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Ubiquitin-dependent protein degradation (35, 8)
*
Transport proteins (112, 44)
*
Cell stress (44, 28)
*
Carbohydrate metabolism (70, 27)
*
*
Translation (134, 72)
*
Amino acid metabolism (49, 29)
*
Signal transduction (25, 8)
*
Lipid/Fatty acid/sterol metabolism (38, 23)
*
Functional classes
Progression of cell cycle (42, 15)
Oxidative phosphorylation (54, 22)
Transcription (87, 40)
Cell wall and maintenance (55, 28)
Nucleotide metabolism (20, 10)
Cytoskeletal proteins (9, 3)
Allergens (6,3)
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
P value
Supplementary data Figure 1
Hypergeometric probability analysis to study the functional categories
over-represented in A. fumigatus on exposure to artemisinin using microarray
data
Foot note:
The bar diagram shows percentage of differentially expressed genes in various functional classes of A. fumigatus treated with
artemisinin.
The functional classes such as ubiquitin-dependent protein degradation, transport proteins, cell stress, carbohydrate metabolism,
translation, progression of cell cycle, amino acid metabolism, lipid/fatty acid/sterol metabolism and signal transduction showing
p-value <0.05, marked with star (*) were considered to be significantly over-represented after hypergeometric probability
analysis (www.stattrek.com/tables/hypergeometric.aspx).
The values in bracket show number of genes expressed in solvent control treated A. fumigatus followed by number of
differentially expressed genes in A. fumigatus treated with artemisinin belonging to specific functional class. The total number
of genes expressed in solvent control treated A. fumigatus was 1542 [n=1138+404 (hypothetical proteins)] and differentially
expressed genes in A. fumigatus treated with artemisinin was 797 [n=553+192 (hypothetical proteins)]. Hypergeometric
probability was calculated with all the genes other than hypothetical proteins.