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SAN DIEGO MESA COLLEGE SCHOOL OF NATURAL SCIENCES General Microbiology (Bio 205): Instructor: Elmar Schmid, Ph.D. Q QU UIIZZ ##55 Answer the following questions and mark it on a Scantron 882-E answer sheet. All questions must be answered by the student without help by class mates, friends or other human beings. You are of course allowed (and I encourage you) to work with your textbook (and if necessary the selected chapters of my website) to find the correct answers. Make sure that you read the following statement below very carefully and sign it if you satisfy the guidelines outlined in this statement. This signed take home exercise sheet (= page 1 of this document) must be turned in together with your completed Scantron card in order to receive full credit for this assignment. The deadline for this assignment is announced in the “Microbiology” section of my website. I HAVE ANSWERED THE QUESTIONS IN THIS EXERCISE ON MY OWN. I DID NOT CONSULT OTHERS OR IN ANY CONDUCT IN ANY ACTIVITITES THAT COULD BE CONSTRUED AS CHEATING IN THIS ASSIGNMENT. I DID NOT MAKE ANY COPIES OF THIS ASSIGNMENT. _________________________________ (PRINT YOUR NAME) ________________________________ (YOUR SIGNATURE) Q. 1: There are no redox reactions happening during glycolysis and no carbon dioxide is released as waste product. A) True B) False Q. 2: Glycolysis is an oxygen-independent cellular metabolic pathway, which virtually takes place in all living cells on planet earth. A) True B) False Q. 3: The overall or so-called net equation for the cellular respiration of glucose is A) C6H12O6 + 6 CO2 6 O2 + 6 H2O + energy B) C6H12O6 + 6 O2 6 CO2 + 6 H2O + energy C) C6H12O6 + 6 O2 3 CO2 + 9 H2O + energy D) C6H12O6 + 3 CO2 3 O2 + 6 H2O + energy E) C6H12O6 + O2 3 CO2 + 6 H2O + energy Q. 4: Which of the following is/are (a) regulatory key enzyme(s) of the glycolysis pathway? A) citrate synthase B) Glyceraldehyde phosphate dehydrogenase (GAP-DH) C) Aldolase D) Phosphofructokinase (PFK) E) both, b and d help to regulate glycolysis Q. 5: There is a net production of ___ ATP molecules for every molecule of glucose broken down by glycolysis. A) zero B) 2 C) 4 D) 8 E) 38 1 SAN DIEGO MESA COLLEGE SCHOOL OF NATURAL SCIENCES General Microbiology (Bio 205): Instructor: Elmar Schmid, Ph.D. Q. 6: Which of the following metabolic pathways is common to both aerobic and anaerobic processes of sugar breakdown? A) Krebs cycle B) electron transport chain C) conversion of pyruvate to lactic acid D) conversion of glucose to pyruvate E) none of the above Q. 7: Which of the following is NOT true of glycolysis? A) FAD is reduced to FADH2 B) there is no release of CO2 C) glucose is converted into two three-carbon compounds (= GAP & DAP) D) there is a net gain of two ATPs per glucose molecule E) oxygen is not required Q. 8: The bridging chemical reaction which links glycolysis and the Krebs cycle is A) the conversion of pyruvate to acetyl-CoA B) the oxidation of NADH C) the oxidation of FADH2 D) the conversion of ADP to ATP E) the production of alcohol Q. 9: The key intermediary product associated with the pentose phosphate pathway is: A) glucose 1,6 bisphosphate B) 6-phosphogluconolactone C) dihydroxyacetone phosphate D) 2-keto-3-deoxy-6-phosphogluconate (KDPG) E) citrate Q. 10: The pentose phosphate pathway generates reduction equivalents, i.e. NADPH + H+, from glucose which are important for cellular biosynthetic (anabolic) pathways. A) True B) False Q. 11: Which of the following metabolic intermediates is/are typical for the Entner-Doudoroff pathway? A) 2-keto-3-deoxy-6-phosphogluconate (KDPG) B) Ribulose-5-phosphate C) 6-phosphogluconate D) Phosphoenolpyruvate (PEP) E) both, b and c Q. 12: The Entner-Doudoroff pathway does NOT generate ATP for a bacterial cell, but only reduction equivalents in form of NADH + H+. A) True B) False Q. 13: The key enzyme which converts pyruvate into acetyl-CoA in the strictly anaerobic Clostridia bacteria is called A) pyruvate dehydrogenase (Pyr-DH) B) pyruvate:ferredoxin oxidoreductase C) pyruvate oxidase D) pyruvate-formate lyase E) none of the above 2 SAN DIEGO MESA COLLEGE SCHOOL OF NATURAL SCIENCES General Microbiology (Bio 205): Instructor: Elmar Schmid, Ph.D. Q. 14: The formate fermentation pathway is associated with which of the following bacterial group? A) Enterobacteria B) Clostridia C) Pseudomonads D) Archaea bacteria E) all of the named ones Q. 15: Which of the following bacterial genera are known to produce gas? A) Clostridia B) Enterobacter C) Pseudomonas D) Bacillus E) both, a and b Q. 16: Which of the following is NOT a typical bacterial fermentation end product? A) ethanol B) lactic acid C) propionic acid D) citric acid E) formic acid Q. 17: The first product of the Krebs cycle is a __ -carbon molecule called _______ . A) 4 … oxalate B) 4 … malate C) 5 … succinate D) 6 … citrate E) 6 … fumarate Q. 18: In cells of eukaryotic organisms, the steps of the Krebs cycle happen in the ________ of an organelle called ______________ . A) cristae … chloroplast B) matrix … lysosome C) matrix … mitochondrion D) cytosol … mitochondrion E) lumen … rER Q. 19: The mitochondrial electron transport chain is comprised of ___ major complexes, of which complex ___ oxidizes NADH + H+ back to NAD+. A) 3 … II B) 4 … I C) 4 … II D) 4 … IV E) 5 … II Q. 20: In the mitochondrial electron transport chain, complex IV oxidizes molecular oxygen to water. A) True B) False Q. 21: Functional electron transport chains are only found in eukaryotic cells but not in bacteria. A) True B) False Q. 22: There is not net production of ATP in the Krebs cycle, all ATP is either generated during glycolysis or at the electron transport chain. A) True B) False 3