsequent oxidation of components of the electron transport chain is transferred as a phosphate to ADP. d. The production of water in the matrix by the reduction of oxygen leads to a net flow of water out of the mitochondrion. ______________________________________________________________________________ ______________________________________________________________ 6. The main function of the TCA cycle is accomplished between which stage? a. Oxaloactetate and citrate c. Citrate and Succinate b. Isocitrate and Succinate d. Succinyl CoA and Malate ______________________________________________________________________________ ______________________________________________________________ 7. Which enzyme of the TCA cycle has a dual role with the ETS and is located in the inner membrane of the mitochondrion: a. Pyruvate dehydrogenase c. Fructose dehydrogenase b. NADH Dehydrogenase d. Cytochrome oxidase c. Succinate Dehydrogenase ______________________________________________________________________________ ______________________________________________________________ 8. Fatty acids inter metabolism after they have been beta oxidized to form: a. Carbon dioxide c. Glucose b. Acetyl groups d. H2CO3 ______________________________________________________________________________ ______________________________________________________________ 9. The only usable ATP is produced here in the TCA cycle? a. Between Malate and Fumerate b. Between Succinate and Fumerate c. Between Succinyl Co-A and Succinate d. Between Succinate and Fumerate ______________________________________________________________________________ ______________________________________________________________ 10. What is the terminal electron acceptor during mitochondrial respiration? a. Water c. Hydrogen b. Oxygen d. NADH ______________________________________________________________________________ ______________________________________________________________ 11. As a result of glycolysis, pyruvate oxidation and the citric acid cycle, only a small portion of the energy of glucose has been converted to ATP. At this point, the majority of the usable energy is contained in: c. Acetyl coenzyme A d. Pyruvate c. NADH and FADH2 d. Water _________________________________________________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________ 12. ATPs are synthesized in glycolysis and the TCA cycle by: a. Oxidative Phosphorylation c. Photophosphorylation b. Substrate-level Phosphorylation d. Anabolism ______________________________________________________________________________ ______________________________________________________________ 13. A product derived from pyruvate crosses the inner mitochondrion membrane by which of the following methods? a. Diffusion c. Active Transport b. Facilitated transport d. All of the above ______________________________________________________________________________ ______________________________________________________________ 14. Suppose the hexokinase activity was destroyed in the cell. Which of the following will occur? a. The cell will continue to produce ATP through the ETS b. The cell will continue to produce ATP in the citric acid cycle c. The cell will continue to produce ATP through Fermentation d. The cell will stop producing ATP ______________________________________________________________________________ ______________________________________________________________ 15. Dinitrophenol was once used as a component of diet pills for weight loss however, users were dying due to its consumption because the drug cause the inner mitochondrion membrane to become highly permeable which prevented ATP synthesis. How? a. After coenzymes dropped off electrons, they diffused into the intermembrane space from the matrix b. Electrons diffused from the matrix to the intermembrane space c. Protons diffused from the intermembrane space to the matrix, by-passing ATPsynthase d. Pyruvate is not allowed to enter the matrix ______________________________________________________________________________ ______________________________________________________________ 16. Which of the following least support the contention that the mitochondrion is a descendant of prokaryotes? a. They have porins in their outer membranes b. They have a diameter of .2 microns c. They swim freely in the eukaryotic cells d. They have circular chromosomes and 80s ribosomes 17. The light reactions of photosynthesis occurs: a. The stroma c. The matrix b. The thylakoid membrane d. The stoma ______________________________________________________________________________ ______________________________________________________________ 18. Which molecule binds to carbon dioxide to form a six-carbon sugar in the Calvin Benson Cycle? a. Ribulose Bisphosphate c. Glyceraldehyde-3-phosphate b. Citrate d. Phosphoglyceraldehyde 19. The Calvin-Benson Cycle occurs: a. The stroma c. The matrix b. The thylakoid membrane d. The stoma ______________________________________________________________________________ ______________________________________________________________ 20. Which is not a product of Photosystem II? a. NADPH c. O2 b. ATP d. All are products of Photosystem II ______________________________________________________________________________ ______________________________________________________________ 21. CO2 required for carbon fixation, gets into the Calvin-Benson cycle through which structure of the leaf? a. The stroma c. The matrix b. The thylakoid membrane d. The stroma _________________________________________________________________________ ____________________________________________________________________ 22. How many pairs of electrons are removed in the TCA cycle? a. 4 b. 5 c. 6 d. 12 ______________________________________________________________________________ _____________________________________________________________ 23. The reason that anaerobes can produce a maximum of 38 ATPs from a molecule of glucose while aerobes can only produce 36 is because: a. Anaerobes do not lose two ATPs in glycolysis b. Anaerobes do not have an ETS c. Anaerobes do not undergo oxidative phosphorylation d. Anaerobes produces an extra FADH2 during the TCA cycle ______________________________________________________________________________ ______________________________________________________________ 24. During glycolysis, ATP are synthesized by: a. substrate-level phosphorylation b. oxidative phosphorylation c. photophosphorylation d. all of the above _________________________________________________________________________________________________________ ________ ______________________________________________________________________________ ______________________________________________________ 25. Cyanide is a poison that blocks the passage of electrons along the electron transport chain. Which of the following is a metabolic effect of this poison? a. The pH of the intermembrane space is much lower than normal b. Electrons are passed directly to oxygen, causing cells to explode. c. Alcohol would build up in the cells d. NADH supplies would be exhausted, and ATP synthesis would cease. e. No proton gradient would be produced, and ATP synthesis would cease. _________________________________________________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________ Study -The detail structure and function of the mitochondrion -The detail structure and function of the chloroplast I. Glycolysis Fermentation II. & III: Intermediate Step & Kreb Cycle (TCA Cycle) IV. Electron Transport System & Oxidative Phosphorylation Overview of Photosynthesis Photosystems I & II Calvin Benson Cycle