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Biology 3340 Fall 2008 Name _____________________________________________ Read each question carefully and thoroughly! Follow directions for each section. (All multiple choice 2 points each ) 1.Select one of the following three choices to correctly complete each of the statements (1-9) given below : A. Transcription B. Replication C. Translation _____ 1. _____ 2. _____ 3. _____ 4. _____ 5. _____ 6. _____ 7. _____ 8. _____ 9. involves DNA polymerase activity. involves a transfer of amino acids to an elongating chain. requires DNA ligase to seal DNA fragments. is a DNA-dependent RNA synthesis process. involves releasing factors. begins with a start codon. requires a promoter sequence. produces Okazaki fragments is a DNA-dependent DNA synthesis process. Ans: 1. B; 2. C; 3. B; 4. A; 5. C; 6. C; 7. A; 8. B; 9. B. 10. The splitting of glucose commonly occurs by many organisms using the Embden-Myerhof pathway of glycolysis; however, it is not the only way. Bacteria such as Pseudomonas, Azotobacter and Rhizobium use another glycolytic pathway called ________ to yield 2 pyruvates, 1NADH, 1NADPH, and a net 1 ATP. ( 2 points each) A. B. C. D. E. glyoxylate pathway Citric Acid cycle Calvin-Hill-Benson cycle Entner-Duoderoff pathway Pentose phosphate shunt 11. The pentose phosphate shunt serves several purposes for the cell. Which of the following is not one of those purposes? A. generates NADPH that is used for reduction reactions in biosynthetic pathways B. generates ribose-5-P important to nucleotide and nucleic acid structures C. generates ribulose-5-P for carbon fixation reactions D. generates erythrose-4-P for aromatic amino acid synthesis and vitamin B6 synthesis E. generates glycerol for the use in phospholipid synthesis 12. What is NADH? A. an enzyme B. part of the electron transport chain C. a three carbon molecule D. an electron carrier 13. What is the Kreb's cycle "acceptor" molecule that begins the cycle? A. Oxaloacetate B. Isocitrate C. Citrate D. Pyruvate E. alpha keto glutarate 14. Glycolysis ends in the production of……… A. a single molecule of ethyl alcohol B. a single molecule of pyruvic acid C. two molecules of ethyl alcohol D. two molecules of pyruvic acid 15. What are the net end-products from glycolysis fed into the Krebs cycle and electron transport systems (ETS)? A. 2 NADH B. 2 Pyruvate C. 2ATP D. 2NADPH E. A & B. 16. Where does Krebs cycle occur? A. in the mitochondrial matrix B. in the cytoplasm C. in the chloroplast D. in the inner membrane of the mitochondria 17. Which 2-carbon molecule is produced by the preparatory steps/conversions? A. B. C. D. acetyl CoA FADH NADH Oxaloacetate 18. Electron carriers are organized in such a way that the potential energy stored in the first redox couple is released and used to form ATP. This is known as a(n)….. A. electron transport chain. B. enzymatic reduction reaction. C. enzyme transport chain. D. electron exchange reaction. Match the following definitions (19- 23) with the appropriate terms given below ( 10pts): A. Taxonomy B. Strain C. Nomenclature D. colonies E. Classification C E B A 19. The assignment of names to taxonomic groups 20. The arrangement of organisms into groups 21. A population descending from a single organism or pure culture isolate 22 The science dealing with classification D 23. small masses of bacterial cells 24. Show a schematic representation of how you will break down one molecule of glucose to water and carbon dioxide releasing energy (10 points).