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Webquest 16 DNA Name__________________________________________________________________ Part 2 – RNA, Transcription, Translation RNA Go to http://www.dnaftb.org/dnaftb/21/concept/index.html Read the text and answer the following questions 1. Where is RNA commonly found? ____________________________________________ 2. Describe what is meant by the “central dogma” in biology. _______________________________________________________________________ ____________________________________________________________________ 3. Name the 3 types of RNA and the general roles they play in the making of protein. *_____________________________________________________________________ *_____________________________________________________________________ *_____________________________________________________________________ Click on the animation button below. Step through the animation and compare and contrast the structure of RNA to DNA. Use the Venn diagram to compare and contrast. Then do a sketch of an RNA molecule (at least 10 nucleotides long using the all the appropriate bases at least twice). DNA RNA RNA Sketch (at least 10 nucleotides long with appropriate nitrogen bases) Transcription (DNA RNA) Go to http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf Answer the following questions as you move through the animation of Transcription Before clicking 1. The diagram represents what type of molecule? ______________________ Click once 2. What type of molecule is the RNA polymerase? _______________________ Click again 3. What function does the RNA polymerase have? _______________________ 4. Where in the cell do you think this is taking place? ____________________ 5. Explain how the mRNA molecule forms. _______________________________________________________________________ ____________________________________________________________________ Go to the DNAi website: http://www.dnai.org/a/index.html Click on “Copying the Code” at the bottom of the page, then click on “putting it together” at the top of the new page. Select “transcription”. Watch the animation 1. What does the blue molecule do? ______________________________________________________________________ 2. What is the yellow chain? ____________________ 3. What is T replaced with in RNA? _______________________ Translation (mRNA to protein) Go to http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/translation.swf Answer the following questions as you move through the animation of Translation Before clicking 1. The diagram represents what type of molecule? _____________________ Click once 2. Where in cell in this taking place? _______________________ Click again 3. What type of molecule is the tRNA (transfer RNA) bringing to the mRNA? _________________________________________________________ 4. Explain (in terms of nitrogen bases) how the tRNA docks on the mRNA ? _______________________________________________________________________ ____________________________________________________________________ Click until the end watching the process of translation 5. As the tRNAs dock on the mRNA bringing amino acids with them what type of molecule is created ___________________________________________________________________ Start the animation over 6. What are the 3 nitrogen bases on the tRNA carrying the amino acid “Met”? _________ 7. What are the 3 nitrogen bases on the mRNA that the “Met”-tRNA docks upon? _______________________________________________________ 8. Check out the next tRNA with its 3 nitrogen bases and see where it docks on the mRNA. Can you detect a pattern. If there are 20 amino acids then what is the minimum number of tRNAs that must exist. _______________________________________________________________________ ____________________________________________________________________ Go to the DNA website: http://www.dnai.org/a/index.html Click on “Reading the Code” at the bottom of the page, then click on “putting it together” at the top of the new page. Select “interactive”. 9. Practice translation using the computer animation, and write down the final amino acid sequence here: Part 3 Source http://www.lz95.net/lzhs/science/jhawkins/bioi/spring/ch12webquest.pdf Access the following site: http://gslc.genetics.utah.edu/ Click on “The Basics and Beyond” Click on “Tour of the Basics” and complete the online activity Click on “What is DNA?” (Begin & continue to click “Next”) 1. What is the DNA helix made up of? __________________________________________ _______________________________________________________________________ 2 DNA is made up of letters-words-sentences; what is the function of the sentences? _______________________________________________________________________ _______________________________________________________________________ Click on “What is a gene?” (Begin & continue to click “Next”) 3. What is a gene? _________________________________________________________ 4. Give 2 examples produced by genes? _______________________________________________________________________ _______________________________________________________________________ 5. What results when the instructions from a gene are changed? _______________________________________________________________________ _______________________________________________________________________ Click on “What is a chromosome?” (Begin & continue to click “Next”) 6 Chromosomes are made up of 2 things? _______________________________________________________________________ _______________________________________________________________________ 7. How many chromosomes do mosquitoes have?____________________________________ Click on “What is a protein?” (Begin & continue to click “Next”) 8. What is a protein?________________________________________________________ _______________________________________________________________________ 9. How is an individual protein made?___________________________________________ _______________________________________________________________________ 10. Where are the proteins assembled?_________________________________________ Click on “What is heredity?” (Begin & continue to click “Next”) 11. Where do your traits come from?____________________________________________ 12. What is a zygote?_________________________________________________ Click on “What is a trait?” (Begin & continue to click “Next”) 13. Give 2 examples of different types of traits? _______________________________________________________________________ _______________________________________________________________________ 14. How are traits defined?__________________________________________________ 16. What is a complex trait?_________________________________________________ Click on the Back and Return to the Basics and Beyond, Click on “Discover How Proteins Function” 17. How are proteins shaped? _______________________________________________________________________ 18. Give 2 examples of how scientists determine the function of proteins _______________________________________________________________________ _______________________________________________________________________ 19. What is a motif?________________________________________________________ Access the following site: http://www.dnaftb.org/dnaftb/ Under the heading “Molecules of Genetics,” Click on topic #15 Read the information provided Click on “Animation” (at the bottom) for the lesson (Begin reading & continue to click Next) 20. Who is Fredrick Miescher?_______________________________________________ 21. What are 2 nucleic acids ________________________________________________ and where are they found?__________________________________________________ 22 What did Phoebus Levene study?____________________________________________