Download Eukaryotic Cell

3/31/17
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Exercise 9
Introduction to the Eukaryotic Cell
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Announcements
n Students
have time to observe Week 8 plates
and finish their LNAs.
n Post
Lab 9/Pre Lab 10 are due by the time
your lab meets next.
n LNA Eukaryotic
Cell is due next week in lab.
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Goals
n  To
learn the basic structures associated with
eukaryotic cells.
n  To
identify the differences between animal and
plant cells.
n  To
understand the basis of cell culture, and the
changes imposed on cells under culture
conditions.
n  To
understand the techniques of fluorescence
and electron microscopy and cell culture.
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Animal Cell
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Plant Cell
The Exercise
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Part I: Plant cells: Onion epidermis
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Basic cell structure of a plant lacking chloroplasts
Iodine: observe nucleolus
n 
Janus Green: observe mitochondria
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The Exercise
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Part II: Animal cells: Human squamous epithelium
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n 
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Observe differences from the plant cells
Do the mitochondria vary in size from those found in
plant cells?
The Exercise
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Part III: Autofluorescence
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n 
View Tradescantia under the fluorescent microscope
Chlorophyll pigments autofluoresce
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The Exercise
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Part IV: Anacharis Leaf Cells
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View a living plant cell with chloroplasts
The Exercise
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Part V: Electron Micrographs and Cellular Infrastructure
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n 
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View EM transparencies
Identify organelles
You may want to take notes for future identification (i.e. Exam 2 and
Final)
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Be Able to Identify:
Nucleus
Nuclear envelope
Nuclear pore
Nucleolus
Rough Endoplasmic Reticulum
Ribosome
Mitochondria
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Be Able to Identify:
Golgi complex
top view
section view
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Golgi
top view
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Golgi
section view
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Be Able to
Identify:
Mitochondrion
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Be Able to
Identify:
Nucleus
Vacuole
Chloroplast
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Be Able to
Identify:
Thylakoid
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Be Able to
Identify:
Plasma
Membrane
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+ Summary of Exercise 9
n  Part
I: Onion epidermis
slide x 3: water / Iodine / Janus Green
n  size, nucleus, vacuole, cell wall/membrane, chloroplast,
mitochondria (?)
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n  Part
II: Cheek cells
slide x 2: water / Janus Green
n  size, nucleus, cell membrane, mitochondria (?)
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n  Part
III: Tradescantia
TA
n  Autofluorescence
n 
n  Part
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IV: Anacharis
Cytoplasmic streaming
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