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What is homeostasis? “a stable, internal condition of a living organism” materials need to be brought into the cell and exported out of the cell to maintain homeostasis (a stable balance) How do you think Cell Size affects a cell’s ability to maintain homeostasis? Surface area to volume ratio of an object decreases, its size increases Small cell size makes it more efficient for the cell to regulate flow into and out of the cell Easier to maintain homeostasis Size of cells: a comparison What organelle is responsible for maintaining homeostasis? the cell membrane the cell membrane- up close! a dynamic structure The cell membrane has a bilipid layer. Where is that? Proteins Bilipid Bilayer Transport Protein Go to Section: Carbohydrate chains Phospholipids What part of the membrane do you think is attracted to water? Repelled by water? Polar heads love water. (Hydrophilic) Non-polar tails hide from water. (hydrophobic) What do you think the proteins do? Proteins Bilipid Bilayer Transport Protein Go to Section: Carbohydrate chains Phospholipids What do the surface markers do? (Carbohydrate chains and others?) What do they look like? Proteins Bilipid Bilayer Transport Protein Go to Section: Carbohydrate chains Phospholipids Cell Membrane – A fluid Mosaic Model What is the function of cell membrane? (Think about its structure) Acts as a barrier between cell and its environment Allows cell to maintain homeostasis Acts as selectively permeable membrane (determines what can enter/leave the cell) Cell-cell communication Surface markers – recognition (proteins, carbs and lipids) In your notes… Draw and label a cell membrane Discuss with your table partner how the structure of the cell membrane determines its function. You have 5 minutes to complete this task. the cell regulates movement in and out (through the membrane) by two main methods: NO expenditure of Energy uses concentration gradient • expenditure of Energy • does NOT use concentration gradient What is a concentration gradient??? Types of Cellular Transport Passive Transport cell doesn’t use energy HL concentration Weeee!!! high low Active Transport cell does use energy LH concentration This is gonna be hard work!! high low Passive Transport cell uses no energy molecules move randomly Molecules spread out from an area of HIGH to an area of LOW concentration until dynamic equilibrium is reached What is dynamic equilibrium? (HighLow) 3 Types of Passive Transport 1. Simple Diffusion 2. Facilitated Diffusion 3. Osmosis Have you heard the term diffusion before? What do you think it means? passive transport – simple diffusion small molecules pass through the lipid bilayer no energy is used substances move randomly, H L concentration results in even distribution of molecules molecules move randomly from an area where they are dense to a less dense area Solution eventually gets evenly distributed throughout the system. Most transport of materials into & out of cells occurs by diffusion • process is random, but NET movement is from HL concentration • upon reaching equilibrium, molecules still move equally back & forth across a membrane dynamic equilibrium diffusion leads to equal distribution of solutes (solute is component that is dissolved in the solution, ie sugar and water) *dynamic equilibrium (no future NET change in concentration) You now know what diffusion is, what do you think facilitated diffusion is? What does facilitated mean? passive transport - facilitated diffusion • no energy is required • substances cross the membrane with the help of carrier proteins • movement is from HIGH to LOW concentration and continues to equilibrium facilitated diffusion – carrier proteins Transport Proteins are specific – they “select” only certain molecules to cross the membrane Why do you think the cell membrane is referred as being selectively permeable? Cell membranes have protein channels Selectively permeable: Allows some molecules in and keeps other molecules out Pores The structure helps it be selective! Major membrane function: selective permeability Truth, Truth, Lie Write 2 truths regarding what we have learned about the cell membrane so far, write one lie – make it difficult! Teams of 4, keep score! Winner gets prize, but only if there is one winner, so make it difficult!! What is Osmosis? What does Osmosis have to do with diffusion?? Osmosis – passive transport of water diffusion of water through a selectively permeable membrane Water moves from HL concentration •Water moves freely through pores. •Solute (green) too large to move across. greater solute concentration NO net movement of water Lower solute concentration net flow of water Effects of Osmosis on Life Water is so small and there is so much of it the cell can’t control it’s movement through the cell membrane. Tonicity the measure of osmotic pressure (pressure created by concentration of water) of two solutions separated by a semi permeable membrane (such as a cell membrane!) hypotonic hypertonic isotonic What does hypo mean? Greek: Below, beneath Hypotonic Solution The solution outside the cell has a lower concentration of solutes What does that mean for the concentration of water inside the cell vs. outside the cell? Hypotonic Solution A higher concentration of water outside the cell than inside the cell. What do you think will happen to a cell when immersed in a hypotonic solution? Result: Water moves from the solution to inside the cell): cell swells and bursts open (cytolysis)! What does hyper mean? Greek: above, over Hypertonic Solution The solution outside the cell has a higher concentration of solutes What does that mean for the concentration of water inside the cell vs. outside the cell? Hypertonic Solution A lower concentration of water on the outside vs. the inside of the cell. What do you think will happen to a cell when immersed in a hypertonic solution? Result: Water moves from inside the cell into the solution: Cell shrinks (plasmolysis or crenation)! What does iso mean? Greek: equal, same Isotonic Solution Isotonic: The concentration of solutes in the solution outside the cell is equal to the concentration of solutes inside the cell. Result: Water moves equally in both directions and the cell remains same size! (Dynamic Equilibrium) isotonic solutions What type of solution are these cells in? A B C Hypertonic Isotonic Hypotonic Blood cell experiencing different tonicity Tonicity foldable You know what passive transport is…what are some traits of active transport? Why do you think it is called “Active” transport? active transport • Requires energy • Protein carriers are used to transport substances • Movement is from LH concentration • does not always result in even distribution of material Types of active transport 1. Ion Pumps Protein pumps Proton Pumps 2. CoTransport 3. Bulk Transport – Exocytosis/Endocytosis Protein Pumps transport proteins that require energy to do work •Example: Sodium / Potassium (Na+/K+)Pumps are important in nerve responses. Protein changes shape to move molecules: this requires energy! Sodium/Potassium Pump Sodium Potassium Pumps Proton (H+) Pump – Hydrogen ions pumped against their concentration gradient Cotransport The movement of two substances across the membrane, either in the same direction (symport), or opposite directions (antiport) Bulk Transport - Exocytosis Forces large material out of cell membrane surrounding the material fuses with cell membrane • Cell changes shape – requires energy Bulk Transport – Endocytosis: taking bulky material into the cell • • • Uses energy Cell membrane in-folds around food particle Three types: • • • Phagocytosis - solids Pinocytosis - liquids receptor mediated – certain kinds of molecules using specific receptors animation Graphic organizer what happens to a cell in solution is a function of the concentration of solutes in and out of the cell one of the most critical influences on a cell’s function is the medium (fluid) it is surrounded by Because the systems of living organisms function in a fluid environment… …the relative concentration of water and solutes must be maintained within fairly narrow limits How Organisms Deal with Osmotic Pressure Paramecium (protist) removing excess water video •Bacteria and plants have cell walls that prevent them from over-expanding. In plants the pressure exerted on the cell wall is called tugor pressure. •A protist like paramecium has contractile vacuoles that collect water flowing in and pump it out to prevent them from over-expanding. •Salt water fish pump salt out of their specialized gills so they do not dehydrate. •Animal cells are bathed in blood. Kidneys keep the blood isotonic by remove excess salt and water. OSMOREGULATION: The ability to regulate the osmotic concentration of internal body fluids and keep them constant despite fluctuations in their external environment. success = balancing H2O gain/loss so… animals have adaptations which allow them to osmoregulate based on the environment they live in special vesicles and vacuoles Helps maintain homeostasis for water balance (osmotic equilibrium) contractile vacuole central vacuole central vacuole (plants ONLY) Maintaining internal hydrostatic pressure or turgor within the cell contractile vacuole (animals ONLY) found in certain unicellular organisms pumps fluid from in the cell to the outside by alternately filling and then contracting