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Download Hotstart Taq DNA Polymerase
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Hotstart Taq DNA Polymerase 產品編號:Bio-THM106-02 儲存條件:-20℃保存 產品包裝:Hotstart Taq DNA Polymerase , 500U (5U/ul) 10x Hotstart Taq Buffer , 1.4 mL Description: HotStart Taq DNA polymerase is designed for quantitative PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. HotStart Taq DNA polymerase is a recombinant Taq DNA polymerase which has been chemical mediated by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. HotStart Taq DNA Polymerase is activated by a 10-minute incubation at 95°C which can be incorporated into any existing thermal-cycler program. The activated enzyme maintains the same functionality as Taq DNApolymerase. Storage Buffer : 50mM Tris-HCl pH7.9, 50 mM KCL, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF, 50% Glycerol 10X reaction buffer: Buffer containing 25 mM MgCl2 Unit description: One unit is defined as the amount of enzyme that will incorporate 10n mole of dNTP into acid-insoluble material in 30 minutes at 74℃. The reaction conditions are: 50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl2, 200uM each of dATP, dCTP, dGTP, dTTP, 10mg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50 ml. Applications: •High yield amplification of complex templates. •Suitable for Real-Time PCR. •High PCR specificity–reduced effects of mispriming and primer-dimer formation. •Enhanced PCR sensitivity. •Convenient room temperature PCR set-up. •Generate PCR products with 3-dA overhangs PCR Program: Note: Annealing temperature should be 2-6℃ lower than the primer melting temperature. Shipping and Storage conditions: Shipping and temporary storage at -20℃ and for up to 1month at room temperature has no detrimental effects on the quality of Hot Start Taq DNA polymerase.