Download Paper Plasmid Lab Period 3 Notes.notebook

Paper Plasmid Lab Period 3 Notes.notebook
March 03, 2015
Do Now
1. What would we use to cut a gene?
2. What is the role of the plasmid in genetic engineering?
HMWK: Lab Quiz ­ next lab day
Objectives
1. SWBAT explain the function of a restriction enzyme.
2. SWBAT model the process of making a recombinant DNA plasmid from a desired gene and the plasmid.
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Paper Plasmid Lab Period 3 Notes.notebook
March 03, 2015
Bacteria cells have plasmids, much smaller than bacterial chromosome!
Things to remember about plasmids:
1. During replication, the plasmids must also replicate.
2. Plasmids can be removed and harvested from the cell.
3. Plasmids are used in genetic engineering and biotechnology.
Plasmid structure:
1. DNA is antiparallel
2. Held together by hydrogen bonds
3. Plasmids have a small # of genes
4. Genetically modified:
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Paper Plasmid Lab Period 3 Notes.notebook
March 03, 2015
Genetic Engineering
1. Goal: place desired gene into a plasmid
2. Cut both gene of interest and plasmid with restriction enzymes
3. Leave sticky ends that will adhere to each other
4. Hydrogen bonds will form between sticky ends
5. Ligase will create phosphodiester bonds between the bases.
**** Recombinant DNA******
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