Download Human Primary Cell cDNA

Page 1 of 1
Figure 1: cDNA Sample
5858 Horton Street, Suite 360
Emeryville, CA 94608
Phone: 510-450-3900
Fax: 510-450-3903
www.allcells.com
Human Primary Cell cDNA
Shipping and Storage
AllCells cDNA products are aliquoted at a base concentration of 1 PCR
reaction/2µL DEPC-treated H2O and shipped on dry ice. This product is
stable at –80°C for 8 to 12 months. Avoid repeated freezing or thawing.
This cDNA product can be stored at –20°C for less than a month. This
product should not be stored in defrost freezer.
CAUTION
The products purchased from AllCells, LLC are “For In Vitro
Research Use Only.” In compliance with U.S. Food and Drug
Administration (FDA) Regulations, these products are intended
neither for use in clinical diagnostic procedures nor in
therapeutic procedures.
These products must be:
1. Treated as potentially contaminated biological
specimens even if available serological reports are
negative.
2. Handled by establishing or following appropriate
safety control procedures to ensure the safety of
using these products.
Product Description
AllCells human primary cell cDNA is synthesized from highly purified
RNA, which is derived from high purity, primary hematopoietic cells.
These cells are isolated from human blood, bone marrow, or umbilical
cord blood using immunomagnetic MicroBead labeling procedures.
Application
The above image is an Agilent 2100 Bioanalyzer electropherogram image of
a sample of cDNA.
Frequently Asked Questions
Q: Is the cDNA product double-strand or single-strand?
A: AllCells cDNA products are single-strand. The original RNA template
used to create the cDNA is digested using RNase H.
Q: Is the cDNA product stable in DEPC-treated H2O?
A: Our cDNA product is stable for 8 to 12 months in DEPC-treated H2O.
While the single-strand cDNA product is relatively less stable than doublestrand cDNA, it is still significantly more stable than RNA.
References
Human primary cell cDNA is developed to meet researchers’ needs in
gene expression, cloning studies, and other experiments in various
molecular biology fields.
Bayasal, Bora E. et al. (2007). A recurrent stop-codon mutation in succinate
dehydrogenase, subunit B gene in normal peripheral blood and childhood Tcell acute leukemia. PLOS ONE 2(5): e436.
cDNA Synthesis
Related Products
The single-strand cDNA is designed for studying gene expression or
cloning genes by PCR amplification for various types of purified human
cells. RNA is purified from the cells by using an optimized procedure to
ensure that the RNA is of highest quality and free of genomic DNA
contamination. Full-length high fidelity cDNA is synthesized by a reverse
transcriptase from RNA primed with oligo-d(T). The original RNA
templates are removed from cDNA by using RNase H.
cDNA Purity
Human cDNA products can be tested using PCR primers from a list of
human housekeeping genes, such as beta-actin and GAPDH. AllCells
uses a pair of beta acton primers that are intron-crossing. The PCR
product is examined by the Agilent BioAnalyzer 2100 for correct product
size. In addition, the starting cDNA material is run through the Agilent
Bioanalyzer 2100 for integrity analysis.
Customers who have ordered AllCells cDNA are also interested in:

Total RNA: Larger RNA fragments (>200nt).

Comprehensive RNA: AllCells Comprehensive RNA includes a
combination of Total RNA (>200nt) and micro RNA (19-200nt).

Lysates: AllCells fresh cells are spun down and lysed with RLT or
Trizol.
For more information on AllCells, LLC products please contact an AllCells
Customer Service Representative at the following:
Phone: 510-450-3900; Toll-Free: 1-888-535-3444; Fax: 510-450-3903
Email: [email protected]; Web: http://www.allcells.com
5000-FRM-510. Rev. no. 002
January 2009
© 2008 AllCells, LLC. All rights reserved.