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Stem Cell Research, Product flyer Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. Begonialaan 3a 6851 TE Huissen The Netherlands T NL +31 (0)26 326 44 50 F NL +31 (0)26 326 44 51 E [email protected] W www.bio-connect.nl T BE +32 (0)2 503 03 48 F BE +32 (0)2 503 03 27 Proteins relevant for Stem Cell Research Products currently available from PeproTech: Proteins Most Commonly Used in Embryonic Stem Cell Media FGF-basic TGF-b1 Activin A Other Proteins Used in Embryonic Stem Cell Media BMP-4 Noggin sDKK-1 TGF-b3 Heregulinb Wnt-3a IGF-1 Vitronectin GDF-3 ˇ Proteins Commonly Used in Neural Stem Cell Media FGF-basic EGF Galectin-1 Wnt-3a Proteins commonly used in Hematopoietic Stem Cell Media SCF Flt3-Ligand IL-3 TPO IL-6 GM-CSF G-CSF Proteins used for Cell Reprogramming Sox2 Sox2-TAT Oct4* Oct4-TAT* Nanog Nanog-TAT Lin28* Lin28-TAT Klf4* Klf4-TAT cMyc* cMyc-TAT* * soon to be available For a complete list of PeproTech’s product line visit us at www.peprotech.com Antibodies and ELISA Kits are available for most of these products. The human body develops from a single diploid cell called a zygote and contains at adulthood an estimated 85 trillion cells, of which more than 150 billion turnover everyday. All of these cells originate from a tiny population of so-called “embryonic” and “adult” stem cells which uniquely possess a long-term selfrenewal capacity and have the potential to differentiate into a variety of cell lineages. Stem cell renewal and differentiation is enabled by a battery of epigenetic regulatory factors and by various secreted cytokines. PeproTech offers a number of regulatory factors and cytokines commonly used in stem cell related research. These products are fully purified and, when possible, tested for optimum performance in stem cell related applications. Human Embryonic Stem Cells maintained in media using PeproTech's recombinant Human Vitronectin as a chemically defined matrix component. PHOTOS: Rick I. Cohen, Ph.D, Director, hESC Core Facility, Rutgers University, Stem Cell Research Center Commonly used Cytokines in Stem Cell Research Human Vitronectin Synonyms: VTN, Serum-spreading factor, V75 Description: Vitronectin is a secreted glycoprotein which is synthesized in the liver. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Additionally, recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media. Recombinant human Vitronectin is a 459 amino acid single chain monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. Continued PeproTech • 5 Crescent Ave. • P.O. Box 275 • Rocky Hill, NJ 08553-0275 U.S.A. Tel: (800) 436-9910 • (609) 497-0253 • Fax: (609) 497-0321 • [email protected] • [email protected] • www.peprotech.com 6/16/2011 For more information about PeproTech products visit www.peprotech.com Commonly used Cytokines in Stem Cell Research Continued Human Activin A Synonyms: Inhibin beta-1, FRP, FSH-Releasing Protein Description:Activin A is a TGF-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, Follistatin. Human Activin A is a 26.0 kDa disulfide-linked homodimer of two β A chains, each containing 116 amino acid residues. Human Noggin Synonyms: none Description: Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Human Sox2 Synonyms: none Description:Sox2, also known as sex determining region Y (SRY)- box 2, belongs to a diverse family of structurally-related transcription factors whose primary structure contains a 79-residue DNA-binding domain, called high mobility group (HMG) box. It plays an essential role in maintaining the pluripotency of embryonic stem cells (ESC) and determination of cell fate. Microarray analysis showed that Sox2 regulates the expression of multiple genes involved in embryonic development including FGF-4, YES1 and ZFP206. Sox2 acts as a transcriptional activator after forming a ternary complex with Oct3/4 and a conserved non-coding DNA sequence (CNS1) located approximately 2 kb upstream of the RAX promoter. The introduction of Sox2, Oct4, cMyc, and Klf4, into human dermal fibroblasts isolated from a skin biopsy of a healthy research fellow was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology, gene expression, and in the capacity to form teratomas in immune-deficient mice. Recombinant human Sox2 is a 34.3 kDa protein containing 317 amino-acid residues. Human TGF-b1 Synonyms: Transforming Growth Factor-beta1, Differentiation inhibiting factor, Cartilage-inducing factor Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond. Murine Wnt-3a Synonyms: Wingless-type MMTV (mouse mammary tumor virus) integration site family member 3a Description: Wnt-3a belongs to the Wnt family of signaling pro- teins that play a key role in maintaining the integrity of embryonic and adult tissues. Expression of Wnt-3a occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS). Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell determination, cell proliferation, CNS development, and cytoskeletal formation. Like other members of this family, Wnt-3a contains a highly conserved lipid modified cysteine rich domain that is essential for cell signaling. During a biochemical process called the canonical Wnt pathway; Wnt family members bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenin degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes and has shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Recombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues. Due to glycosylation, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.041.0 kDa by SDS-PAGE analysis under non-reducing conditions.