Download Feb 21 Bacteria, DNA Technology, and Cell Communication

Bacteria Genetics, DNA Technology, and Cell Communication
Feb 21
Review
Plasmids
Conjugation
Hfr
Viral structure and types
Single vs. double recombination
Body
Transposons
Operons
Negative vs. positive regulation (repressors vs. enhancers)
Eukaryotic gene regulation—transcription factors
Difference btw prokaryote and eukaryote gene regulation
DNA technology
DNA is redundant main differences due to gene expression
Restriction enzymes, sticky ends, DNA ligase (endonuclease)
Palandromic sequence
DNA cloning
Gene Therapy 20.16
Pharm animals
Transgenic organisms
Cell Communication
Yeast mating
Local communication-gap junctions plasmodesmata, cell-cell recognistion
Local signaling- paracrine, synaptic (secreting cell target cell)
Long distance communication in the body hormonal signaling
Signal transduction- reception, transduction, response
Steroid hormones
G-protein-linked plasma membrane receptors
Plasma membrane tyrosine kinases
Plasma membrane ion-gated channel receptors
Signal transduction cascades
Second messengers
Signal amplification
Cell-Specific Responses to same signal
End
Describe the difference between steroid and G-protein linked signal transduction.
How does one signal molecule create a large response in the cell?
What is the purpose of second messengers?
How do cells communicate?
What vectors are used for gene therapy?
What are the three steps of signal transduction?
How do restriction enzymes incorporate new genetic material?