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PROBLEM SET Macromolecule Review Use the diagram on the following page while working through this activity. Part A. Answer these questions. Then use your answers to develop simple rules for identifying carbohydrates, lipids, proteins, and nucleic acids. 1. What is the approximate C:H:O ratio in each of the following types of macromolecules? (If there is no predictable ratio, write none.) Carbohydrates Lipids Proteins Nucleic acids 2. Which of the molecules listed above can often be composed of C, H, and O alone? 3. Which of the compounds can be identified by looking at the C:H:O ratios alone? 4. What other elements are commonly associated with each of these four types of macromolecules? Carbohydrates Always contain P Generally contain no P Always contain N Generally contain no N Frequently contain S Generally contain no S Lipids Proteins Nucleic acids 5. Functional groups can modify the properties of macromolecules. In the following table, indicate whether each of the following structures is polar or nonpolar and hydrophilic or hydrophobic. Which of these are found in proteins vs. lipids? Structure Polar or nonpolar Hydrophilic or hydrophobic Found in all proteins Found in many proteins Found in many lipids —OH (hydroxyl) —CH2— —COOH (carboxyl) —NH2 (amino) —SH (sulfhydryl) —PO4 (phosphate) 6. You are studying a type of virus. Viruses are tiny particles that consist of RNA inside a protein “shell.” You want to use a radioactive tracer that will label only the protein (but not the RNA) of the virus. Which of the following would you use? a. Radioactive P b. Radioactive N c. Radioactive S d. Radioactive C 7. Closely related macromolecules often have many characteristics in common. For example, they share many of the same chemical elements and functional groups. Therefore, to separate or distinguish closely related molecules, you need to determine how they differ and then target or label that difference. What makes RNA different from DNA? If you wanted to use a radioactive or fluorescent tag to label only the RNA in a cell and not the DNA, what compound(s) could you label that are specific to RNA? If you wanted to label only the DNA, what compound(s) could you label? 8. Based on your answers to questions 1-7, what simple rule(s) can you use to identify the following macromolecules? Carbohydrates Lipids Proteins Nucleic acids DNA vs. RNA Part B. Carbohydrate, lipid, protein, or nucleic acid? Name that structure! 1. Based on the rules you developed in Part A, identify the following compounds as carbohydrates, lipids, amino acids, proteins, or nucleic acids. In addition, indicate whether each is likely to be polar or nonpolar, hydrophilic or hydrophobic. 2. A student, Mary, is given four samples and told they are lysine (an amino acid), lactose (a disaccharide), insulin (a protein), and RNA. The samples are in test tubes marked 1, 2, 3, and 4, but Mary doesn’t know which compound is in which tube. She is instructed to identify the contents of each tube. • In her first test, she tries to hydrolyze a portion of the contents of each tube. Hydrolysis occurs in all tubes except tube 3. • In Mary’s next test, she finds that tubes 1, 2, and 3 are positive for nitrogen, but only tube 2 gives a positive result for sulfur. • The last test Mary performs shows that the compound in tube 1 contains a high percentage of phosphate. 3. Based on these data, fill in the following table and explain your answers. Tube number Contents Explanation 1 2 3 4 4. Use your understanding of the chemical characteristics of carbohydrates, proteins, and lipids in living organisms to predict the outcome of the following four experiments. Be sure to explain your reasoning. Experiment A: You stir 10 g of glucose and 10 ml of phospholipids in a 500-ml beaker that contains 200 ml of distilled water. Draw a diagram to show where and how the glucose and phospholipids would be distributed after you let the mixture settle for about 30 minutes. Experiment B: You repeat Experiment A again, but this time you stir 10 g of glucose and 10 ml of phospholipids in a different 500-ml beaker that contains 200 ml of distilled water and 100 ml of oil. Draw a diagram to show where and how the glucose, phospholipids, and oil would be distributed after you let the solution settle for about 30 minutes. 5. Complete the table below by listing the appropriate monomers, polymers and examples of the four major types of molecules found in cells. The answers can be found in the word list below. Each answer is only used once. • polypeptide • monosaccharide • enzyme • amino acid • polysaccharide • nucleic acid • fatty acid • antibody • starch • nucleotide • glucose • ATP • triglyceride • oligosaccharide • glycerol • disaccharide • phospholipid • lactose • DNA Types of molecule Lipids Proteins Carbohydrates Nucleic acids "Monomers" 1. ____________________ 2. ____________________ 1. ____________________ 1. ____________________ 1. ____________________ Polymers Not applicable 2. ____________________ Examples: 1. ____________________ 2. ____________________ 1. ____________________ 2. ____________________ 1. ____________________ 1. ____________________ 2. ____________________ 2. ____________________ 3. ____________________ 3. ____________________ 1. ____________________ 1. ____________________ 2. ____________________