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Cell Introduction • Prokaryotic Cell: A cell that is lacking a nucleus and most organelles • Eukaryotic Cell: A cell that contains a membrane bound nucleus and other membrane bound organelles. • Organelles: “mini-organ” part of the cell with a specific job. Prokaryotic Cells • A cell that lacks a nucleus • Still has Genetic information (DNA) just not contained in the nucleus • Bacteria and archaea are two types of prokaryotic cells • These appear to be the earliest organisms on earth. Eukaryotic Cells • A cell with a membrane bound nucleus and membrane bound organelles. • Genetic material is contained in the nucleus • These are broken up into Plant cells and Animal cells Two Basic types Cell membrane cytoplasm Prokaryote Cell membrane cytoplasm nucleus Eukaryote Cell organelles Compare and Contrast Prokaryotes Cell membrane Ribosomes Cell wall Eukaryotes Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton Animal cell -Eukaryote Plant cell - Eukaryote Cell Theory • The generalization that: • all living things are composed of cells • cells are the basic unit of structure and function in living things. • All cells come from existing cells Cell Organelles Organelle: “Mini” organ Nucleus • A large organelle that contains the cells genetic material (DNA) • The “brain” of the cell • Nuclear Envelope: a pair of membranes that surrounds the nucleus • Nuclear pores: Holes or pores in the nuclear envelope that allow materials to leave the nucleus. Nucleus Chromatin Two membranes of nuclear envelope Nucleolus Pore Rough endoplasmic reticulum Ribosomes Ribosome • A small organelle used to change DNA into proteins • The “assembly line” of the cell • Made up of proteins and nucleic acids • Ribosomes are found on the outside of the Endoplasmic Reticulum and others are found floating in the cell. Ribosome Smooth ER Rough ER Nuclear envelope Ribosomes Endoplasmic Reticulum An extensive network of membranes that builds cell materials Two types of Endoplasmic Reticulum Smooth ER Rough ER Nuclear envelope Ribosomes Endoplasmic Reticulum Rough E.R. Has ribosomes attached to the outside of the membrane Creates proteins Smooth E.R. Does Not have ribosomes attached to the membrane Creates enzymes and lipids Transport vesicle buds off Secretary (glyco-) protein inside transport vesicle Ribosome Sugar chain Glycoprotein Polypeptide Rough ER Golgi Apparatus An organelle made up of a series of membrane sacks Makes changes and directs the materials created by the E.R. Like a shipping store that packs, directs, and ships materials. “Receiving” side of Golgi apparatus Transport vesicle from ER New vesicle forming “Shipping” side of Golgi apparatus Transport vesicle from the Golgi Rough ER Transport vesicle (containing inactive hydrolytic enzymes) Plasma membrane Golgi apparatus Engulfment of particle Lysosome engulfing damaged organelle “Food” Lysosomes Food vacuole Digestion Vacuole A large membrane bound sack found inside of the cell Contractile Vacuole: Used to pump out excess water in cells Central Vacuole: Stores chemicals and helps plant growth. Nucleus Central vacuole Nucleus Contractile vacuoles LM 650 Colorized TEM 8,700 Chloroplast Lysosomes Membrane bound sack that contains digestive enzymes Lysosomes are used to break down macromolecules Breaks down incoming food Destroy harmful bacteria Recycle damaged organelles Chloroplast A photosynthetic organelle found in plant cells Converts sunlight and CO2 into sugar and oxygen Stroma Chloroplast Granum Intermembrane space TEM 9,750 Inner and outer membranes Mitochondria Mitochondrion The organelle that creates energy for cells Uses glucose to create chemical energy (ATP) Outer membrane Intermembrane space Found in plant and animal cells Creation of energy happens on the membranes inside of the mitochondria Inner membrane Cristae Matrix Cytoplasm The entire region between the nucleus and plasma membrane A fluid filled space Flagella and Cilia Flagella: a long, thin, whip-like structure Created with microtubules that allow a cell to move Cilia: smaller, and more numerous hair-like structures Created with bundles of microtubules Flagellum Electron micrographs of cross sections: TEM 206,500 Outer microtubule doublet Central microtubules Radial spoke Dynein arms Flagellum Basal body (structurally identical to centriole) TEM 206,500 Plasma membrane Basal body Plasma Membrane A thin outer layer of a cell Regulates the flow of molecules into and out of the cell Made up of a Phospholipid bilayer with membrane proteins, cholesterol, and carbohydrates embedded. Flagellum Electron micrographs of cross sections: TEM 206,500 Outer microtubule doublet Central microtubules Radial spoke Dynein arms Flagellum Basal body (structurally identical to centriole) TEM 206,500 Plasma membrane Basal body Cell Wall A strong layer outside of the plasma membrane, used to maintain shape in plant cells Cell wall is found only in certain bacteria and plant cells.