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Student Name: ________________________________ DNA Structure, Replication and Protein Synthesis Q1 (a) Insert the most appropriate words in each of the following spaces: A section of DNA that causes the production of a protein is called a ___________________. Sections of DNA that do not code for a particular protein are called _____________________________. The protein _______________________________ is responsible for holding the DNA in its highly coiled state. DNA is made up of units called _______________________________. A sequence of three bases is referred to as a ____________________________. (b) The diagram shows a representation of a section of a DNA molecule. What is represented by each of the letters A, C, D, G, P and T? A __________________________ C __________________________ D __________________________ G __________________________ P __________________________ T __________________________ (c) What type of bonding exists between the bases? __________________________________ Identify the two purine bases and the two pyramidine bases. Purine bases ________________________________________________________________ Pyramidine bases ____________________________________________________________ What name is given to the shape of the DNA molecule? _____________________________ (d) State three differences between DNA and RNA. 1 __________________________________________________________________________ 2 __________________________________________________________________________ 3 __________________________________________________________________________ (e) Write down the sequence of RNA bases that is complementary to the following DNA sequences TATCGGCA _________________________ ATGCATA _______________________________ 20(2) marks 1 Student Name: ________________________________ Q2 Give a reason for each of the following steps when isolating DNA from a plant tissue. Adding salt ___________________________________________________________________ _____________________________________________________________________________ Cooling the mixture after exactly 15 minutes ________________________________________ _____________________________________________________________________________ Filtering the mixture after blending ________________________________________________ _____________________________________________________________________________ Adding protease enzyme ________________________________________________________ _____________________________________________________________________________ Adding cold ethanol ____________________________________________________________ _____________________________________________________________________________ 5(3) marks Q3 What are the three main stages of protein synthesis called? 1 _______________________ 2 __________________________ 3 _______________________ Outline a use for each of the following in proteins synthesis: RNA Polymerase ______________________________________________________________ tRNA ______________________________________________________________ mRNA ______________________________________________________________ rRNA ______________________________________________________________ Start codon ______________________________________________________________ Anti-codon ______________________________________________________________ Amino acid ______________________________________________________________ 10(3) marks Q4 During what stage of the cell cycle does DNA replication take place? _______________________ Why is it necessary for DNA to make copies of itself? ____________________________________ _______________________________________________________________________________ Name the part of a double stranded chromosome to which spindle fibres attach during cell division _______________________________________________________________________________ Outline the process of DNA replication in four stages 1 ______________________________________________________________________________ 2 ______________________________________________________________________________ 3 ______________________________________________________________________________ 4 ______________________________________________________________________________ 7(2) marks 2