notes for mondays lab
... 2. Proteinase K: an enzyme that catalyzes the breakdown of cellular proteins by splitting them into smaller peptides and amino acids 3. Buffer AL: a cell lysis solution that breaks open cell and nuclear membranes 4. Ethanol: used to precipitate DNA from the extracted material 5. Buffer AW1 and AW2: ...
... 2. Proteinase K: an enzyme that catalyzes the breakdown of cellular proteins by splitting them into smaller peptides and amino acids 3. Buffer AL: a cell lysis solution that breaks open cell and nuclear membranes 4. Ethanol: used to precipitate DNA from the extracted material 5. Buffer AW1 and AW2: ...
Genetic Engineering (and other cool molecular biology techniques)
... • Specific sequence of DNA is amplified (copied many times) • Requires: – DNA template (contains your gene of interest) – Tac polymerase (a DNA polymerase that can work at high temperatures) – Nucleotides (to synthesize new DNA) – Primers (specific to the gene of interest) ...
... • Specific sequence of DNA is amplified (copied many times) • Requires: – DNA template (contains your gene of interest) – Tac polymerase (a DNA polymerase that can work at high temperatures) – Nucleotides (to synthesize new DNA) – Primers (specific to the gene of interest) ...
Chapter 5
... Recombinant DNA technology (Gene cloning, molecular cloning, genetic engineering) Methodology for transferring genetic information (genes) from one organism to another • Characterization of the genes • Large production of proteins • Mutants ...
... Recombinant DNA technology (Gene cloning, molecular cloning, genetic engineering) Methodology for transferring genetic information (genes) from one organism to another • Characterization of the genes • Large production of proteins • Mutants ...
Me oh Mi!
... Name 3 things that can be used as DNA evidence that were used in the movie GATTACA ...
... Name 3 things that can be used as DNA evidence that were used in the movie GATTACA ...
The Wild World of Biotechnology!! Applications Genetic
... Isolate the gene of interest (e.g. the gene for insulin) using restriction enzymes This is a hit or miss process that requires a great deal of luck to be successful Restriction enzymes generally cut at palindromic DNA sequences ...
... Isolate the gene of interest (e.g. the gene for insulin) using restriction enzymes This is a hit or miss process that requires a great deal of luck to be successful Restriction enzymes generally cut at palindromic DNA sequences ...
Virtual DNA Lab
... 4. Examine the picture. Which type of bonds hold the DNA bases together? ____________________ 5. What molecule do genes create? ____________________________________________________ What is a Gene? 6. Why are genes called the instruction manual for our body? _______________________________ __________ ...
... 4. Examine the picture. Which type of bonds hold the DNA bases together? ____________________ 5. What molecule do genes create? ____________________________________________________ What is a Gene? 6. Why are genes called the instruction manual for our body? _______________________________ __________ ...
Transformation Lab
... cleaves the disaccharide sugar lactose into galactose and glucose. However, the enzyme will also cleave many other molecules that contain structures resembling the galactoseglucose link. Researchers have developed several chemicals that, when cleaved by beta-galactosidase, produce colored products. ...
... cleaves the disaccharide sugar lactose into galactose and glucose. However, the enzyme will also cleave many other molecules that contain structures resembling the galactoseglucose link. Researchers have developed several chemicals that, when cleaved by beta-galactosidase, produce colored products. ...
MolecularBiology1APLab6
... are collected or exchanged w/ other bacteria • Contain nonsense information • Sometimes contain useful information like antibiotic resistance ...
... are collected or exchanged w/ other bacteria • Contain nonsense information • Sometimes contain useful information like antibiotic resistance ...
13-2 Manipulating DNA
... 5) Used to locate and identify a particular genes or used to compare individuals. Knowing the sequence of an organism’s DNA allows researchers to study specific genes, to compare them with the genes of other organisms, and to try to discover the functions of different genes and gene combinations. ...
... 5) Used to locate and identify a particular genes or used to compare individuals. Knowing the sequence of an organism’s DNA allows researchers to study specific genes, to compare them with the genes of other organisms, and to try to discover the functions of different genes and gene combinations. ...
2 Types of Selective Breeding
... For thousands of years people have tried to produce __________________________ with desirable traits _________ methods that people use to develop organisms with desirable traits: 1) Selective Breeding – a process of selecting a few organisms with _______________ to serve as parents of the __________ ...
... For thousands of years people have tried to produce __________________________ with desirable traits _________ methods that people use to develop organisms with desirable traits: 1) Selective Breeding – a process of selecting a few organisms with _______________ to serve as parents of the __________ ...
(1) End labelling
... Primer extension • * This labelling technique uses random oligonucleotides (usually hexadeoxyribonucleotide moleculessequences of six deoxynucleotides) to primer synthesis of a DNA strand by DNA polymerase. • * The DNA to be labelled is denaturated by heating, and the oligonucleotide primer annealed ...
... Primer extension • * This labelling technique uses random oligonucleotides (usually hexadeoxyribonucleotide moleculessequences of six deoxynucleotides) to primer synthesis of a DNA strand by DNA polymerase. • * The DNA to be labelled is denaturated by heating, and the oligonucleotide primer annealed ...
WS 12 - Department of Chemistry | Oregon State University
... Why is dATP one of the four precursors of DNA, but dAMP is not? ...
... Why is dATP one of the four precursors of DNA, but dAMP is not? ...
The Genetic Revolution
... found in bacterial cells that carry extrachromosomal genes • b. plasmids are circular, doublestranded DNA molecules capable of autonomous replication within living cells. • c. although not essential for the survival of their host, they may encode a wide variety of genetic determinants that increase ...
... found in bacterial cells that carry extrachromosomal genes • b. plasmids are circular, doublestranded DNA molecules capable of autonomous replication within living cells. • c. although not essential for the survival of their host, they may encode a wide variety of genetic determinants that increase ...
Application Sheet: DNA - NETZSCH Thermal Analysis
... APPLICATION SHEET ORGANICS – PHARMACEUTICALS ...
... APPLICATION SHEET ORGANICS – PHARMACEUTICALS ...
DNA Technology
... DNA Technology This involves Restriction enzymes: Enzymes that recognize a DNA sequence (usually a palindrome) and cut DNA at that sequence. ( p. 398 in textbook) Two major types of DNA Technology A. Gene Splicing: DNA from one organism is spliced into another organism (bacteria) (see p. 397-399) (R ...
... DNA Technology This involves Restriction enzymes: Enzymes that recognize a DNA sequence (usually a palindrome) and cut DNA at that sequence. ( p. 398 in textbook) Two major types of DNA Technology A. Gene Splicing: DNA from one organism is spliced into another organism (bacteria) (see p. 397-399) (R ...
Genetic Engineering
... • Separates DNA fragments based on size • The smaller the fragment the farther it will move • Can compare DNA from individuals ...
... • Separates DNA fragments based on size • The smaller the fragment the farther it will move • Can compare DNA from individuals ...
Chapter 24 Applied Genetics I. Plant and animal
... 1. Crossing of two organisms with the same or similar sets of genes 2. Leads to purebred organisms 3. Able to pass on desirable traits 4. May cause susceptibility to certain diseases Genetic engineering A. Recombinant DNA 1. Joining parts of an organism’s DNA to the DNA of another organism 2. Transf ...
... 1. Crossing of two organisms with the same or similar sets of genes 2. Leads to purebred organisms 3. Able to pass on desirable traits 4. May cause susceptibility to certain diseases Genetic engineering A. Recombinant DNA 1. Joining parts of an organism’s DNA to the DNA of another organism 2. Transf ...
VII. DNA/ GENES/ AND GENETICS • Describe the relationship
... What mechanism do cells use to turn genes on and off? Give examples of emerging biotechnologies. What modern technologies are currently being implemented to determine evolutionary relationships among species? How are viruses used to treat disease? How can over exposure to sunlight cause skin cancer? ...
... What mechanism do cells use to turn genes on and off? Give examples of emerging biotechnologies. What modern technologies are currently being implemented to determine evolutionary relationships among species? How are viruses used to treat disease? How can over exposure to sunlight cause skin cancer? ...
Chapter 10 Section 3 Notes Answer Key
... I. DNA A. DNA- a chemical that contains information an organism needs to grow and function 1. Watson and Crick made and accurate model of DNA in 1953 2. The structure of DNA is similar to a twisted ladder. a. The sides of the ladder are made up of sugarphosphate molecules. b. The rungs of the ladder ...
... I. DNA A. DNA- a chemical that contains information an organism needs to grow and function 1. Watson and Crick made and accurate model of DNA in 1953 2. The structure of DNA is similar to a twisted ladder. a. The sides of the ladder are made up of sugarphosphate molecules. b. The rungs of the ladder ...
2.5.4. DNA Revision Qs
... (b) the production of an enzyme _____________________________________ (c) the ability to play a musical instrument _____________________________________ (d) the ability to form a blood clot _____________________________________ (e) the ability to read _____________________________________ ...
... (b) the production of an enzyme _____________________________________ (c) the ability to play a musical instrument _____________________________________ (d) the ability to form a blood clot _____________________________________ (e) the ability to read _____________________________________ ...
genetics - Lemon Bay High School
... He noticed patterns among the generations of plants when they were cross-pollinated by hand and then allowed to fertilize naturally. ...
... He noticed patterns among the generations of plants when they were cross-pollinated by hand and then allowed to fertilize naturally. ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.