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Transcript
Recombinant DNA and Plasmids Guiding Questions. 1. What is a plasmid? Circular Extrachromosomal Able to replicate About one thousand times smaller than the bacterial chromosome NOT A small section of bacterial DNA A strand of DNA for resistance 2. Why do bacteria carry them? Plasmids contain useful genes Plasmids can be transferred between bacteria more easily than whole chromosomes. NOT So proteins can multiply on the plasmid So they can provide resistance Plasmids produce more bacteria as they are cloned? To protect from resistance So plasmids can make protein They are restriction proteins 3. Why cant we take genes of interest to us (GFP, Insulin, etc.) and simply circularize them and transfer them into bacteria? Antibiotic resistance genes give us a way to select for a transformation event (find the bacteria with the plasmid). The plasmids carry an origin of replication that is a start point for DNA polymerase. Without it a plasmid would dilute out of the population. NOT Because it doesn’t have the plasmid in it Didn’t contain any bacterial DNA The bacteria wouldn’t recognize the gene as necessary Because we need more? Why are plasmids useful to modern DNA science? They give us a way to move genes of interest into bacteria so they can express the protein in large amounts. [They are vectors] NOT They help sickness with antibiotics (they carry antibiotics) Help make medicines to harmful bacteria Create antibiotics to combat bacteria Plasmids provide resistance to infections
