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Supplementary Figure 2 4 B FLK ** WT RNAi4 RNAi5 3 2 1 0 untreated cis-CA trans-CA Relative Expression Level Relative Expression Level A 2 FLC ** WT RNAi4 RNAi5 1 0 untreated cis-CA trans-CA Supplementary FIG. 2. Quantitative RT-PCR analysis of expression levels of flowering genes in the ZCE1 RNAi lines. Quantitative RT-PCR analysis shows the transcript levels of FLK (A) and FLC (B) upon cis-CA and trans-CA treatment, respectively. FLK is a positive regulator of flowering via suppressing the expression of another flowering gene FLC, while the role of FLC is to repress flowering. (MiHye Lim et al 2004, Scott D. Michaels and Richard M. Amasino 1999). Actin (ACT2) is used as the internal control, so each tissue’s transcription level was normalized against actin. WT untreated group was set to 1.0 for comparison. In the untreated groups, each kind of untreated tissue was compared with that of WT. In the cis-CA and trans-CA-treated groups, WT and two ZCE1 RNAi lines were compared to each untreated tissue. ** means 2 or more folds compared with untreated group. The qPCR condition was set at 95 C for 2 min; 60 cycles of (95 C, 3 sec; 59 C 30 sec); 60-95 C. Primers used for qPCR are listed as the following: ACT2 (At3g18780)-real time-F: 5’-GAC CAG CTC TTC CAT CGA GAA-3’ ACT2 (At3g18780)-real time-R: 5’-TCT CGT GGA TTC CAG CAG C -3’ FLK (At3g04610) -real time-F: 5’-CAA AAG CCA ACT CGC CAA AT-3’ FLK (At3g04610) -real time-R: 5’-TGA CCA TAG CCT CCT CCA CC-3’ FLC (At5g10140) -real time-F: 5’-AAC GTC GCA ACG GTC TCA TC-3’ FLC (At5g10140) -real time-R: 5’-CGA TCA AGG ATC TTG ACC AGG-3’