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Download Bacteria Notes - Sardis Secondary
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Kingdom Monera(Archaebacteria & Eubacteria) • • All bacteria are prokaryotes Characteristics: 1. No nucleus 2. No membrane bound organelles 3. Smaller & less ribosomes 4. Most are smaller than eukaryotes 5. Most are single-celled organisms Cell Structure & Function • Capsule • • • Cell Wall • • • Gives the cell shape Prevents osmosis from bursting the cell Cell Membrane • • • Sticky covering on the cell wall For protection and adherence Surrounds the cell Regulates transport Flagellum • Whip like protrusion for movement Cell Structure & Function • Pili • • Chromosome • • • Single DNA molecule Not contained within a nucleus Plasmid(s) • • hairlike structures for sticking to another bacteria during conjugation Genes located in small circular piece Ribosomes • Create protein necessary for life functions 1. Classification by Kingdom A. Kingdom Archaebacteria: • The Extremists • Harsh environments • Cell structures differ from other monerans B. Kingdom Eubacteria: • true or traditional bacteria 2. Classification by Energy Source A. Autotrophs: organisms that produce their own food i. Photoautotroph: uses energy from sun for food ii. Chemoautotroph(chemosy nthesizers): obtain energy from inorganic substances B. Heterotrophs: organisms that cannot produce their own food i. Photoheterotroph: uses sunlight for energy but obtains carbon from other organic source ii. Chemoheterotroph: obtain energy by using inorganic molecules and breaking them down C. Saprophytes or Decomposers • Obtain energy from breaking down dead organisms D. Parasites • Life in/on another organism host at the expense of the host but without killing • Pathogen: any disease causing bacteria 3. Classification by environment A. Archaebacteria 1. Methanogens • • • Produce methane Live in swamps, guts of cows & humans Used to treat sewage & purify water 2. Halophiles • Live in extremely salty places (eg. Dead Sea or Salt Lake) 3. Thermoacidophiles • • • Live in hot acidic H20 in hot springs Live in deep sea volcanic vents Some use inorganic compounds for energy (chemosynthetic) B. Eubacteria 1. Heterotrophic bacteria • • • • Live everywhere Survive on organic molecules Some are parasites Some are saprophytes 2. Photosynthetic Autotrophs • • • Cyanobacteria Perform plant-like photosynthesis Provide O2 for aquatic environments 3. Chemosynthetic Autotrophs • • Use inorganic compounds for energy Some convert N2 into N03 for plants 4. Classification by Gram Staining 1. Gram Positive Bacteria • Cell wall made of protein and sugar • Turn purple after gram staining 2. Gram Negative Bacteria • Extra layer of lipid outside of cell wall • Turn pink/red after gram staining • Require different types of antibiotics to treat infections 5. Classification by Shapes 1. Spherical (coccus) • Eg. Streptococcus and Pneumoncoccus 2. Rod-shaped (bacillus) • Eg. Escherichia coli (E. coli) 3. Spiral (spirillum) • Eg. Treponema pallidum (syphilis) Bacterial Shapes 6. Classification by Cellular Respiration • Process by which nutrients are broken down to provide energy A. Obligate aerobes • Need oxygen to live (eg. Tuberculosis) B. Obligate anaerobes • Killed by oxygen (eg. Clostridium tetani in deep wounds) C. Facultative anaerobes • Can use oxygen if it is available (eg. E. coli in human intestines) Reproduction 1. Binary Fission (asexual reproduction) • Method of reproduction for most bacteria • Occurs under normal conditions • Process: 1. DNA replicates (ie. doubles) 2. Cell divides 3. Result: 2 identical daughter cells 2. Conjugation (sexual reproduction) • DNA is exchanged between bacteria cells • Cell to cell contact using pili • Increases diversity 3. Transduction (sexual) • Use of viruses to transfer DNA between bacteria cells • Video clip 4. Transformation (sexual) • Bacteria cells that pick up DNA from dead bacteria cells Video clip Endospores • • • • Some bacteria can transform into dormant state called an Endospore A way to survive difficult conditions Involves the formation of a thick wall around itself They are able to resist: • • • heat drought radiation Diversity of Bacteria 1. 2 separate Kingdoms 2. Method of Obtaining Energy 3. Environment where they live 4. Cell Wall • Gram Positive or Negative 5. Shape (circle, rod, spiral) 6. Cellular Respiration 7. Colony Appearance • Colour • Shape (paired, clumps, chains) • Shininess Practical uses for Bacteria • Bacteria is both beneficial and essential for humans • E. coli, which is present in the colon, metabolizes fecal material and allows animals to absorb the minerals and Vitamin K that is produced. • Used in food production (cheese, yogurt) • Decomposers(recycling of nutrients, supplying O2) • Nitrogen fixing bacteria in plants(used for growth) • Antibiotic production Practical Uses for Bacteria Bacteria are also used in the process of producing recombinant DNA • DNA from another source is placed into the bacterial DNA • This creating a new useful strain • Utilizes bacteria to mass-produce proteins of specific interest like: • blood clotting factor • hormones • insulin