Download Annexure `CD-01` L T P/S SW/FW TOTAL CREDIT UNITS 3 0 0 0 3

Annexure ‘CD-01’
Course Title: BASIC BIOCHEMISTRY
Course Code: CHEM 311
Credit Units: 3
Level: Under Graduate
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P/
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SW/F
W
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CREDIT
UNITS
3
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3
Course Objectives: • The course is designed to enable the students to develop a sound knowledge of
fundamental concepts in biochemistry. This will also help the students to enumerate the molecular motif of
a living cell, structural and functional hierarchy of biomolecules besides emphasis on the various aspects of
metabolism and interrelationship of metabolic events.
Prerequisites:
Basic concept of biology
Course contents/Syllabus
Weightage (%)
Module I
Amino acids and Proteins
25
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Amino acids
classification & Synthesis of -amino acids and their identification.
Peptide bond- stereochemistry, synthesis of peptides by solution and solid phase techniques.
Proteins
classification – properties-3D structure-determination of amino acid sequence –denaturation and
renaturation of protein molecules.
 Separation and purification of proteins – dialysis – gel filtration - electrophoresis.
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 Catabolism of amino acids: Transamination, oxidative deamination, decarboxylation.
Module II
Enzymes
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Nomenclature, classification and properties-specificity,
factors influencing enzyme action.
Mechanism of enzyme action – Lock and Key model and induced fit models.
Coenzymes – cofactors – prosthetic groups of enzymes (TPP, NAD, NADP, FAD, ATP).Their
importance in enzyme action.
Mechanism of inhibition (competitive, non- and uncompetitive and allosteric) Immobilization of
enzymes. Enzyme specificity,
Module III
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
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20
Carbohydrates
25
Classification – reducing and non-reducing sugars. Glucose: structure-conformation –stability
Carbohydrates of the cell membrane – starch, cellulose and glycogen. (Structure and utility)
Metabolism: Glycolysis and its reversal; TCA cycle. Relation between glycolysis and respiration.
Principles of bioenergetics, electron transport chain and oxidative phosphorylation.
Module V
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Lipids
Classification - neutral lipids, Phospho lipids (lecithines, cephalins, plasmalogens) and glycolipids –
importance, synthesis and degradation.
Fatty acids – saturated, unsaturated fatty acids, EFA.
Properties – Hydrolysis-acid number, saponification number. Auto-oxidation (Rancidity), addition
reactions-Iodine value, Polenske number, Reichert-Meissl number, acetyl number. Hydrogenation
Module IV
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
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15
Nucleic Acids
Nucleosides and nucleotides – purine and pyrimidine bases.
Nucleic acids Difference between DNA and RNA.
Classification of RNA.
Biosynthesis of DNA: Replication.
15
15
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
Biosynthesis of mRNA: Transcription.
Genetic code – mutations and mutants. DNA repair. Biosynthesis of proteins.
Student Learning Outcomes: After successfully completing this unit, the student should
1. Be able to apply knowledge of chemistry and biology to solve biochemical problems.
2. .Students will be able to distinguish among carbohydrates, lipids, proteins, and nucleic acids with respect to chemical structure
3. Be able to: demonstrate an understanding of the properties of biomolecules and be able to predict behaviour of molecules from structures.
4. Students will know the reactions of major metabolic pathways (Central Metabolism: Glycolysis-Gluconeogenesis, Pentosephosphate Patthway, Citric Acid
Cycle, Respiratory Electron Transport System, oxidative phosphorylation) and. Know the order of metabolic intermediates and the corresponding enzyme
names for the pathways.
Pedagogy for Course Delivery: This will be done in the form of lectures and tutorials. Students will be
judged in the form of tests, assignments quizzes & presentations.
Assessment/ Examination Scheme:
Theory L/T (%)
Lab/Practical/Studio (%)
100%
End Term Examination
NA
100
Theory Assessment (L&T):
Continuous Assessment/Internal Assessment
Components
(Drop down)
CT
HA
S/V/Q
Attendance
End Term
Examination
Weightage
(%)
10
8
7
5
70
Text & References:
 Lehninger, Principles of Biochemistry, Fourth Edition, by David L. Nelson and Michael M. Cox, Worth Publishers, New York, 2005.
 L. Veerakumari, Biochemistry, MJP publishers, Chennai, 2004.
 Lubert Stryer, Biochemistry, W. H. Freeman and company, New York, 1975.
 Robert L.Caret, Katherine J. Denniston, Joseph J. Topping, Principles and Applications of organic and biological chemistry, WBB publishers, USA, 1993.
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J. L. Jain, Biochemistry, Sultan Chand and Co.1999
A. Mazur and B. Harrow, Text book of biochemistry, 10th Edition, W.B. Saunders Co., Philadelphia, 1971.
Paula Yurkanis Bruice, Organic chemistry, 3rd Edition, Pearson Education, Inc. (Singapore), New Delhi, reprint, 2002.
P. W. Kuchel and G. B. Ralston, Shaum Series, Theory and Problems of Biochemistry, McGraw-Hill Book Company, New York, 1988.