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Consortium for Educational Communication Glossary ABA: A plant growth regulator involved in the fruit ripening process. Acetyl CoA: Acetyl coenzyme A or acetyl-CoA is an important molecule in metabolism, used in many biochemical reactions. Its main function is to convey the carbon atoms within the acetyl group to the citric acid cycle to be oxidized for energy production. Actinomycin D: In cell biology, Actinomycin D is shown to have the ability to inhibit transcription. It does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase Aldehyde: is an organic compound containing a formyl group. This functional group, with the structure R-CHO, consists of a carbonyl center (a carbon double bonded to oxygen) bonded to hydrogen and an R group, which is any generic alkyl or side chain. Aleurone layer: is a protein found in protein granules of maturing seeds and tubers. The term also describes one of the two major cell types of the endosperm, the aleurone layer. The aleurone layer is the outermost layer of the endosperm, followed by the inner starchy endosperm. α-amylase: α-Amylase is an enzyme which has starch and glycogen, yielding glucose and maltose. It is the major form of amylase found in humans and other mammals. It is also present in seeds containing starch as a food reserve, and is secreted by many fungi Alleles: An allele is one of two or more forms of a gene or a genetic locus (generally a group of genes). The form “allel” is also used, an abbreviation of allelomorph. Amo-1618: is 2-Isopropyl-4-dimethylamino-5-methylphenyl-1piperidinecarboxylate methyl Chloride.an inhibitors of gibberellin biosyntheses. Ancymidol: an inhibitor of gibberellin biosyntheses. Angiosperms: Derived from the words for “vessel” and “seed”; a grouping of plants whose seeds are borne in protective structures. Consortium for Educational Communication Antheridia: is a haploid structure or organ producing and containing male gametes (called antherozoids or sperm). Auxin: A plant growth regulator that controls cell elongation; important in many plant growth responses such as phototropism and geotropism. Bekanae: from the Japanese for “foolish seedling”, is a disease that infects the rice plant. It is caused by the fungus Gibberella fujikuroi, the metabolism of which produces a surplus of gibberellic acid. In the plant, this acts as a growth hormone, causing hypertrophy. The afflicted plants, which are visibly etiolated and chlorotic, are at best infertile with empty panicles, producing no edible grains; at worst, they are incapable of supporting their own weight, topple over, and die (hence “foolish seedling disease”). Biosynthesis: Biosynthesis (also called biogenesis or “anabolism”) is an enzyme-catalyzed process in cells of living organisms by which substrates are converted to more complex products. Bolting: Bolting is when plants grown in horticulture and agriculture for their edible leaves (such as cabbage, lettuce, spinach, and other leafy greens), instead prematurely (before the crop is harvested) produce a flowering stem (or stems) and ultimately seeds, in order to reproduce Cell wall extensibility: Expansive growth of plant cells is controlled principally by processes that loosen the wall and enable it to expand irreversibly Colchicines: is a medication that treats gout. It is a toxic natural product and secondary metabolite, originally extracted from plants of the genus Colchicum (autumn crocus, Colchicum autumnale, also known as “meadow saffron”). Copalyl pyrophosphate synthase: This enzyme belongs to the family of isomerases, specifically the class of intramolecular lyases. The systematic name of this enzyme class is ent-copalyldiphosphate lyase (decyclizing). Other names in common use include ent-kaurene synthase A, and ent-kaurene synthetase A. Consortium for Educational Communication Cycloheximide: Cycloheximide: is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome) thus blocking translational elongation. Cycocel (CCC): (chlormequat chloride) reduces plant height by inhibiting the production of gibberellins, the primary plant hormones responsible Dark-grown protonemata: A protonema (plural: protonemata) is a thread-like chain of cells that forms the earliest stage (the haploid phase) of a bryophyte life cycl. Decarboxylation: Decarboxylation is a chemical reaction that removes a carboxyl group and releases carbon dioxide (CO2). Usually, decarboxylation refers to a reaction of carboxylic acids, removing a carbon atom from a carbon chain. The reverse process, which is the first chemical step in photosynthesis, is called carbonatation, the addition of CO2 to a compound. Enzymes that catalyze decarboxylations are called decarboxylases or, the more formal term, carboxy-lyase. DELLA proteins: DELLA proteins are negative regulators of gibberellin (GA) signaling that act immediately downstream of the GA receptor. Dioecious flowers Describes plants in which the male and female flowers occur on separate plants. Diterpene: Diterpene, a type of terpene, is an organic compound composed of four isoprene units and has the molecular formula C20H32. They derive from geranylgeranyl pyrophosphate. Diterpenes form the basis for biologically important compounds such as retinol, retinal, and phytol. Dynamic equilibrium: A dynamic equilibrium exists once a reversible reaction ceases to change its ratio of reactants/products, but substances move between the chemicals at an equal rate, meaning there is no net change. It is a particular example of a system in a steady state. Endosperm: A temporary food storage tissue in seeds, created Consortium for Educational Communication by the fusion of one sperm cell plus the two polar nucleu, and therefore often triploid. Ent-gibberellane skeleton Expansins: Expansin refers to a family of closely related non enzymatic proteins found in the plant cell wall, with important roles in plant cell growth, fruit softening, abscission, emergence of root hairs, pollen tube invasion of the stigma and style, meristem function, and other developmental processes where cell wall loosening occurs Expression: The way a trait shows up in an organism Gene expression: Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as ribosomal RNA (rRNA), transfer RNA (tRNA) or small nuclear RNA (snRNA) genes, the product is a functional RNA. Genotype: The genotype is the genetic makeup of a cell, an organism, or an individual (i.e. the specific allele makeup of the individual) usually with reference to a specific character under consideration.[ Geranylgeranyl pyrophosphate: Geranylgeranyl pyrophosphate is an intermediate in the HMG-CoA reductase pathway used by organisms in the biosynthesis of terpenes and terpenoids In plants it is also the precursor to carotenoids, gibberellins, tocopherols, and chlorophylls. Gibberellins: A plant growth regulator that controls the elongation of internodes Gymnosperms: Literally, “naked seed”, a grouping of plants that produces seed that is borne exposed, rather than in a protective structure. Hormones: is a chemical released by a cell or a gland in one part of the body that sends out messages that affect cells in other parts of the organism. Only a little amount of hormone is required to alter cell metabolism Hydroxylation: Hydroxylation is a chemical process that introduces a hydroxyl group (-OH) into an organic compound. In biochemistry, hydroxylation reactions are often facilitated by enzymes called hydroxylases. Hydroxylation is the first step in the Consortium for Educational Communication oxidative degradation of organic compounds in air. It is extremely important in detoxification since hydroxylation converts lipophilic compounds into water-soluble (hydrophilic) products that are more readily excreted. Some drugs (e.g. steroids) are activated or deactivated by hydroxylation. Hypocotyls: The hypocotyl (short for “hypocotyledonous stem”,meaning “below seed leaf”) is the stem of a germinating seedling, found below the cotyledons (seed leaves) and above the radicle (root). Intercalary meristem A meristem located between non-dividing tissues, such as near the base of a blade of grass. Lactone ring: In chemistry, a lactone is a cyclic ester] which can be seen as the condensation product of an alcohol group -OH and a carboxylic acid group -COOH in the same molecule. It is characterized by a closed ring consisting of two or more carbon atoms, and a single endocyclic oxygen coupled with an adjacent ketone (typical ester type functional group). Long day plants: Describes plants that initiate flowers when day length is longer than their critical day length. M phase of mitosis: Mitosis is the process by which a eukaryotic cell separates the chromosomes in its cell nucleus into two identical sets, in two separate nuclei. It is generally followed immediately by cytokinesis, which divides the nuclei, cytoplasm, organelles and cell membrane into two cells containing roughly equal shares of these cellular components.Mitosis and cytokinesis together define the mitotic (M) phase of the cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell. This accounts for approximately 10% of the cell cycle. Microfibrils: A structure made up of several microfibrils wound together into a “cable;” provide the framework for the plant cell wall Membrane vesicles: In cell biology, a vesicle is a small bubble within a cell, and thus a type of organelle. Enclosed by lipid bilayer, vesicles can form naturally, for example, during endocytosis (protein absorption). Alternatively, they may be prepared artificially, when they are called liposomes. If there is only one phospholipid bilayer, they are called unilamellar vesicles; otherwise they are called multilamellar. The membrane enclosing the vesicle is similar to that of the plasma membrane, and vesicles can fuse with the plasma Consortium for Educational Communication membrane to release their contents outside of the cell. Vesicles can also fuse with other organelles within the cell Mevalonic acid pathway: Mevalonic acid (MVA) (derived from a contraction of dihydroxymethylvalerolactone) is a key organic compound in biochemistry. The anion of mevalonic acid, the predominant form in biological media, is known as mevalonate. This compound is of major pharmaceutical importance. Drugs, such as the statins, stop the production of mevalonate by inhibiting HMGCoA reductase. Mitosis Cell division that results in two daughter cells that are genetically identical to the parent cell. Mutation A random genetic variation that is passed on to offspring. Paclobutrazol: Paclobutrazol (PBZ) is a plant growth retardant and triazole fungicide. It is a known antagonist of the plant hormone gibberellin. It acts by inhibiting gibberellin biosynthesis, reducing internodial growth to give stouter stems, increasing root growth, causing early fruitset and increasing seedset in plants such as tomato[1] and pepper.[2] PBZ has also been shown to reduce frost sensitivity in plants. Phosphon D: Phosphon-D (tributyl-2, 4-dichlorobenzylphosphonium chloride), known as an inhibitor of gibberellin biosynthesis, enhances photosynthetic electron transport by up to 200%, with Fe(CN)63-and NADP+ being the electron acceptors Phosphoryl choline: Phosphorylcholine: is the hydrophilic polar head group of some phospholipids, which is composed of a negatively charged phosphate bonded to a small, positively charged choline group. Phosphorylcholine is part of plateletactivating factor; the phospholipid phosphatidylcholine as well as sphingomyelin, the only phospholipid of the membrane that is not built with a glycerol backbone. Phosphoryl choline citidyl transferase: This enzyme belongs to the family of transferases, specifically those transferring non-standard substituted phosphate groups. The systematic name of this enzyme class is CDP choline: 1,2-diacylglycerol cholinephosphotransferase. Phytochromes: A light-sensitive protein pigment involved in the Consortium for Educational Communication photoperiodic response Plant pathologists: Plant pathology (also phytopathology) is the scientific study of plant diseases caused by pathogens (infectious diseases) and environmental conditions (physiological factors). Proplastids: Plastids are major organelles found in the cells of plants and algae. They are the site of manufacture and storage of important chemical compounds used by the cell. Plastids often contain pigments used in photosynthesis, and the types of pigments present can change or determine the cell’s color. They possess a double-stranded DNA molecule, which is circular, like that of prokaryotes. Protease: A protease (also termed peptidase or proteinase) is any enzyme that conducts proteolysis, that is, begins protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain forming the protein. Quantitative analysis: in analytical chemistry, the measurements of quantities of substances produced in reactions rather than simply noting the nature of the reactions. Repressors: In molecular genetics, a repressor is a DNA-binding protein that regulates the expression of one or more genes by binding to the operator and blocking the attachment of RNA polymerase to the promoter, thus preventing transcription of the genes. This blocking of expression is called repression. Ribosomal RNA: Ribosomal ribonucleic acid (rRNA) is the RNA component of the ribosome, and is essential for protein synthesis in all living organisms. It comprises the predominant material within the ribosome, which is ca. 60% rRNA and 40% protein by weight. Ribosomes contain two major rRNAs and 50 or more proteins. Seed dormancy: A dormant seed is one that is unable to germinate in a specified period of time under a combination of environmental factors that are normally suitable for the germination of the nondormant seed.Dormancy is a mechanism to prevent germination during unsuitable ecological conditions, but the probability of seedling survival is low Signal transduction: Signal transduction occurs when an extracellular signalling, molecule activates a cell surface receptor. In turn, this receptor alters intracellular molecules creating a response. There are two stages in this process: Consortium for Educational Communication 1.A signalling molecule activates a specific receptor protein on the cell membrane. 2.A second messenger transmits the signal into the cell, eliciting a physiological response: • • • Transcriptional regulator: These transcription factors position RNA polymerase at the start of a protein-coding sequence and then release the polymerase to transcribe the mRNA. Activators enhance the interaction between RNA polymerase and a particular promoter, encouraging the expression of the gene. Activators do this by increasing the attraction of RNA polymerase for the promoter, through interactions with subunits of the RNA polymerase or indirectly by changing the structure of the DNA. Enhancers are sites on the DNA helix that are bound to by activators in order to loop the DNA bringing a specific promoter to the initiation complex. Ubiquitination: Ubiquitin is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. It directs proteins to compartments in the cell, including the proteasome which destroys and recycles proteins Xyloglucan endotransglycosylase: In enzymology, a is an enzyme that catalyzes the chemical reaction in which a beta-(14) bond in the backbone of a xyloglucan in broken; the xyloglucanyl segment is then transferred to the O4 of the non-reducing terminal glucose residue of either xyloglucan or an oligosaccharide thereof.