Download GE Nova Video Questions

The following questions are based on the video “Genetic
Engineering” available from Phillip & Harris catalogues.
Worksheet on Novo
Note: This video is 15 minutes in total. The answers to the worksheet are
found between 6.55 minutes and 10 minutes approx.
1. Plasmids are used in genetic engineering (g.e.).Name a unicellular
organism that contains plasmids?
2. Why are plasmids suitable for use in g.e.?
3. Specific enzymes, called restriction enzymes, are used in g.e.
experiments. What is their purpose?
4. There are a number of stages involved in doing simple g.e.
experiments. Fill in the blanks below to describe these stages:
(i)
The plasmid is isolated and ……………………… with a
restriction enzyme.
(ii)
The DNA in the chromosome of the donor is cut with the
same restriction ………………… so that the donor ………..
can be isolated.
(iii)
The donor gene and split plasmid are …………………
together.
(iv)
The plasmid with the donor gene is …………………… to a
recipient cell.
Kerry Teacher Design Team In association with The Biology Support Service, The Education Centre, Tralee
Teachers Answer Sheet
1. Bacteria
2. Plasmids can multiply at the same rate as the DNA of the cell
Plasmids are present in larger numbers than the chromosomes in cells
The size of plasmids make them easier to handle than chromosomal
DNA.
3. To cut DNA.
4. (i) cut
(ii) enzyme, gene
(iii) mixed
(iv) transferred
5. Insulin.
Kerry Teacher Design Team In association with The Biology Support Service, The Education Centre, Tralee