Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Paper Plasmid Lab Period 3 Notes.notebook
Document related concepts
Gene nomenclature wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
Gene expression profiling wikipedia , lookup
Genetically modified crops wikipedia , lookup
Genetically modified food wikipedia , lookup
Gene regulatory network wikipedia , lookup
Endogenous retrovirus wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Genetically modified organism wikipedia , lookup
Biotechnology wikipedia , lookup
Restriction enzyme wikipedia , lookup
Gene prediction wikipedia , lookup
Gene therapy wikipedia , lookup
Community fingerprinting wikipedia , lookup
Molecular cloning wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Genome editing wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
History of biotechnology wikipedia , lookup
DNA vaccination wikipedia , lookup
Genomic library wikipedia , lookup
Designer baby wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Genetic engineering wikipedia , lookup
Transcript
Paper Plasmid Lab Period 3 Notes.notebook March 03, 2015 Do Now 1. What would we use to cut a gene? 2. What is the role of the plasmid in genetic engineering? HMWK: Lab Quiz next lab day Objectives 1. SWBAT explain the function of a restriction enzyme. 2. SWBAT model the process of making a recombinant DNA plasmid from a desired gene and the plasmid. 1 Paper Plasmid Lab Period 3 Notes.notebook March 03, 2015 Bacteria cells have plasmids, much smaller than bacterial chromosome! Things to remember about plasmids: 1. During replication, the plasmids must also replicate. 2. Plasmids can be removed and harvested from the cell. 3. Plasmids are used in genetic engineering and biotechnology. Plasmid structure: 1. DNA is antiparallel 2. Held together by hydrogen bonds 3. Plasmids have a small # of genes 4. Genetically modified: 2 Paper Plasmid Lab Period 3 Notes.notebook March 03, 2015 Genetic Engineering 1. Goal: place desired gene into a plasmid 2. Cut both gene of interest and plasmid with restriction enzymes 3. Leave sticky ends that will adhere to each other 4. Hydrogen bonds will form between sticky ends 5. Ligase will create phosphodiester bonds between the bases. **** Recombinant DNA****** 3
