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Add to wolbachia notes!MONDAY IS A BIG LAB! BE READY! DNA EXTRACTION REAGENTS 1. Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration 2. Proteinase K: an enzyme that catalyzes the breakdown of cellular proteins by splitting them into smaller peptides and amino acids 3. Buffer AL: a cell lysis solution that breaks open cell and nuclear membranes 4. Ethanol: used to precipitate DNA from the extracted material 5. Buffer AW1 and AW2: solutions that wash the DNA attached in the column membrane of contaminants 6. Buffer AE: a solution that elutes the DNA from the membrane and allows stable storage of DNA for years in the refrigerator or freezer MONDAY IS A BIG LAB!!!! BE READY!!! WOLBACHIA PCR We are amplifying part of the 16S ribosomal RNA gene that is unique to wolbachia. It is 438 bp. We are also amplifying a portion of the gene for cytochrome c oxidase. It is 650 bp. This is a gene specific to animals, not bacteria. Why would we do this?