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Cell Structure and Function Mrs. MacWilliams Academic Biology Levels of Organization Atoms molecules macromolecules organelles cells tissues organs organ systems organism/species populations community ecosystem biome biosphere I. DISCOVERY OF THE CELL 1665 (England) Robert Hooke- used compound microscope to look at cork Looked like “cells”. 1673 (Holland) Anton van Leeuwenhoek look at pond water and samples from a human mouth discovered bacteria A. Cell Theory 1. All living things are made up of cells. 2. Cells are the basic units of structure and function in living things. 3. All cells are produced from existing cells. B. Prokaryotes and Eukaryotes 1. Smallest cell Mycoplasma bacteria are only 0.2 micrometers 2. Largest cell Ostrich egg 3. All cells contain the molecule that carries biological information—DNA. 4. All cells are surrounded by a thin, flexible barrier called a cell membrane 5. Cells fall into two broad categories, depending on if they contain a nucleus. C. Form follows function 1. Examples: a. Skin cells: flat, tightly packed because they protect/cover b. Nerve cells: long & stringy because they transmit impulses c. Red blood cells (RBCs): small & doughnut shaped because they carry O2 D. Prokaryotes 1. cells that do not enclose DNA in a nucleus 2. Generally smaller and simpler than eukaryotic cells 3. Bacteria are prokaryotes E. Eukaryotes are cells that DO enclose their DNA in a nucleus!!!! 1. Generally larger and more complex than prokaryotic cells 2. Eukaryotic cells are highly specialized 3. Plants, animals, fungi, and organisms commonly called “protists” II. Cell Structure A. Cell Organization 1. Cellular structures act as if they are specialized organs known as organelles, literally “little organs.” 2. Cytoplasm - gel-like fluid surrounded by plasma membrane, where organelles are found 3. Eukaryotic cells are much like a living version of a modern factory with specialized machines and assembly lines = different organelles 4. Cells, like factories, follow instructions and produce products with the main product being PROTEINS!!!! B. Nucleus- control center of the cell 1. Contains nearly all the cell’s DNA and the instructions for making proteins 2. Surrounded by a nuclear envelope composed of two membranes. 3. Nuclear envelope has thousands of pores, which allow material to move into and out of the nucleus. 4. Chromosomes contain genetic information that is passed from one generation of cells to the next. 5. Chromosomes are spread throughout the nucleus in the form of chromatin—a complex of DNA bound to proteins. 6. Most nuclei also contain a small, dense region known as the nucleolus. 7. The nucleolus is where the assembly of ribosomes begins. Organelles That Store, Clean Up, and Support A. Vacuoles 1. Large, saclike, membrane-enclosed structures that store materials such as water, salts, proteins, and carbohydrates. 2. Plant cells single, large central vacuole filled with liquid B. Vesicles 1. Smaller membrane-enclosed structures used to store and move materials between cell organelles of eukaryotes C. Lysosomes Lysosome Animation (lysis = to split soma=body) 1. Filled with enzymes that function as the cell’s “cleanup crew” breakdown of lipids, carbohydrates, and proteins into small molecules that can be used by the rest of the cell. 2. Sometimes called “suicide sacks” autolysis D. Cytoskeleton 1. Give Eukaryotic cells support and internal organization by a network of protein filaments 2. Microfilaments- threadlike structures that form networks in cells and produce a tough, flexible framework that also help cells move. 3. Microtubules- hollow structures made up of proteins that play critical roles in maintaining cell shape and are important in cell division 4. Centrioles- In animal cells, located near the nucleus and help to organize cell division. Organelles That Build Proteins A. Ribosomes 1. Small particles of RNA and protein found throughout the cytoplasm in all cells 2. Produce proteins by following coded instructions that come from DNA Ribosome = machine in a factory proteins DNA = boss giving orders to make proteins B. Endoplasmic Reticulum 1. In eukaryotic cells internal membrane system where lipids are made for the cell membrane, along with proteins and other materials that are exported from the cell. 2. Rough ER- involved in the synthesis of proteins It is given this name because of the ribosomes found on its surface. 3. Smooth ER- ribosomes are NOT found on its surface (therefore does not make proteins- MAKE LIPIDS!) C. Golgi Apparatus 1. Rough ER makes proteins are bundled into tiny vesicles that bud from the ER move into Golgi apparatus 2. Golgi apparatus modifies, sorts, and packages proteins and other materials from the ER for storage in the cell or release outside the cell 3. From the Golgi apparatus, proteins are “shipped” to their final destination inside or outside the cell. KNOW: The Life of a Protein Molecule The NUCLEUS holds the instructions (DNA) to make proteins translated instructions and sent out nuclear pores to cytoplasm ROUGH ER where RIBOSOMES make PROTEIN protein “buds” off into a transport VESICLE goes through cytoplasm to GOLGI APPARATUS Golgi modifies and packages protein protein buds off into a transport VESICLE goes to PLASMA MEMBRANE and fuses protein leaves the cell (exocytosis) THIS IS AN ESSAY ON THE EXAM. MEMORIZE AND BE ABLE TO EXPLAIN EACH STEP! http://wps.pearsoncustom.com/wps/media/objects/3014/3087289/Web_Tutorials/0 4_A02.swf Organelles That Capture and Release Energy A. Chloroplasts 1. Plants and some other organisms contain chloroplasts. 2. Biological equivalents of solar power plants. They capture the energy from sunlight and convert it into food that contains chemical energy in a process called photosynthesis. 3. Two membranes surround chloroplasts. 4. Inside the organelle are large stacks of other membranes, which contain the green pigment chlorophyll. B. Mitochondria 1. All eukaryotic cells, including plants, contain mitochondria. 2. Two membranes enclose mitochondria. 3. Mitochondria are the power plants of the cell. 4. Convert the chemical energy stored in food into usable compounds. ENDOSYMBIOTIC THEORY! Endosymbiotic theory proposes that MITOCHONDRIA AND CHLOROPLASTS were once prokaryotic cells. They were ingulfed by larger eukaryotic cells and began living inside the host cells. The prokaryotes may initially have been parasites or even an intended meal for the larger cell, somehow escaping digestion. Endosymbiosis Key evidence for endosymbiotic theory Mitochondria and chloroplasts divide by binary fission, just as bacteria do, and not by mitosis as eukaryotes do. Mitochondria and chloroplasts have their own single, circular DNA and their own ribosomes Mitochondria and chloroplasts are similar in size and shape to bacterial prokaryotic cells. Mitochondria and chloroplasts have similarities in membrane structure and function to prokaryotic cells. Mitochondria and chloroplasts have similar enzymes to prokaryotic cells. CELLULAR BOUNDARIES 1. Cell Wall - maintains shape of cell (only very specific things go in and out of the cell wall)2. Cell/Plasma membrane - phospholipid bilayer, regulates what goes in and out of a cell. (more goes in and out of cell membrane) Plant vs. Animal Cells DIFFERENCES 1. 2. 3. 4. 5. 6. Plant Cuboidal LARGE vacuoles Chloroplasts Cell wall NO Centrioles NO lysosomes 1. 2. 3. 4. 5. 6. Animal Spherical Few (if any) vacuoles NO Chloroplasts NO cell wall Centrioles lysosomes Plant vs. Animal Cells SIMILARITIES BOTH 1. Eukaryotic- has membrane bound nucleus and organelles (all organisms except bacteria are eukaryotic) *Bacteria are PROKARYOTIC 2. Nucleus 3. Cell (plasma) membrane 4. Golgi Body/Apparatus 5. Mitochondria 6. ER 7. ribosomes Plant Cell Anatomy Animal Cell Anatomy