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Modern cell theory Modern biologists have made certain additions to the original cell theory which now states that 1. All organisms are made up of cells. 2. New cells are always produced from preexisting cells. 3. The cell is a structural and functional unit of all living things. 4. The cell contains hereditary information which is passed on from cell to cell during cell division. 5. All cells are basically the same in chemical composition and metabolic activities. As the cells of different parts of an organism (such as epithelial cell, muscle cell, nerve cell, etc). vary in shape, size and internal structure, no cell can be described as a typical one. In spite of all these variations, they are all cells, showing certain common fundamental properties. Plant cell Eukaryote v Prokaryote Eukaryote—Eu means true or real. This is what we mean when we talk about a cell. organelles Example Animal and plant cells Has Prokaryote- Pro means first or old or ancient cells. NO organelles Example Bacteria. Organelles – defined as little organs Examples of organelles: 1 nucleus 2 mitochondria 3 cytoplasm 4 cell membrane 5 vacuole 6 Chloroplast 7 Ribosomes 8 ER endoplasmic reticulum 2 types smooth, rough 9 Golgi, Golgi body, or Golgi complex, or the Golgi apparatus Types of Microscopes 3 types that we use today 1 Compound light uses 2 lens 2 TEM Transmission Electron Microscope invented in the 1950 Uses electrons for its energy source. Electrons are negatively charged particles that make bonds in chemical rxns. The specimen must be dead; it uses a vacuum for placing the specimen. The vacuum is to prevent air from disturbing the movement of the electrons. 3 SEM Scanning Electron Microscope Structure of cell Both prokaryotic and eukaryotic cells possess the basic features of a plasma membrane and cytoplasm. The plasma membrane is the outermost surface of the cell which separates the cell from the environment. The cytoplasm is the aqueous content within the plasma membrane. Plasma membrane : It is like any other membrane in the cell but it plays a very important function. It forms the border of a cell, so it is also called the cell membrane. It is primarily composed of proteins and phosphalipid. The phospholipids occur in two layers referred to as a bilayer. Protein is embedded within the lipid layer, or attached to the surface of it. The plasma membrane is elastic and very fluid because of protein and lipid. Normally the function of the plasma membrane is that of a gate-keeper. It allows certain important substances to enter and exit the cell. Cytoplasm and organelles : The cytoplasm is a semi-solid substance which is present in the cell and which gives structure, size, shape and foundation to the cell. It is enclosed by the plasma membrane. Within the cytoplasm are a number of microscopic bodies called organelles that perform various functions essential for the survival of the cell. Endoplasmic reticulum with the nucleus and the Golgi complex Endoplasmic reticulum (ER) : is one of the important organelles present in the cytoplasm. Endoplasmic reticulum is a series of membranes which extend throughout the cytoplasm in eukaryotic cells. In certain cases in ER there are submicroscopic bodies called ribosomes which are involved in production of protiens. Rough ER : In this kind of ER the ribosomes are presenton the surface.The endoplasmic reticulum is responsible for protein synthesis in a cell. Ribosomes are suborganelles in which the amino acids are actually bound together to form proteins. There are spaces within the folds of ER membrane are known as cisternae. Smooth ER : This type of ER does not have ribosomes. Another organelle is the Golgi body or Golgi apparatus (G.A.). The Golgi body is a series of flattened sacs usually curled at the edges. Proteins which have formed in ER are processed in G.A. After processing, the final product is discharged form the G.A. At this time the G.A. bulges and breaks away to form a dropline vesicle known as secretory vesicles. The vesicles move butward to the cell membrane and either insert their protien contents in the membrane, or release their contents outside the cell. There is another organelle which is related to the Golgi apparatus called the lysosome. The lysosome is derived from the Golgi body. It is a sac of enzymes in the cytoplasm, used for digestion within the cell. These enzymes break down particles of food taken into the cell and make the food product available for use. There are also cytoplasmic organelles called peroxisomes in the cell which produces the enzymes to degrade fat molecules. ]