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Glycolysis ï· ï· ï· ï· ï· series of 10 chemical reactions occurs in the cytoplasm glucose is converted to 2 pyruvic acid molecules one 6 carbon molecule is converted to two 3 carbon molecules anaerobic processâ¦does not use oxygenâ¦will occur in the presence or ansence of oxygen 3 major phases 1. Activation of Sugar 2. Cleavage of Sugar 3. Oxidation and ATP Synthesis final product = 2 molecules of pyruvic acid 1. Sugar Activation ï· glucose is phosphorylated to Glucose-6-Phosphate (G6P) ï· G6P changes to Fructose-6-Phoshate (F6P) ï· F6P is phosphorylated to F-1,6-Bisphosphate ï· 2 ATP used for those 3 stepsâ¦.each time a P is addedâ¦..uses and ATP 2. Sugar Cleavage ï· F-1,6-Bisphosphate splits into two 3C molecules (G3P) or (DHP) (glyceraldehyde 3 phosphate or dihydroxyacetone Phosphate) 3. Oxidation and ATP Synthesis ï· six steps ï· the two G3P are oxidizedâ¦2H+ is removed from eachâ¦.picked up by 2NAD+ ï· P is then attached to each G3P ï· later the P are cleaved off and 4 ATP are created final product is: ï· 2 pyruvic acid ï· 2 NAD that picked up 2H+ eachâ¦.they are reduced to NADH2 ï· 2 ATP molecules (2 were used in phase 1, 4 were made in phase 3) glucose was C6H12O6 pyruvate is C3H4O3 and C3H4O3 soâ¦â¦4 H missingâ¦â¦bound to NAD Products of Glycolysis ATP ï· 4 are producedâ¦step 7 and step 10 ï· 2 were usedâ¦.step 1 and step 3 ï· net production = 2 ATP NADH2 ï· 2 NADH2 are made â¦step 6 ï· if O2 presentâ¦â¦NADH2 will carry those H to ETCâ¦..in ETCâ¦.each will produce 2 ATP