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Functional Characterization of Soybean Transcription Factor GmbZIPE2 Gonçalves, A.B.; Alves, M.S.; Dadalto, S.P.; Fietto L.G. Dep. de Bioquímica e Biologia Molecular, UFV, Viçosa, MG, Brazil. INTRODUCTION: Transcription factors are proteins that regulate gene expression by binding to specific sequences in DNA. Transcription factors are among the major targets to increase the tolerance of plants to stresses, since these proteins control the expression of several genes simultaneously. Members of the bZIP family of transcription factors are characterized by having a leucine zipper domain which is a basic DNA binding domain. Previous research demonstrated that the GmbZIPE2 gene in soybean (Glyma05g30170.1) is responsive to pathogens . OBJECTIVES: The objective of this study was functionally characterize the transcription factor GmbZIPE2, by discovering which promoters binds to it, as well as determining their possible modulation of target genes. MATERIAL AND METHODS: In this research, GmbZIPE2 was amplified and cloned into a bacterial expression vector (pDEST17) and this was inserted into Escherichia coli strain C41 in which the recombinant protein was successfully expressed. The GmbZIPE2 protein was used in electrophoretic mobility shift assay (EMSA) using two cis-elements related to defense against pathogens, H-box and G-box. qRT-PCR experiments were performed to establish the relationship between the overexpression of GmbZIPE2 and expression of genes related to plant defense against pathogens. RESULTS: Our results demonstrated that GmbZIPE2 binds to H-box, but does not bind to the G-box. It was found that overexpression of GmbZIPE2 in soybean protoplasts does not interfere with the expression of GmPAL-1 and GmOMT (genes encoding proteins related to the biosynthesis of lignin and phenylpropanoid), but it activates the expression of pathogenesis-related gene 1 (GmPR1) gene which expression is induced in response to a variety of pathogens. CONCLUSIONS: These results suggest that GmbZIPE2 modulates the expression of genes related to defense against pathogens in plants. Word Keys: biotic stress, bZIP, Glycine max Supported by: CAPES, CNPq and FAPEMIG