* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download CoA
Genetic code wikipedia , lookup
Lactate dehydrogenase wikipedia , lookup
NADH:ubiquinone oxidoreductase (H+-translocating) wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Peptide synthesis wikipedia , lookup
Point mutation wikipedia , lookup
Lipid signaling wikipedia , lookup
Photosynthesis wikipedia , lookup
Evolution of metal ions in biological systems wikipedia , lookup
Basal metabolic rate wikipedia , lookup
Metalloprotein wikipedia , lookup
Oxidative phosphorylation wikipedia , lookup
Adenosine triphosphate wikipedia , lookup
15-Hydroxyeicosatetraenoic acid wikipedia , lookup
Nicotinamide adenine dinucleotide wikipedia , lookup
Amino acid synthesis wikipedia , lookup
Specialized pro-resolving mediators wikipedia , lookup
Butyric acid wikipedia , lookup
Biochemistry wikipedia , lookup
Biosynthesis wikipedia , lookup
Glyceroneogenesis wikipedia , lookup
Biosynthesis of doxorubicin wikipedia , lookup
Citric acid cycle wikipedia , lookup
BIOC 460 DR. TISCHLER LECTURE 34 SYNTHESIS & PROCESSING OF FATS OBJECTIVES 1. Sequence leading from glucose to fatty acids via lipogenesis including roles of pyruvate carboxylase and pyruvate dehydrogenase. 2. Malic enzyme and acetyl CoA carboxylase 3. For fatty acid synthase: a) substrates/key products; b) sources of NADPH; c) general mechanism 4. Relationship: regulation of carnitine-palmitoyl transferase-I and preventing oxidation of synthesized palmitoyl CoA 5. Eicosanoids: a) fatty acid from which they are derived; b) specific functions of each eicosanoid; c) general pathway of production; effects of glucocorticoids (cortisol) and aspirin LIPOGENESIS principally in adipose tissue and liver lipogenesis – cytoplasm; requires acetyl CoA adipose: FA stored as triacylglycerols via esterification liver: produces TAG packaged into VLDL and exported compounds metabolized to acetyl CoA can serve as a fat precursor glucose = primary source of carbons for fat synthesis. Glucose CYTOPLASM Fatty Acids PPP MITOCHONDRIAL MATRIX NADH, CO2 NAD, CoA Glycolysis PDH CO2 NADPH FAS Malate ME NADP+ NAD+ Malonyl CoA MDH ADP, Pi ACC NADH CO2, ATP Oxaloacetate Acetyl CoA ADP+Pi CL Pyruvate ATP, CO2 Pyruvate Acetyl CoA PC ADP, Pi Oxaloacetate CS ATP, CoA Citrate Citrate Figure 1. Export of acetyl CoA as citrate for fatty acid biosynthesis, generation of NADPH and pathway of lipogenesis. KEY MITOCHONDRIAL REACTIONS PYRUVATE CARBOXYLASE pyruvate + CO2 + ATP oxaloacetate + ADP + Pi PYRUVATE DEHYDROGENASE pyruvate + NAD + coenzyme A (CoA) acetyl CoA + CO2 + NADH KEY CYTOPLASMIC REACTIONS INDIRECTLY NEEDED FOR LIPOGENESIS Citrate Lyase citrate + CoA + ATP acetyl CoA + oxaloacetate + ADP + Pi Malate dehydrogenase oxaloacetate + NADH malate + NAD+ Malic Enzyme malate + NADP+ pyruvate + NADPH KEY CYTOPLASMIC REACTIONS DIRECTLY NEEDED FOR LIPOGENESIS AND FATTY ACID ACTIVATION Acetyl CoA Carboxylase: acetyl CoA + HCO3- + ATP malonyl CoA + ADP + Pi Fatty Acid Synthase: acetyl CoA + 7 malonyl CoA + 14 NADPH + 14 H+ palmitate + 7 CO2 + 8 CoA + 14 NADP+ Acyl CoA Synthetase: (also used for fatty acids other than palmitate) palmitate + ATP + CoA palmitoyl CoA + AMP + PPi CE acp A C P condensation CE acp CO2 CO2 C=O C=O CO2 CH2 CHC=O 3 C=O C=O CH3C=O - CO2 COO CH CH2 2 CH 3 acetyl malonyl C=O C=O CoA CoA CH3 CH3 A C P reduction dehydration reduction 2 NADPH C=O C=O CH2 CH2 C=O C=O CH3 CH3 CE acp A C P 2 NADP+ C=O CH2 CH2 CH3 4-C unit Figure 2. General mechanism for the fatty acid synthase reaction. CE is condensing enzyme. ACP is acyl carrier protein. This row represents the initial steps for priming the reaction with acetyl CoA and the addition of two carbons from malonyl CoA. CE acp A C P condensation CE acp A C P 2 NADPH CO2 4-C unit malonyl CoA reduction dehydration reduction 6-C unit 2 NADP+ CE acp A C P 6-C unit Figure 2. General mechanism for the fatty acid synthase reaction. CE is condensing enzyme. ACP is acyl carrier protein. This row depicts a typical cycle of adding two more carbons to the fatty acid chain. CE acp 6-C unit A C P malonyl CoA 5 more cycles adding 10 more carbons 5malonyl CoA 5CO2 10NADPH 10NADP+ CE acp A C P thioesterase cleavage 16-C unit palmitate palmitate CE acp Figure 2. General mechanism for the fatty acid synthase reaction. CE is condensing enzyme. ACP is acyl carrier protein. This row shows the release of the finished product, palmitate, through cleavage by thioesterase. A C P Sources of NADPH for the Biosynthesis of Fatty Acids. malic enzyme: Malate + NADP+ Pyruvate + CO2 + NADPH pentose phosphate pathway: Glucose-6-P + 2 NADP+ Ribulose-5-P + 2 NADPH + CO2 Glycerol ATP ADP Dihydroxyacetone phosphate fatty acyl CoA glycerol kinase CoA Glycerol-3-P fatty acyl CoA Acyldihydroxyacetone phosphate NADPH CoA Lysophosphatidic acid fatty acyl CoA NADP+ Pi CoA Phosphatidic Diacylglycerol phosphatase acid fatty acyl CoA CoA Triacylglycerol Figure 3. Formation of phosphatidic acid from glycerol-3-P or DHAP, and its conversion to triacylglycerol EICOSANOIDS hormones localized to tissues where they are produced. prostaglandins, thromboxanes and leukotrienes. derived from arachidonic acid arachidonic acid from linoleic acid an essential fatty acid Table 1. Physiological functions of eicosanoids. Eicosanoid Functions prostaglandins inflammation, fever production, prevent platelet aggregation (prevent clotting); induce labor thromboxanes produced by platelets to promote their aggregation (blood clotting) leukotrienes allergic reactions Membrane Phospholipid Phospholipase A2 inhibited by glucocorticoids Leuokotrienes Arachidonic acid Lipoxygenase Cyclooxygenase inhibited by aspirin, ibuprofen PGH2 Thromboxanes in platelets Prostaglandins in many cells Figure 4. Conversion of arachidonic acid to eicosanoids.