* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download 10C Cellular respiration worksheet
Bisulfite sequencing wikipedia , lookup
Gene expression wikipedia , lookup
Genomic library wikipedia , lookup
Endogenous retrovirus wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Non-coding DNA wikipedia , lookup
Molecular cloning wikipedia , lookup
DNA supercoil wikipedia , lookup
Transformation (genetics) wikipedia , lookup
Point mutation wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Biosynthesis wikipedia , lookup
Biochemistry wikipedia , lookup
BIOLOGY CYTOLOGY Topic 1: Cellular respiration Topic 2: Glycolysis 1. What is the cellular respiration? 1. Where does the Glycolysis phase take place? 2. What is the aim of cellular respiration? 2. Glycolysis produces a net gain of ___ ATP molecules for each reaction. 3. There are two types of cellular respiration. These are 3. Glycolysis provides a cell with a net gain of ______. A) 2 ATP molecules. B) 18 ATP molecules. C) 4 ATP molecules. D) 36 ATP molecules. E) 1 ATP molecules 4. Which of the following organisms doesn’t use aerobic respiration to produce ATP energy? A) Elephant B) Mushroom C) Grass D) Bacteria E) Virus 5. Complete the general formula of Cellular Respiration. _______+ 6O2 ______+ ______+ Energy 4. What is the name of the process in which glucose is converted to pyruvate? A) Chemiosmotic Theory B) Fermentation C) Glycolysis D) Krebs Cycle E) Electron Transport System 5. Write the end products of glycolysis. In mitochondria 6. Which of organelle is used for cellular respiration in the cells? 6. On the lines provided below, write the names of the substances in the glycolysis reaction that correspond to in the diagram. 8. Write the stages of Aerobic respiration. And where does each stage take place in the cell? Steps of Aerobic Respiration Location in Cell 1. 2. 3. 4. 9. Label the parts of mitochondrion given aside. Topic 3: Pyruvate oxidation and Krebs’s cycle 1. When pyruvate is converted to acetyl CoA ___________. A) CO2 and ATP are formed B) CO2 and NADH are formed C) CO2 and coenzyme A are formed D) one turn of the Krebs cycle is completed E) (oxidized) NAD is regenerated 2. The starting molecule for the Krebs cycle is ______. Date: _____/___________/ 2012 Signature: _____________________ BIOLOGY CYTOLOGY 3. Where do the Pyruvate oxidation and Kreps cycle processes occur? 4. Write the end products of Krebs cycle. 10. Which part of the cellular catabolism of glucose requires molecular oxygen (O2)? A) Glycolysis B) Krebs cycle C) ETS D) Fermentation E) Pyruvate oxidation 6. In aerobic respiration, the Krebs cycle (citric acid cycle) takes place in _______________. 11. What is the total energy production at the end of cellular respiration from 1 molecule glucose? 7. Which of the following is not produced during the Kreps cycle? A) CO2 B) H2O C) NADH2 D) ATP E) FADH2 12. Fill in the squares with the correct ATP number. 8. The figure given below represents Pyruvate oxidation and Krebs’s cycle. Label the missing parts of figure. NADH2 Glycolysis Pyruvate Oxidation Kreb’s cycle Topic 4: ETS and Chemiosmosis 1. What is the meaning ETS? 2. At the end of the ETS the electrons are captured by _____. 3. ETS are located in ____________. 4. How many ATP are produced in ETS? 5. Each NADH2 generates ____ ATP and each FADH2 generates ____ ATP in ETS. 6. What is the main aim of ETS? 8. The energy of the electrons passing along the electron transport chain is used to make _______. A) lactic acid B) citric acid C) alcohol D) ATP E) glucose Date: _____/___________/ 2012 Signature: _____________________ FADH2 ATP NET GAIN BIOLOGY CYTOLOGY Topic 5: Catabolism of lipids and proteins Topic 6: Anaerobic Respiration 1. Catabolism of one molecule lipid produces ___________ ATP Catabolism of one molecule amino acid produces ____________ ATP Catabolism of one molecule glucose produces ____________ ATP 1. Complete the missing parts of lactic acid fermentation reaction given below. 2. When are the lipids or proteins used as energy source in body? 3. A gram of fat oxidized by respiration produces approximately _____ as much ATP as a gram of carbohydrate. A) half B) twice C) 4 times D) 10 times E) 100 times 4. In glycolysis in the absence of oxygen, cells need a way to regenerate which compound? A) ethanol B) carbon dioxide C) NAD+ D) lactate E) glucose 5. Sports physiologists at an Olympic training center wanted to monitor athletes to determine at what point their muscles were functioning anaerobically. They could do this by checking for a buildup of _____. A) ATP C) carbon dioxide E) oxygen 3. After Glycolysis three different pathways are possible for cellular respiration. They are: 4. Write the end products of Alcoholic and Lactic acid fermentation. Alcoholic Fermentation: B) lactate D) ADP 6. When protein molecules are used as fuel for cellular respiration, _____ are produced as waste. A) amino groups B) fatty acids C) sugar molecules D) molecules of lactate E) ethanol and CO2 7. What is the correct order of using organic compounds as carbohydrates, proteins, lipids in human body? __________________________________ 8. A lipid molecule is composed of a _____________ molecule bonded to three ______________ molecules. 9. What is the risk of using proteins as energy source? 10. Which kind of disease can be seen in a person who takes low amount of proteins in diet? Date: _____/___________/ 2012 2. What is the net profit of ATP, when 2 molecules glucose are used in fermentation? A) 2 B) 4 C) 6 D) 38 E) 72 Lactic Acid Fermentation: 5. Alcoholic fermentation is used in the production of _____________________________________. Lactic acid is produced in the muscles of mammals when ___________________________________. 6. Which process is used to produce beer and wine? A) Lactic acid fermentation B) glycolysis C) Alcoholic fermentation D) the Krebs cycle E) ETS 7. Muscle tissues make lactate from pyruvate in order to _____. A) enable you to get drunk B) get rid of toxic pyruvate C) utilize the energy in pyruvate D) utilize the released CO2 E) regenerate (oxidized) NAD+ 8. Why do we feel tired after sport activities? Signature: _____________________ BIOLOGY CYTOLOGY Topic 7: The Secret of Life: Nucleic acids Topic 8: DNA 1. What are the 2 main functions of nucleic acids? 1. What are the functions of DNA in cell? 2. Write the name of components of a nucleotide given below. 2. A DNA molecule includes 2000 nucleotides.There are 300 guanine in this DNA. How many hydrogen bonds are there in this DNA? Answer: 2300 3. One strand of DNA that molecule has a nucleotide sequence TAACGTA. Its complementary DNA strand will have a sequence that reads: A) AUUGCAU B) CGGTACG C) ATTGCAT D) ATGCAAT E) ATTCGAT 3. Write the units of Nucleic Acids. 4. Two nucleotides that on each strand bind with __________ bond and two nucleotides that on the same strand bind with the ______________ bond. 5. The two strands of a DNA double helix are antiparallel. This means that _____. A) the two strands are mirror images B) only one of the two strands can be used as a template for replication, since DNA polymerase only works in one direction C) one strand is actually composed of RNA D) one strand runs in the 5’ --> 3’ direction and the other runs in the 3’ --> 5’ direction E) they both run in the 3’ --> 5’ direction 6. One strand of a DNA molecule has the base sequence ATAGGT. The complementary base sequence on the other strand of DNA will be _______________. 7. Which one of the following accurately reflects complementary base pairing in the DNA molecule? A) guanine–cytosine B) guanine–adenine C) cytosine–hemanine D) uracil–thymine E) adenine–cytosine 8. Which of the following is NOT true about doublestranded DNA? A) It is helical. B) It contains phosphodiester linkages. C) The two strands are said to be complementary. D) Adenine and uracil are present in equal amounts. E) The strands run antiparallel. 4. Which of the following lists the four bases contained in DNA? A) cytosine, guanine, thymine, uracil B) adenine, guanine, hemanine, thymine C) guanine, pyroline, thymine, uracil D) adenine, guanine, purine, thymine E) adenine, guanine, cytosine, thymine 9. What is the function of Phosphodiester bonds in DNA structure? 5. Which of the following bases is the purine base? A) Thymine B) Cytosine C) Uracil D) Pyroline E) Adenine 10. Which bonds hold together double strands of DNA? 6. What is the main difference between DNA and RNA? Date: _____/___________/ 2012 Signature: _____________________ BIOLOGY CYTOLOGY Topic 9: DNA replication Topic 10: RNA 1. Mach the correct sentences with the terms given below. a) DNA ligase b) DNA polymerase c) DNA Helicase d) DNA primase e) RNA primer 1. Mach the sentences. a) mRNA: b) tRNA : c) rRNA : 1.___unwinds the two DNA strands 2.___Produce RNA primers 3.___builds the new strands in a 5’ to 3’ direction 4.___Links the Okazaki fragments 5.___starts the building of new DNA strands 2) ____ form ribosome’s with proteins 2. 3’ AATGCGTAT 5’ is the one strand of a DNA molecule. What is the sequence of the leading and lagging strand that produced in DNA replication? 1) ____ transport amino acids from cytoplasm to ribosome 3) _____ carry information from DNA to ribosome 2. AAA TTT CCC GGG is the DNA strand. Which of the following is the mRNA strand produced from DNA given above? A) TTT AAA GGG CCC B) UUU AAA GGG CCC C) UUU TTT GGG CCC D) TTT AAA CCC GGG E) AAA TTT CCC GGG 3. The triplets of nucleotides base on the mRNA are called __________. 3. What is the aim of DNA replication? The triplets of nucleotide base on the tRNA are called ___________. 4. Why are the Okazaki fragments formed during the replication? 4. Where can we find the RNA in a cell? 5. Compare DNA and RNA molecules. 5. The DNA strand being produced continuously in the 5’ --> 3’ direction is called the _____ strand. A) Okazaki B) leading C) bubble D) lagging E) new 6. Two new strands of DNA molecules grow as bases are added by the enzyme _____. A) bacterial duplicating complex B) DNA polymerase C) DNA replicase D) Okazaki enzyme E) polynucleotidase 6. Write the sites of t-RNA in figure given below. 7. What enzyme joins Okazaki fragments? A) DNA polymerase B) DNA ligase C) topoisomerase D) Helicase E) primase 8. Which enzyme unwinds the helical structure of DNA? A) DNA polymerase B) DNA ligase C) topoisomerase D) DNA Helicase E) Primase 9. What are the meanings of 5’ and 3’ terms? 10. Why does replication occur every time from 5’ to 3’? Date: _____/___________/ 2012 7. Which of the following brings the amino acids from different sides of cytoplasm to ribosome? A) DNA B) tRNA C) mRNA D) mtDNA E) rRNA Signature: _____________________ BIOLOGY CYTOLOGY Topic 11: Chromatin and Chromosome Topic 12: Cell Karyotype 1. What is the chromatin? 1. How many chromosomes are found in a human cell? 2. What components are found in the nucleosome? 2. What is the meaning of karyotype? 3. What is the difference between chromatin and chromosome? 3. Explain the following terms. Haploid chromosomes: 4. Explain the following terms: Histone: Sister chromosomes: Nucleosome: Homologous chromosomes: Chromatin: Diploid chromosomes: Chromatid: Chromosome: 5. What is the correct sequence from smaller to bigger? a- Chromatin b- DNA c- Nucleotide d- Chromosome e- Gene f- Chromatid g- Nucleosome The correct order: _______________________ 4. If an organism has 34 chromosomes, its gamete cells are _________________. A) diploid and have 17 chromosomes. B) diploid and 34 chromosomes C) haploid and have 17 chromosomes D) haploid and 34 chromosomes E) triploid and 51 chromosomes 5. Which of the following is false for human karyotype? A) Have 23 pair of chromosome B) Have 23 homologous chromosome pairs C) Have 44 autosome and 2 sex chromosome D) Contain diploid cells E) 46 chromosome are found in the gamete cells 6. An egg cell of human karyotype is symbolized as 6. If the centromere is located at the mid-point and each chromosome arm is of equal length, this chromosome is called ___________. A) Metasentric B) submetacentric C) telocentric D) Acrocentric E) centrocentric 7. A nucleosome composes of __________. A) A gene and centromere B) DNA and histone protein C) DNA and RNA D) centromere and histone protein E) Only DNA 8. The region of a chromosome holding the two double strands of replicated DNA together is called _____. A) chromatin B) a centriole C) a centromere D) a chromatid E) an aster 9. The complex of DNA and protein that makes up a chromosome is properly called _____. A) a chromatid B) a chloroplast C) chromatin D) a chromoplast E) a chromagen Date: _____/___________/ 2012 _______ and a sperm cell symbolized as ___________ or ____________. 7. Why a cell divides into two? Write 2 reasons. 8. A human somatic cell contains _____ chromosomes. A) 23 B) 47 C) 22 D) 46 E) 44 9. If an intestinal cell in a grasshopper contains 24 chromosomes, a grasshopper sperm cell would contain ____ chromosomes. A) 3 B) 6 C) 12 D) 24 E) 48 10. What are the effects of X and Y chromosomes to human? Signature: _____________________ BIOLOGY CYTOLOGY Topic 13: Mitosis 11. Write the correct names of phases of mitosis. 1._____________ is a period from the beginning of one division to the beginning of the next division 2. DNA replication occurs in ___________. A) G1 phase B) S phase C) G2 phase D) prophase E) telophase 3. Chromatids are formed _____. A) during G1 B) during G2 C) during the S phase D) at the start of mitosis E) at the cytokinesis 4. The main functions of mitosis are _________ , __________ and _________________ 5. Explain the following phases of Cell cycle. G1: S: G2: Mitosis: 6. How many chromosomes are present in a somatic human cell in G1? A) 23 B) 46 C) 92 D) 184 E) none of the above 7. Sister chromatids separate during ___________. A) anaphase B) G1 phase B) G2 phase D) metaphase E) prophase 12. Sister chromosomes are separated from each other through the each pole in __________ phase. 8. In animal cell mitosis, the cleavage furrow forms during ___________. A) anaphase B) G1 phase C) cytokinesis D) metaphase E) prophase 13. If you want to observe the chromosomes better, in which phase must be observed? Why? 9. Complete the phases of cell cycle in the given picture. 14. Single sister chromatids are found in cells at mitotic _____. A) prophase and telophase B) prophase and anaphase C) prophase and metaphase D) metaphase and anaphase. E) anaphase and telophase 15. Which of the following is false about mitosis cell division? A) Produce 2 cells. B) Daughter and parent cell are identical C) Chromosome number remains constant D) Seen in body cells E) It decreases chromosome number to haploid (n). 10. If a human muscle cells divide mitotically 5 times, what will be expected the number of daughter cells and their chromosome number? A) 32-23 B) 10-23 C) 32-46 D) 32-10 Date: _____/___________/ 2012 16. "Cytokinesis" refers to _____. A) division of the entire cell B) division of the nucleus C) division of the cell outside the nuclear material D) reduction in the number of chromosomes E) cell movement Signature: _____________________ BIOLOGY CYTOLOGY Topic 14: Meiosis 1. What is the aim of the Meiosis? 2. How many egg cells are produced at the end of meiosis? 3. Which of the following is not correct for meiosis? A) It reduces the chromosome numbers into half B) Four cells form by one meiotic division C) Occurs only in body cell D) Occurs in reproductive cells E) Involves two divisions 9. Which of the following best describes the meiosis? A) It is carried out in all tissues that require cell replacement. B) It occurs only in cells in the reproductive structures of the organism. C) It happens in all tissues except the brain and spinal cord. D) It is the first stage of mitosis. 10. Which of the following is true for meiosis II? A) Sister chromatids separate each other B) Crossing over is observed in prophase II C) Chromosome number increase during the metaphase II D) Meiosis II occurs in somatic cells E) After the meiosis II daughter cells can divide many times again. 4. Why do cells divide in meiosis two times? 11. If an organism receives 12 chromosomes from its father, how many chromosomes can be expected in its brain cells? A) 12 B) 24 C) 64 D) 36 E) 38 5. Which of the following occurs during anaphase I of meiosis? A) Formation of tetrads B) Separation of homologous chromosomes C) Crossing over D) Separation of sister chromatids, with one chromatid pulled toward each pole E) Line up of chromosomes along the equatorial plane 6. What is the crossing-over? In which phase does it occur? 12. Rice gamete cells contain 12 chromosomes. How many chromosomes do rice diploid cells have? A) 3 B) 6 C) 12 D) 24 E) 48 13. What is the typical result when a diploid cell undergoes meiosis? A) two diploid cells B) two haploid cells C) four diploid cells D) four haploid cells E) two haploid cells and two diploid cells 14. Mitosis and cytokinesis result in the formation of ______; meiosis and cytokinesis result in the formation of ________. A) four diploid cells ... four haploid cells B) two diploid cells ... two haploid cells C) two diploid cells ... four haploid cells D) two diploid cells ... two diploid cells E) four haploid cells ... two diploid cells 7. Fill in the blanks in figure given below. 15. What are the differences between Mitosis and Meiosis divisions? 16. Why does chromosome number of cells decrease half of parent cell’s chromosomes? 17. Where do Meiosis divisions occur in the body? 8. At which stage of Meiosis are chromosomes lined up in one plane in preparation for their separation to opposite poles of the cell? A) prophase I B) metaphase I C) anaphase I D) telophase I E) interphase Date: _____/___________/ 2012 18. What is the effect of crossing-over at the variation of organism? Signature: _____________________ BIOLOGY CYTOLOGY Topic 15: Protein Synthesis 11. Which of the following codons can not code a functional amino acid? A) UAC B) UAA C) GUG D) AUG E) UGG 1. Why do we need to synthesize proteins? 2. What is the function of DNA in protein synthesis? 3. Which of following carries information coded in DNA to ribosomes? A) tRNA B) mRNA C) rDNA D) rRNA E) mtDNA 4. Explain the terms of transcription and translation. 5. When RNA is being made, the RNA base ____ always pairs with the base _____ in DNA. A) U ... T B) T ... G C) U ... A D) A ... U E) T ... A 6. If the each amino acid is coded by 3 bases (codon) of 4 bases (ATGC), there must be 64(43) amino acid. But we have only 20 kind of amino acid. Write the reason. 12. I- m-RNA is attached to ribosomes II- m-RNA moves from the nucleus to cytoplasm III- t-RNA molecules carring aminoacids arrange themselves along the mRNA template IV- m-RNA separates from the DNA strand along which it was synthesized V- m-RNA separates from ribosome Which of the following represent the correct sequence of the steps listed above, leading to the formation of a protein in a eukaryotic cell? A) IV-III-I-II-V B) I-IV-V-II-III C) I-IV-III-II-V D) IV-I-II-V-III E) IV-II-I-III-V 13. How many amino acids can be synthesized by mRNA formed from one strand of DNA which has 4200 total Hydrogen bond and 300 Adenine in protein synthesis? 14. The DNA code is CGT. What messenger RNA is made from this? A) ATU B) GCA C) TCU D) CTA E) UCG 15. The translation process in eukaryotes requires all of the following, EXCEPT _____. A) ribosomes B) RNA polymerase C) aminoacyl tRNA synthetase enzymes D) transfer RNA E) AUG codons 16. Codons Found in Messenger RNA given in table. 7. 5' TACAGCGCTGGC 3' The above sequence of DNA is part of a gene. How many amino acids are coded for by this segment? A) 4 B) 8 C) 12 D) 20 E) 23 8. How many nucleotides are needed to code for a protein with 450 amino acids? A) at least 150 B) at least 300 C) at least 450 D) at least 900 E) at least 1,350 9. Which of the following is NOT associated with RNA? A) ribose B) thymine C) uracil D) phosphates E) single-strandedness 10. Which of the following is false about protein synthesis? A) before synthesis DNA must be unzipped B) code of RNA are given by antisense strand of DNA C) transcription started by RNA polymerase D) mRNA strand is identical to antisense DNA strand except, instead of thymine to uracil E) tRNA can transfer only one type of amino acid Date: _____/___________/ 2012 A strand of mRNA containing the repeating sequence 5’AAGAAGAAGAAG3’ could code for which of the following amino acid sequences? A) lys–arg–glu–lys B) ser–ser–glu–glu C) lys–arg–lys–arg D) lys–lys–lys–lys Signature: _____________________