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Download DNA Ladder - Swift Analytical
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MR19 A+B DNA Ladder MR19 A+B DNA Ladder The A+B molecular weight DNA ladder is designed for determining the size of large and medium PCR products and dsDNA fragments in the 500 - 6000 bp range. The A+B DNA Ladder is a mixture of specially selected, purified, blunt-ended PCR products. The A+B DNA Ladder contains two ladders, A DNA Ladder and B DNA Ladder, which can be combined in one broad range ladder with a highlighted band (1000 bp). A DNA loading buffer (6x GREEN) is provided with each DNA ladder. The loading buffer contains Ficoll® 400, which facilitates rapid sinking to the bottom of the well in the agarose or polyacrylamide gel. The solution protects DNA thanks to the presence of EDTA, which inactivates nucleases and other enzymes by binding the divalent metal ions crucial to their activity. The 6xGREEN solution contains two different dyes which migrate in the electrophoretic field differently from the standard size PCR products, ensuring that they do not hinder the analysis by image obscuration. The orange G dye corresponds to 50 bp and xylene cyanol FF to 4000 bp PCR products in a 1% agarose gel. Features and advantages pp Allows precise determination of DNA fragments in a wide size range pp DNA Ladder A and DNA Ladder B can be used as three different ladders: A DNA Ladder, or B DNA Ladder for short electrophoresis runs, and A+B DNA Ladder pp The combined A+B DNA Ladder contains one band (1000 bp) with the highest intensity, which allows easy identification pp Sharp bands are obtained after electrophoresis pp Suitable for gels stained with ethidium bromide, SYBR Green or other stain for DNA visualization DNA-Gdańsk | [email protected] | www.dnagdansk.com A+B DNA Ladder A B bp A+B #MR19 bp 6000 3000 6000 3500 3500 3000 2500 2500 2000 1000 bp xylene cyanol FF 2000 1500 1500 1000 1000 750 750 500 orange G 500 DNA fragment sizes (bp) A DNA Ladder: 6000, 3000, 2000, 1000, 500 B DNA Ladder: 3500, 2500, 1500, 1000, 750 Usage pp For the best results, apply approx. 5 µl of A DNA Ladder and B DNA Ladder or 10 µl of A+B DNA Ladder per well. pp Mix 1 volume of DNA loading dye with 5 volumes of analyzed DNA and/or DNA ladder before application. A+B DNA Ladder Components MR19 50-100 applications A DNA Ladder 250 μl B DNA Ladder 250 μl 6x GREEN DNA Loading Buffer 500 μl Additional information Storage buffer 10 mM Tris-HCl (pH 7.6), 1 mM EDTA 6x GREEN DNA Loading Buffer 20 mM Tris-HCl (pH 7.6), 60 mM EDTA, 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 Shipping conditions Shipping at room temperature Storage conditions Keep at +4°C or room temperature for short-term or at -20°C for long-term storage. Quality control The DNA ladders have been tested in various kinds of agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures. For research use only Date of purchase DNA-Gdańsk [email protected] | www.dnagdansk.com Warranty 12 months from the date of purchase