Download Name Date__________________ DNA and Protein Synthesis

Name ______________________________________
Date__________________
DNA and Protein Synthesis Internet Activities
1.
Type in the address http://learn.genetics.utah.edu/units/basics/transcribe/
a. Click on the button Click here to begin.
b. Follow the instructions on the left hand side of the page to transcribe and translate the molecule.
c. Transcription
Write in the bases to make the mRNA molecule in the activity.
d.
DNAATTACGATCTGCACAAGATCCT
mRNATranslation
Write the amino acid sequence abbreviations that makes up the protein.
____________ ____________ ____________ ______________
____________ _______________
2.
Type in the address http://learn.genetics.utah.edu/units/biotech/gel/
Fill in the blanks as you progress through the tutorial on gel electrophoresis
a. __________________ _________________________ is a way to sort and measure DNA strands
b. Gel electrophoresis sorts DNA molecules according to ______________________.
c. The _________________ is the filter that sorts DNA strands.
d. __________________ samples are placed into holes at one end of the gel.
e. DNA strands are pushed through the gel by adding _______________ _________________.
f. Short strands move through the gel _________________ than long strands so shorter strands move
_____________________ away.
g. _______________ the DNA strands make them visible creating _______________ in the gel.
h. ____________________, ___________________, a flask, and a microwave are needed to make a gel.
i. The gel is melted in a ______________________ and then poured into a _______________. A _______________ is
placed in the mold to create holes or wells.
j. ______________ is placed in the gel electrophoresis box.
k. DNA samples are loaded into the well using a ________________________.
l. The _______________________ cord (negative) must be placed closest to the wells to run electricity through it.
m. Finally, the gel is _________________ so the band in the gel become visible.
n. What is the estimated size of the 3 three bands of the DNA sample on the gel?
Band 1 __________________ Band 2 __________________ Band 3 ___________________
3.
Go to http://nhscience.lonestar.edu/biol/biolab/index.htm
Go to Lab 16
Paternity Testing by Sumanas, Inc.
Watch the animation for paternity testing
Which bands did the child have in common with the mother? Number _______, ________, and _______.
Which bands did the child have in common with the father? Number ______, ________, and _______.
Which lane must contain the DNA for the father of the child? ___________
4.
Go to http://glencoe.com Fill in state- Georgia, click on student, and science in the drop down menu. Then click on enter.
From there, click on biology, and then select the dynamics of life edition for 2002. Go to Chapter resources, Unit 4, quiz.
Write the LETTER of the correct answer below
1.
___________
6. ___________
11. ___________
2.
___________
7. ___________
12. ___________
3.
___________
8. ___________
13. ___________
4.
___________
9. ___________
14. ___________
5.
___________
10. __________
15. ___________
5. Type in the following address in the menu bar http://www.zerobio.com/drag_oa/protein/overview.htm
You will be completing the transcription and translation activity by dragging and dropping the correct bases to practice the process of
protein synthesis.
TRANSCRIPTION
 Click on the transcription tab
 Read the introductory information on this process
 WHAT DOES THE SEQUENCE OF DNA THAT YOU ARE ABOUT TO TRANSCRIBE REPRESENT?

Complete the transcription of the DNA sequence that is given to you


List the m-RNA strand that you transcribed from the DNA sequence
Answer the questions below about your results and check your answers. Write your answers below
QUESTIONS
Which base in RNA is replaced by uracil?
How many mRNA codons are illustrated above?
What is the name of the enzyme that creates the mRNA copy from DNA?
What is the name of the sugar in the mRNA nucleotides?
What is the mRNA transcript for the DNA sequence, TTACGC
 Click Next on the lower right hand corner
TRANSLATION
 Read the information on translation to review this process
 Complete the translation of the m-RNA sequence by dragging and dropping the correct bases in order
QUESTIONS
 What organelle assists tRNA in translating the mRNA in the cytoplasm?
 The role of tRNA is to carry a(n):
 Is a tRNA anticodon more similar to DNA or RNA in nucleotide sequence?
 If the mRNA codon is CGA, the tRNA anticodon that binds with it is:
 Click Next on the lower right hand corner
 Protein Synthesis 1 Activity: Complete the activity and write your responses in the template below
QUESTIONS
1-How many amino acids are coded for by the DNA?
2-What protein does this DNA code for?
3-If instead of ACT, the first DNA triplet was ACG, which amino acid would be coded for?
4-What amino acid is carried by a tRNA with the anticodon, GUA?
5-Sickle cell anemia is a disease of red blood cells in which a genetic mutation in DNA leads to a mutation in hemoglobin. A single
base change alters the DNA sequence CTC to CAC which codes for the wrong amino acid. What amino acid is coded for by the
normal DNA sequence, CTC?
6-For the question above, what amino acid is coded for by the mutated DNA sequence, CAC?
Click Next on the lower right hand corner to take the interactive quiz and write the correct answers below
1.Transcription is the first step of Protein Synthesis and it occurs in the:
2. Translation is the second step of Protein Synthesis and it occurs in the:
3. If a DNA sequence consists of 12 nucleotides, how many mRNA codons will there be?
4. The enzyme that creates mRNA from a DNA sequence is called:
5. Each codon of mRNA (hence each triplet in DNA) codes for one:
6. The specific amino acid carried by a tRNA is determined it's:
7. True or False? When amino acids are brought in by tRNA, they are joined together by hydrolysis reactions to form the growing
protein.
8. True or False? Whenever a cell needs to, it can unzip DNA and make a transcript of a sequence so that a protein can be made. DNA
then zips up.
9. True or False? The same amino acid can be carried by different tRNA'S.
10. Although not discussed in this activity, Translation actually takes place in 3 steps:
6.
Go to the site http://www.quia.com/jg/65756.html
Play the matching game, concentration, flashcards, and word search. Once you have completed the activity, print the last page.
Any of the four activities completed will count as extra credit.