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All students, from all sections, welcome! The Biology 120 Peer Mentors and the University Learning Centre Present: Biol120 Mock Final Examination Friday, April 10th, 2015 Time: 1:00 – 1:50pm (50 minutes) Location: ARTS 146 Important note: This mock final exam was written by your Biology 120 Peer Mentors, not your professors! It is designed to help you test yourself on topics and concepts covered in class but should not be considered an exact preview of the final exam. You are required to remain in your seat for 30 minutes, but are free to leave the room after 30 minutes (1:30pm). No talking, please! No use of electronic devices (ie. cell phones, lap tops, etc.). This exam consists of 50 questions. Be sure to carefully read the questions and all possible answers. Choose the most correct answer. Only select one answer per question. You have 50 minutes to complete this exam. We encourage you to take note of your start and finish time, or to keep track of how many questions you were able to complete in the 50 minutes. This may help you to better judge your time for the final exam. Start Time: ________________ End Time: ________________ Questions completed: ___________ You are invited to return to the ART 146 ~2:00 for the Question and Answer period with your Peer Mentors. Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 1. Glycolysis occurs in: a) Mammals b) All eukaryotes c) Bacteria and archaea d) All of the above 2. The light independent reactions of photosynthesis occur in the: a) Thylakoid membrane b) Thylakoid lumen c) Stroma d) Lamella 3. The biggest difference between facultative anaerobes and strict anaerobes is: a) facultative anaerobes must have a steady oxygen supply, while strict aerobes require an oxygen-free environment. b) there is no difference, they are exactly the same. c) facultative anaerobes use a special kind of fermentation. d) facultative anaerobes can function with and without oxygen, while strict anaerobes can only function without oxygen. 4. In DNA replication, the new strand of DNA the ______________ grows ____________ away from the replication fork. a) Lagging strand, continuously b) Lagging strand, discontinuously c) Leading strand, continuously d) Leading strand, discontinuously 5. What are the outcomes of a missense mutation? a) Multiple amino acid alterations b) A single amino acid alteration c) A premature stop codon and the end of the polypeptide chain d) No change in the amino acid sequence at all 6. The kind of ATP synthesis that occurs in the Electron Transport Chain in Cellular Respiration is called: a) substrate-level phosphorylation b) fermentation c) photophosphorylation d) oxidative phosphorylation Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 7. In hamsters, brown fur (B) is dominant to white fur (b), and straight fur (S) is dominant to curly fur (s). If you buy a brown hamster with straight fur from the pet store, what kind of cross could you perform to determine whether or not your new pet is homozygous or heterozygous dominant? a) A di-hybrid cross with a homozygous dominant hamster b) A test cross with a curly haired, white hamster c) A test cross with another unknown dominant hamster d) A monohybrid cross with a black hamster 8. How are the light reactions of photosynthesis similar to aerobic respiration? a) Both processes synthesize NADPH b) Both processes start with a glucose molecule c) Both processes use a proton-motive force to generate ATP d) ATP synthesis is called photophosphorylation in both processes 9. A red sunflower (whose parents were both red) is crossed with a yellow sunflower (whose parents were both yellow) and each of the offspring have flowers that are a mix of red and yellow. No offspring express an orange colour. Which type of inheritance does this kind of sunflower show? a) Codominance b) Incomplete dominance c) Complete Dominance d) Epistasis 10. The RNA encoding a polypeptide that is transcribed from DNA is called _____, while the RNA that has the anticodon and carries a specific amino acid is ______, and ______ is the RNA component of ribosomes. a) transfer RNA; ribosomal RNA; messenger RNA b) messenger RNA; transfer RNA; ribosomal RNA c) messenger RNA; translation RNA; ribosomal RNA d) translation RNA; ribosomal RNA; messenger RNA 11. How many molecules of ATP can one molecule of NADH make in the Electron Transport Chain? a) none b) 1 molecule of ATP c) 3 molecules of ATP d) 38 molecules of ATP Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 12. In the Calvin Cycle three molecules of _____ are fixed to synthesize ______ molecules of G3P (a triose phosphate). a) ATP, one b) ATP, three c) CO2, one d) CO2, three 13. Which of the following is TRUE about DNA structure? a) It has a backbone with a slight positive charge. b) Each “rung” of the helix has either a pair of purines, or a pair pyrimidines hydrogen bonded together. c) The two strands of the helix are arranged in parallel. d) None of the above. 14. During oxidative phosphorylation, the Electron Transport Chain builds a proton gradient across_________ in eukaryotes. a) the cell membrane b) the inner mitochondrial membrane c) the outer mitochondrial membrane d) the nuclear membrane 15. During redox reactions, the molecule that donates an electron is said to be: a) Reduced b) Phosphorylated c) Oxidized d) Catabolized 16. Based on his experiments with pea plants, Mendel found he was able to predict that: a) Half of the offspring will have the same genotype as one of their parents b) As gametes are formed half the gametes carry one allele, and the other half carry the other allele c) The alleles of the genes that govern the two characters/traits segregate independently during the formation of gametes d) None of the above 17. How is ATP synthesized in photosynthesis? a) Rubisco b) A proton-motive force across the thylakoid membrane is used to synthesize ATP by chemiosmosis using the chloroplast ATP synthase c) A proton motive force is produced by NADH reduction and used by the ATP synthase d) Oxidative phosphorylation Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 18. What are the three phases, in order, of cellular respiration? a) Glycolysis, oxidative phosphorylation, pyruvate oxidation/citric acid cycle. b) Glycolysis, pyruvate oxidation/citric acid cycle, oxidative fermentation c) Glycolysis, pyruvate oxidation/citric acid cycle, oxidative phosphorylation d) Glycolysis, phosphate oxidation/citric acid cycle, oxidation phosphorylation 19. What type of inheritance is the following human pedigree (where a square represents a male, a circle represents a female, and any shaded shapes indicate an individual who HAS the trait) an example of? a) b) c) d) Autosomal dominance Sex-linkage on the X chromosome - recessive Autosomal recessive Sex-linkage on the Y chromosome - recessive 20. You are writing this mock final while breathing in oxygen and breathing out carbon dioxide. Which two sections of cellular respiration generates this CO2? a) Glycolysis and pyruvate oxidation b) Pyruvate oxidations and oxidative phosphorylation c) Glycolysis and the citric acid cycle d) Pyruvate oxidation and the citric acid cycle 21. What are the major products from photosynthesis that are used to perform carbon fixation during the Calvin Cycle? a) NADPH and ATP b) NADP+ and ATP c) NADP+ and ADP d) Electrons and Hydronium Ions Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 22. A nucleic acid was analyzed for its nucleotides and found to contain 12 percent A, 24 percent T, 24 Percent G, and 40 percent C. The nucleic acid must be which of the following? a) A double stranded DNA molecule b) A double stranded RNA molecule c) A single stranded DNA molecule d) A single stranded RNA molecule 23. Why is chlorophyll green? a) Because cholorphyll can only absorb the energy in a green photon, which allows it to emit a green colour. b) Because chlorophyll is unable to absorb the energy of a green photon, and all green light is reflected off the pigment instead. c) Because green is chlorophyll’s favourite colour. d) Because chlorophyll can only absorb the energy in blue, yellow, and green photons, which combine to be viewed as a green colour. 24. What occurs during glycolysis? a) Substrate level phosphorylation b) Reduction of fatty acids c) Oxidation of acetyl groups d) Oxidative phosphorylation 25. What can be the limiting factor of cell growth? a) Availability of micronutrients (Fe2+, Mg2+, etc.) b) Availability of carbon c) Availability of nitrogen d) All of the above 26. You cross two flies that are heterozygous for two genes and find that the phenotypic ratios of their offspring occur in the proportion 9:3:3:1. Does this mean they are independently assorted? a) Yes, they are definitely independently assorted b) No, they are definitely not independently assorted c) They may be independently sorted, but the genes may also be over 25mu apart on the chromosome d) They may be independently sorted, but the genes may also be over 50mu apart on the chromosome Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 27. Which one of the following occurs in the stroma of the chloroplast? a) Photophosphorylation b) Light reactions c) The Calvin cycle d) Electron transport chain 28. Which of the following sequences represent a correct order of enzymes required for DNA replication? a) Helicase, DNA polymerase, Primase, Ligase. b) Helicase, Ligase, DNA Polymerase, Primase. c) DNA Polymerase, Helicase, Primase, Ligase. d) Helicase, Primase, DNA Polymerase, Ligase. 29. Does all mRNA contain a start codon? a) No, because rRNA and tRNA do not code for proteins and therefore do not require start codons b) Yes, because mRNAs code for proteins and therefore require a start codon c) Yes, because rRNA and tRNA code for proteins and therefore require a start codon d) No, because mRNA does not code for protein and therefore does not need a start codon 30. Any diploid individual may only have how many alleles of each individual gene? a) 23 b) 2 c) 46 d) 12 31. Which of the following is NOT a common cellular component of both eukaryotic and prokaryotic cells? a) RNA b) Plasma membrane c) Ribosomes d) Mitochondria 32. Rubisco, Ribulose-1,5-bisphosphate carboxylase/oxygenase, is the.. a) Sugar of the Calvin cycle that catalyzes the fixation of CO2 into organic form b) Sugar of photosynthesis that catalyzes the fixation of CO2 into inorganic form c) Enzyme of the Calvin cycle that catalyzes the fixation of CO2 into organic form d) Enzyme of the Calvin cycle that catalyzes the fixation of CO2 into inorganic form Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 33. Glycolysis occurs in the __________, pyruvate oxidation occurs in the __________, and the citric acid cycle occurs in the __________________, respectively. a) Mitochondrion intermembrane compartment, mitochondrial outer membrane, cytosol. b) Cytosol, mitochondrial matrix, mitochondrial matrix. c) Mitochondrial matrix, mitochondrial matrix, cytosol. d) Cytosol, cytosol, mitochondrial matrix. 34. In Drosophila, two genes, one for body colour and one for eye colour, are carried on the same chromosome. The wild type grey body colour (G) is dominant to black body colour (g) and wild-type red eyes (R) are dominant to purple eyes (r). You make a cross between a fly with a grey body and red eyes and a fly with a black body and purple eyes. Among the offspring, about half have grey bodies and red eyes and half have black bodies and purple eyes. A small percentage have black bodies and red eyes or grey bodies and purple eyes. Which alleles are carried together on the chromosomes in each of the flies (parents) used in the cross? a) Gr & gR in the first parent and gr in the second b) GR in the first parent and gr in the second c) GR & gr in the first parent and gr in the second d) gr in both parents 35. Which of the differences between DNA and RNA are true? a) DNA uses cytosine, guanine, uracil, and adenine; whereas RNA uses cytosine, thymine, guanine, and adenine b) DNA has no free hydroxyl group on the 2’ carbon and RNA has a free hydroxyl group on the 2’ carbon c) DNA is usually single-stranded and RNA is usually double-stranded d) None of the above are true 36. The citric acid cycle yields: a) NAD+, FAD, ADP b) CO2, NADH, FADH2, ATP c) CO2, NADH, acetyl-CoA d) ATP 37. A recently replicated DNA daughter helix has a 5’ to 3’ strand with many fragments ending in disconnected adjacent sugar and phosphate groups. This cell likely has a problem with which enzyme? a) DNA Polymerase b) Primase c) Ligase d) Helicase Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 38. A major effect of the rise in cyanobacteria 2.5 billion years ago was… a) The accumulation of O2 in the atmosphere b) The widespread use of H2O as an electron donor for photosynthesis c) Formation of banded iron deposits d) All of the above 39. In which of the following is structure and function matched incorrectly? a) Thylakoids, light absorption by chlorophylls and carotenoids b) Thylakoids, Calvin cycle c) Thylakoids, electron transport d) Thylakoids, ATP synthesis by ATP synthase 40. What is one of the products of one turn in the Calvin cycle? a) NADH b) A three carbon sugar molecule (G3P) c) ATP d) Glucose 41. C. Botulinum is a rod-shaped bacterium that produces a toxin that causes botulism in humans. Most botulism cases occur when food that was improperly canned is eaten. These bacteria can only grow in the oxygen-free environment of a sealed can, and the moist food provides the bacteria with a ready energy source. How would C. botulinum be classified? a) As a facultative anaerobe b) As a obligate aerobe c) As a strict anaerobe d) As a photosynthetic aerobe 42. A chromosome is undergoing DNA replication. If the template strand has a sequence of 5’ – CGATTATCG – 3’, the enzyme primase will create a primer with which sequence (all sequences are written 3’ to 5’)? a) CGATTATCG b) CGAUUAUCG c) GCTAATAGC d) GCUAAUAGC 43. Sickle cell anemia is a disease in which a glutamate is substituted by a valine. What kind of mutation is this? a) missense b) deletion c) nonsense d) insertion Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 44. Which of the following statements describes a strand of eukaryotic mRNA? a) It must be spliced to cut out exons and seal together translatable introns b) It is transcribed by RNA polymerase prior to export from the nucleus, and translation by a ribosome c) It has a guanine cap on its 3’ end and a poly-A tail on its 5’ end d) It is a polymer of adenine, thymine, guanine, and cytosine bases 45. Where are electrons donated to from photosystem I? a) A complex located on the lumen face of the thylakoid membrane called NADP+ reductase where NADP+ is reduced to NADPH b) A complex located on the lumen face of the thylakoid membrane called NADP+ reductase where NADPH is reduced to NADP+ c) A complex located on the stroma face of the thylakoid membrane called NADP+ reductase where NADP+ is reduced to NADPH d) A complex located on the stroma face of the thylakoid membrane called NADP+ reductase where NADPH is reduced to NADP+ 46. In glycolysis, _________ is invested, but will pay off by producing _________ and _________ (forms of energy), respectively. a) 2 ADP, 4 ATP, 2 NADH b) 2 ATP, 4 ATP, 2 NAD+ c) 2 ATP, 4 ATP, 2 NADH d) 2 ADP, 4 ATP, 2 NAD+ 47. Colour-blindness is an X-linked genetic trait. If your dad was colour blind but your mom wasn’t colour blind or a carrier, what would be the probability of you being colour blind? a) 0% b) 50% c) 25% d) It depends on whether I am a boy or a girl 48. The result of epistasis is: a) Individuals with a variation of a disease from one extreme to another. b) When one genes influences multiple phenotypes in the offspring. c) Some expected phenotypes do not appear among offspring. d) When all expected phenotypes occur in a 1:1:1:1 ratio. Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, 49. The electromagnetic spectrum contains a wide range of electromagnetic radiation. However, in that range most of the radiation reaching the surface of the earth is contained in ultraviolet light, visible light and infrared radiation. Of visible light which of the following is true? a) Red light is more energetic and of a longer wavelength than blue light. b) Red light is less energetic and of a shorter wavelength than blue light. c) Red light is more energetic and of a shorter wavelength than blue light d) Red light is less energetic and of a longer wavelength than blue light 50. As the electrons flow through the Electron Transfer Chain, the electronegativity of the electron acceptors: a) increases, ensuring that the energy flows from high to low down the chain. b) increases, ensuring that the energy flows from low to high down the chain. c) decreases, ensuring that the energy flows from high to low down the chain. d) stays the same all the way down the chain. The end! Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss, ANSWERS: 1. d 2. c 3. d 4. b 5. b 6. d 7. b 8. c 9. a 10. b 11. c 12. c 13. d 14. b 15. c 16. c 17. b 18. c 19. c 20. d 21. a 22. c 23. b 24. a 25. d 26. d 27. c 28. d 29. b 30. b 31. d 32. c 33. b 34. c 35. b 36. b 37. c 38. d 39. b 40. b 41. c 42. d 43. a 44. b 45. c 46. c 47. a 48. c 49. d 50. a Questions and answers for this mock exam will be posted after today’s event http://www.usask.ca/ulc/sss,