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Transcript
Microscopes • • • • • • • Mid 1600s 1665 Robert Hooke 1st Compound Microscope After looking at Cork Saw cambers called Cells Anton Van Leeuwenhoek Pond water (animalcules) Cork Cells / Microscopic Animals • Cork Cells at 100X Magnification / Plankton have limited powers of locomotion Scanning Electron Microscope • SEM is a type of electron microscope that images the samples surface by scanning it with a high-energy beam of electrons. Transmission Electron Microscope • TEM uses a beam of highly energetic electrons to examine objects very closely, on a fine scale. A TEM shines a beam of electrons through an object. Scanning Probe Microscope • SPM is a branch of microscopy that forms images of surfaces using a physical probe that scans the specimen. Microscope practice: Drawl the 3 specimens you see. For your first specimen call over the teacher so she can check your image Name of specimen: ___________________ Name of specimen: ___________________ Name of specimen: ___________________ Cell theory • All organisms are comprised of one cell or more. • Cell is the basic unit of life • All cells come from preexisting cells What is an organelle? Membrane bound structures with particular functions within eukaryotic cells Types of Organelles • • • • • • • • • • • Nucleus Cell membrane Ribosomes Endoplasmic reticulum Golgi Apparatus Lysosomes Vacuoles Mitochondria Chloroplast – Plants only Cell wall – plants only Cytoskeleton Bacteria cell Prokaryotic Cells Organelles: Have no nucleus- DNA is free floating Only have: cell Membrane Cell wall Ribosomes Cytoplasm Also have flagella or cilia to assist in moving Eukaryotic Cells Have multiple cells: Plants have: Cell wall Chloroplast And central Vacuole Animals: Have all other organelles that plants have Cell Wall • Plants: cell wall is rigid and made of fibrils of cellulose • Bacteria: cell wall is made up of polysaccharides and protein. Chloroplast • Captures light energy in plants and produces glucose in a process called photosynthesis. Click on chloroplast Ribosomes • Sites of protein synthesis • Scattered throughout cytoplasm and on rough ER • Comprised of protein and RNA molecules • Provide structural support for RNA during protein synthesis Click on ribosome Endoplasmic Reticulum - Rough ER – folded membrane covered in ribosomes – one of the sites of protein synthesis - Smooth ER – folded membrane with no ribosomes - lipid synthesis Golgi Bodies • Composed of six flattened membranous sacs • “Packages” and “delivers” proteins synthesized by ribosomes • Proteins arrive travel to the Golgi bodies in vesicles Golgi Bodies • They pass through one end and continue to pass over the sac until it forms glycoprotein which is a protein that has become chemically processed • When the altered glycoprotein reaches outermost layer, then bubble-like structures (vesicles) form and move through the cell membrane to the outside of the cell– exocytosis Click on golgi bodies Vacuole • Storage for the cell- All eukaryotic cells have but animal cells are much smaller than vaculoles in plant cells Mitochondria • Elongated fluid filled sacs • Move slowly through cytoplasm and reproduce by dividing • Has inner and outer membrane layers Mitochondria • Major site of ATP production – ATP are units of energy a cell can use to function (much like gasoline is to a car) Lysosome • “garbage men” of the cell • Membranous sacs • Powerful enzymes that breakdown nutrient molecules, foreign particles, or dead cell parts Microfilaments • Made of Tiny rods of actin protein that form meshwork through out the cells body • Provide cell mobility by enabling the cell to contract Click on picture Microtubules • Long slender tubes with diameter two to three times that of microfilaments. • Interwoven with Microfilaments • Composed of globular tubulin proteins Centrosome • Structure near Golgi Apparatus and nucleus • Consists of two hollow cylinders called centrioles • Lie at right angles and distribute chromosomes evenly to new cells during mitosis. Cilia • Motile extensions from certain cells • Tiny hairlike structures • Move to and fro, in succession, so that there is a wavelike motion Flagella • Motile extensions from certain cells • A cell will only normally have one flagellum • Swim motion, resembles a tail Cell Nucleus • Houses genetic material • Enclosed in a double layer nuclear envelope – inner and outer lipid bilayer membranes • Protein-lined channels called nuclear pores that allow for certain molecules to exit Nucleolus • • • • Small dense body in the middle of the nucleus composed largely of RNA and protein No surrounding membrane Forms in specialized regions of certain chromosomes • Ribosomes form in the nucleolus and move through nuclear pores to the cytoplasm Chromatin • Loosely coiled fibers of DNA and proteins = chromosomes • DNA = information for protein synthesis • Beginning of cell division – chromatin coil tightly and individual chromosomes become visible Cell Membrane Structure • A phospholipid consists of a – polar portion, called the head, – two longer fatty acids, called the tail. Cell Membrane Structure http://www.mhhe.com/biosci/esp/2002_general/Esp/default.htm When mixed with water, the heads are attracted to the polar water molecules. The nonpolar tails move as far from water as possible, and a double layer of phospholipids with tails to the interior results. Phospholipid bi-layer of a cell membrane Cell Analogy poster