Download Lecture 17/18 - Aerobic and Anaerobic Metabolism

Chapter 9: Metabolism (p.189 – 209)
Text Vocabulary:
Glucose
Glycolysis
Citric Acid Cycle
Oxidative Phosphorylation
Cellular Respiration
Homeostasis
Substrate-level
phosphorylation
Phosphofructokinase
Cristae
Mitochondrial Matrix
Coenzyme A (CoA)
Acetyl CoA
Pyruvate Dehydrogenase
Carboxylic Acids
Guanine Triphosphate (GTP)
Electron Transport Chain
(ETC)
Ubiquinone
Coenzyme Q
ATP Synthase
Chemiosmosis
Proton-Motive Force
Anaerobic (Respiration)
Aerobic (Respiration)
Lactic Acid Fermentation
Alcohol Fermentation
Facultative Anaerobes
Lecture 17 “Cellular Respiration and Fermentation: Part I” PPT review:
1.) What are the 3 “stages” of cellular respiration?
2.) Is glycolysis an aerobic or anaerobic pathway? If you oxidize one molecule of glucose, what is
the approximate net yield of ATP?
3.) The reactions of glycolysis can all be categorized into one type of chemical reaction, what are
these reactions called? How many total reactions occur in glycolysis? What is the starting
substrate?
4.) Where in the cell does glycolysis take place?
5.) What occurs at both steps 1 and 3 in glycolysis? What enzyme catalyzes this reaction in step 1?
What enzyme catalyzes the reaction in step 3? What occurs at step 2?
6.) What happens to phosphofructokinase when ATP binds to the regulatory site? What type of
regulation is this an example of?
7.) Explain how the concentrations of ATP, AMP, Acetyl CoA, and Citrate may all play into the
regulation of PFK.
8.) What occurs at step 4? What occurs at step 5? What occurs at step 6?
9.) What specific type of phosphorylation occurs during glycolysis? Which steps does this reaction
occur at? Explain this type of phosphorylation.
10.) What is the end product of glycolysis? What is the NET yield of glycolysis from one molecule of
glucose?
11.) The product produced from glycolysis can then enter 2 pathways. What are these pathways?
12.) What redox reactions are occurring in lactic acid fermentation (reactantsproducts)?
13.) What redox reactions are occurring in alcohol fermentation (same as above)?
14.) Why is fermentation important for glycolysis?
Lecture 18 “Cellular Respiration: Part 2” PPT review:
1.) Where are most citric acid cycle enzymes located?
2.) Outline the components involved in converting Pyruvate to Acetyl-CoA. What redox reactions
are occurring? What enzyme facilitates this reaction?
3.) “Key Points” slide: Carbons donated by acetyl group are ________(oxidized or reduced?) to CO2
4.) What is the energy yield of the citric acid cycle? What type of phosphorylation produces the
GTP?
5.) Summarize the overall net energy yield for 1 molecule of glucose that undergoes glycolysis and
the CAC—It may be helpful to include the energy reactants and products
6.) Explain how the concentration of ATP would affect reaction rates—(how is CAC regulated?)
(p.162 in text)
7.) How is the energy yield from the CAC used to produce more ATP?
8.) Where in the cell does the electron transport chain occur? Are the NADH and FADH2 being
oxidized or reduced during the ETC?
9.) What is the relationship between electron movement, energy release, and proton movement in
the ETC?
10.) What type of phosphorylation produces ATP in the ETC?
11.) Outline the steps required to get electrons from both of the following molecules to Coenzyme Q
(Ubiquinone). ALSO include which complex of the ETC each is occurring in: 1.) NADH 2.) FADH2
12.) What is happening to the amount of potential energy as electrons move from the NADH or
FADH2 to the final electron acceptor?
13.) What component of Cytochrome C is important for acting as an electron carrier?
14.) From PPT example involving the consumption of hydrocyanic acid (HCN)--Which component of
the electron transport chain is inhibited (after consumption)?
15.) Outline the steps that allow ATP Synthase to catalyze the phosphorylation event of ADP + Pi 
ATP.