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Download The accompanying Excel spread sheet contains four columns of
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The accompanying Excel spread sheet contains four columns of data, presented as expression ratios. The first is a control with the Cy3-labeled wild-type (CU1065) cDNA cohybridized with Cy5-labeled wild-type cDNA. The following three slides are cohybridization of cDNA prepared from wild-type (CU1065) versus cDNA prepared from the isogenic sigW mutant strain. The data are sorted in terms of increasing ratios based on the first experimental data set. The genes near the top in this arrangement are those most decreased in expression in the sigW mutant, and therefore most likely to be targets for sigW-dependent transcription. Genes near the bottom of the spreadsheet, for which valid ratios were obtained, are those that are up-regulated in the sigW mutant. Many of these genes belong to the sigma-B-dependent general stress response. Blanks indicate that no ratio was calculated as the signals detected for that gene were at or near background. Ratios calculated when one signal (either the Cy3 or the Cy5 channel) was near background are flagged in the columns labeled “Fluor. w/in 1SD of Background.” These ratios should be considered approximate and are not included when computing average ratios of induction. Note that these arrays contain ~90% of the annotated B. subtilis ORFs as described in Methods and Materials. Note that individual gene values can be obtained using the “Find” function of Microsoft Excel and the data can easily be re-sorted to generatean alphabetic list if desired. J. Helmann 1-21-02