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Leader: Course: Instructor: Date: BIOL 212 Supplemental Instruction Iowa State University WORDS TO REMEMBER Biotechnology Plasmids Restriction Enzymes Gene cloning Conjugation Sticky ends Recombinant DNA Transgenics Lauren M Biol 212 Kukday Oct 3 Gene therapy DNA Ligase Vector DNA Tracing DNA REVIEW: Do we use introns or exons? Draw a strand of DNA that contains silencer, repressor, basal transcription factors, TATA box, (transcription factors): https://www.youtube.com/watch?v=ysxtZJUeTCE Why does these processes need to happen? Four advantages to use plasmids in gene cloning: 1. 2. 3. 4. Plasmids are self ____________. They are ___________________ for cell survival. They can ______________ into the bacterial genome They contain antibiotic _____________ genes. 1060 Hixson-Lied Student Success Center 515-294-6624 [email protected] http://www.si.iastate.edu Recipe for gene cloning: What do we need? Step 1: Step 2: Step 3: Step 4: What have we made? What can we use it for? Looking ahead: Gel Electrophoresis 1. You leave for the bathroom and coming back, you notice that someone has taken a bite from your cookie you left on your plate. You collect the saliva from the crime scene. You carry out PCR amplification and electrophorese the DNA. You suspect your mom, dad or your little brother (suspects 1, 2, and 3) to have taken a bite and collect hair samples from them and carry out the same methods. The results are shown below. Who is guilty of eating your cookie?