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Glycolysis Animation http://highered.mcgrawhill.com/sites/0072507470/student_view0/chapter25/animation__ho w_glycolysis_works.html Bridging (Transition) Process • Aerobic requires O2 as final electron acceptor (happens in ETC) • Location --inside mitochondria “One-Two Punch” • Carbonyl group released as CO2 • NAD+ reduced to NADH • Leaves Acetyl--picked up by CoA & becomes Acetyl CoA • Acetyl CoA intermediate in all catabolism (esp. fats & proteins) • Surplus of ATP acetyl-CoA gets stored as lipid • Little ATP acetyl-CoA enters Krebs cycle & makes ATP Krebs Cycle aka Citric Acid Cycle • Location--Matrix of mitochondria • Aerobic requires O2 as final eacceptor • Produces: • • • • CO2 (from carbonyl) NADH FADH2 ATP Learning Targets 5. Compare and contrast substrate level phosphorylation, oxidative phosphorylation, and photophosphorylation. Background Info: Phosphorylation • Transfer of phosphate group AND energy • Enzyme-mediated • ATP Synthase • ATPase Oxidative Phosphorylation • e- transferred to oxygen through proton pumps builds up proton gradient • Generates ATP through ATP Synthase • Occurs in inner membrane of Mitochondria Photophosphorylation • powered by Photosystems (found only in chloroplasts photosynthesis) • Final e- acceptor is NADP+ Substrate Level Phosphorylation • Phosphate transferred from a molecule (substrate) to ADP • Forms ATP direct WRITE DOWN ONLY WHAT YOU ARE UNCOMFORTABLE WITH/FEEL UNSURE ABOUT!! Do less work, not more!!! Electron Transport Chains (General) • Stepwise transfer of electrons through a series of redox reactions • Dehydrogenase removes 2 H atoms from molecule transfers 2 e- & 1 proton to NAD+ • Allows 1 H+ to diffuse into cell (building a concentration gradient WRITE DOWN ONLY WHAT YOU ARE UNCOMFORTABLE WITH/FEEL UNSURE ABOUT!! Do less work, not more!!! CR: First Electron Acceptor • NAD+ (Nicotinamide adenine dinucleotide) • NAD+ NADH CR: Final electron acceptor O2