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Download Feb 21 Bacteria, DNA Technology, and Cell Communication
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Bacteria Genetics, DNA Technology, and Cell Communication Feb 21 Review Plasmids Conjugation Hfr Viral structure and types Single vs. double recombination Body Transposons Operons Negative vs. positive regulation (repressors vs. enhancers) Eukaryotic gene regulation—transcription factors Difference btw prokaryote and eukaryote gene regulation DNA technology DNA is redundant main differences due to gene expression Restriction enzymes, sticky ends, DNA ligase (endonuclease) Palandromic sequence DNA cloning Gene Therapy 20.16 Pharm animals Transgenic organisms Cell Communication Yeast mating Local communication-gap junctions plasmodesmata, cell-cell recognistion Local signaling- paracrine, synaptic (secreting cell target cell) Long distance communication in the body hormonal signaling Signal transduction- reception, transduction, response Steroid hormones G-protein-linked plasma membrane receptors Plasma membrane tyrosine kinases Plasma membrane ion-gated channel receptors Signal transduction cascades Second messengers Signal amplification Cell-Specific Responses to same signal End Describe the difference between steroid and G-protein linked signal transduction. How does one signal molecule create a large response in the cell? What is the purpose of second messengers? How do cells communicate? What vectors are used for gene therapy? What are the three steps of signal transduction? How do restriction enzymes incorporate new genetic material?