Download Proteins relevant for Stem Cell Research - Bio

Stem Cell Research, Product flyer
Interest in any of the products,
request or order them at Bio-Connect.
Bio-Connect B.V.
Begonialaan 3a
6851 TE Huissen
The Netherlands
T NL +31 (0)26 326 44 50
F NL +31 (0)26 326 44 51
E [email protected]
W www.bio-connect.nl
T BE +32 (0)2 503 03 48
F BE +32 (0)2 503 03 27
Proteins relevant for
Stem Cell Research
Products currently
available from PeproTech:
Proteins Most Commonly Used in
Embryonic Stem Cell Media
FGF-basic
TGF-b1
Activin A
Other Proteins Used in Embryonic
Stem Cell Media
BMP-4
Noggin
sDKK-1
TGF-b3
Heregulinb
Wnt-3a
IGF-1
Vitronectin
GDF-3
ˇ
Proteins Commonly Used in Neural
Stem Cell Media
FGF-basic
EGF
Galectin-1
Wnt-3a
Proteins commonly used in Hematopoietic
Stem Cell Media
SCF
Flt3-Ligand
IL-3
TPO
IL-6
GM-CSF
G-CSF
Proteins used for Cell Reprogramming
Sox2
Sox2-TAT
Oct4*
Oct4-TAT*
Nanog
Nanog-TAT
Lin28*
Lin28-TAT
Klf4*
Klf4-TAT
cMyc*
cMyc-TAT*
* soon to be available
For a complete list of PeproTech’s product
line visit us at www.peprotech.com
Antibodies and ELISA Kits are available
for most of these products.
The human body develops from a single diploid cell called a
zygote and contains at adulthood an estimated 85 trillion cells,
of which more than 150 billion turnover everyday. All of these
cells originate from a tiny population of so-called “embryonic”
and “adult” stem cells which uniquely possess a long-term selfrenewal capacity and have the potential to differentiate into a
variety of cell lineages. Stem cell renewal and differentiation is
enabled by a battery of epigenetic regulatory factors and by various secreted cytokines.
PeproTech offers a number of regulatory factors and cytokines commonly used in stem
cell related research. These products are fully purified and, when possible, tested for
optimum performance in stem cell related applications.
Human Embryonic Stem Cells maintained in media using PeproTech's recombinant Human
Vitronectin as a chemically defined matrix component.
PHOTOS: Rick I. Cohen, Ph.D, Director, hESC Core Facility, Rutgers University, Stem Cell Research Center
Commonly used Cytokines in Stem Cell Research
Human Vitronectin
Synonyms: VTN, Serum-spreading factor, V75
Description: Vitronectin is a secreted glycoprotein which is synthesized in the liver. It
circulates primarily in monomeric form, but can undergo conformational change
to a structure that forms disulfide linked multimers. The multimeric Vitronectin
can efficiently bind to and incorporate into the extracellular matrix. Within
the matrix, Vitronectin can support cell adhesion through binding to various
integrins and other proteoglycans. Additionally, recombinant vitronectin can
function as a chemically defined matrix component in human embryonic stem
cell renewal media. Recombinant human Vitronectin is a 459 amino acid single
chain monomeric protein, which migrates at an apparent molecular weight of
75 kDa by SDS-PAGE under reducing conditions.
Continued
PeproTech • 5 Crescent Ave. • P.O. Box 275 • Rocky Hill, NJ 08553-0275 U.S.A.
Tel: (800) 436-9910 • (609) 497-0253 • Fax: (609) 497-0321 • [email protected] • [email protected] • www.peprotech.com
6/16/2011
For more information about PeproTech products visit www.peprotech.com
Commonly used Cytokines in Stem Cell Research Continued
Human Activin A
Synonyms: Inhibin beta-1, FRP, FSH-Releasing Protein
Description:Activin A is a TGF-β family member that exhibits a
wide range of biological activities including regulation
of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin
A in human colorectal tumors and in post-menopausal
woman have been implicated in colorectal and breast
cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, Follistatin. Human Activin A
is a 26.0 kDa disulfide-linked homodimer of two β A
chains, each containing 116 amino acid residues.
Human Noggin
Synonyms: none
Description: Noggin belongs to a group of diffusible proteins which
bind to ligands of the TGF-β family and regulate their
activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their
natural antagonists has major biological significance
during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin
was originally identified as a BMP-4 antagonist whose
action is critical for proper formation of the head and
other dorsal structures. Consequently, Noggin has
been shown to modulate the activities of other BMPs
including BMP-2,-7,-13, and -14. Targeted deletion of
Noggin in mice results in prenatal death and recessive
phenotype displaying a severely malformed skeletal
system. Conversely, transgenic mice over-expressing
Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and
severe osteoporosis. Recombinant human Noggin is
a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains.
Human Sox2
Synonyms: none
Description:Sox2, also known as sex determining region Y (SRY)-
box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high
mobility group (HMG) box. It plays an essential role
in maintaining the pluripotency of embryonic stem
cells (ESC) and determination of cell fate. Microarray
analysis showed that Sox2 regulates the expression of
multiple genes involved in embryonic development
including FGF-4, YES1 and ZFP206. Sox2 acts as a
transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA
sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2,
Oct4, cMyc, and Klf4, into human dermal fibroblasts
isolated from a skin biopsy of a healthy research fellow
was sufficient to confer a pluripotent state upon the
fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression,
and in the capacity to form teratomas in immune-deficient mice. Recombinant human Sox2 is a 34.3 kDa
protein containing 317 amino-acid residues.
Human TGF-b1
Synonyms: Transforming Growth Factor-beta1, Differentiation inhibiting factor, Cartilage-inducing factor
Description: The three mammalian isoforms of TGF-β, TGF-β1, β2,
β3, signal through the same receptor and elicit similar
biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of
the extracellular matrix. They are involved in various
physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted
predominantly as latent complexes which are stored
at the cell surface and in the extracellular matrix. The
release of biologically active TGF-β isoform from a
latent complex involves proteolytic processing of the
complex and /or induction of conformational changes
by proteins such as thrombospondin-1. TGF-β1 is the
most abundant isoform secreted by almost every cell
type. It was originally identified for its ability to induce
phenotypic transformation of fibroblasts and recently
it has been implicated in the formation of skin tumors.
Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a
single disulfide bond.
Murine Wnt-3a
Synonyms: Wingless-type MMTV (mouse mammary tumor virus)
integration site family member 3a
Description: Wnt-3a belongs to the Wnt family of signaling pro-
teins that play a key role in maintaining the integrity
of embryonic and adult tissues. Expression of Wnt-3a
occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS).
Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell
determination, cell proliferation, CNS development,
and cytoskeletal formation. Like other members of
this family, Wnt-3a contains a highly conserved lipid
modified cysteine rich domain that is essential for cell
signaling. During a biochemical process called the
canonical Wnt pathway; Wnt family members bind to
and activate seven-pass transmembrane receptors of
the Frizzled family ultimately leading to the disruption
of β-Catenin degradation. Intracellular accumulation
of β-Catenin increases translocation of the protein
into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt
signaling disrupts transcriptional activation of tumor
suppressor genes and has shown to result in neoplastic
transformation, oncogenesis, and human degenerative
diseases. Recombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues.
Due to glycosylation, the murine Wnt-3a migrates at
an apparent molecular weight of approximately 38.041.0 kDa by SDS-PAGE analysis under non-reducing
conditions.