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Answers to Review Questions - Chapter 7 1. What is the specific role of oxygen in most cells? Oxygen becomes reduced in aerobic respiration in the electron transport chain; it is the final electron acceptor. Each of the 2 atoms of diatomic oxygen accepts 2 electrons, joining with 2 hydrogen ions in the surrounding medium to produce water. 2. How much ATP is made available to the cell from a single glucose molecule by the operation of a) glycolysis, b) the formation of acetyl CoA, c) the citric acid cycle, and d) the electron transport chain and chemiosmosis? Please note whether the ATPs (if any) produced are made via substrate-level phosphorylation or oxidative phosphorylation. a) b) c) d) glycolysis – 2 ATP – substrate-level phosphorylation formation of acetyl-CoA – no ATP citric acid cycle – 2 ATP – substrate-level phosphorylation ETC/chemiosmosis – 32-34 ATP – oxidative phosphorylation 3. Why is each of the following essential to chemiosmotic ATP synthesis? a) electron transport chain - these protein complexes pump protons into the intermembrane space while passing electrons between them b) proton gradient - so that hydrogen ions will diffuse through the ATP synthase channels down their concentration gradient c) c) ATP synthase complex – as hydrogen ions pass through the synthases, the production of ATP from ADP and Pi is catalyzed, and oxygen is reduced, forming water 4. What are the roles of NAD+ and FAD in aerobic respiration? NAD+ and FAD receive electrons at varying steps during glycolysis (NAD+ only) and the citric acid cycle (both NAD+ and FAD), to form NADH and FADH2, which then carry these electrons to the electron transport chain. Oxygen acts at the end of this chain, receives the electrons, joining with hydrogen ions to produce water. 5. What is the final electron acceptor for ATP production when oxygen is present in muscle cells? How does this compare to the final electron acceptor when oxygen is insufficient (i.e. anaerobic metabolism). When oxygen is present, it will act as the final electron acceptor during ATP production in muscle cells. If oxygen is insufficient in muscle cells, an organic molecule (pyruvate) acts as the final electron acceptor, causing the formation of lactic acid.