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GENETICS College Board Performance Objectives Explain how linked genes tend to be inherited together. Describe how independent assortment of chromosomes and crossing over produce genetic recombinants. Explain how geneticists use recombination data to map a chromosome's genetic loci. Discuss how mutations can be neutral, harmful, or even beneficial, and give examples. Solve genetics problems involving dominance, incomplete dominance, codominance and multiple alleles, There are no test questions about epistasis. Compare sex-linked inheritance with autosomal inheritance. List three examples of sex-linked traits and perform Punnett Squares for these examples. Explain how the structures of nucleic acids relate to their function of storage of information. Explain how the structures of nucleic acids relate to their functions of protein synthesis. Discuss the contributions of each in determining the structure and function of DNA: Watson, Crick, Franklin, Wilkins Avery-MacLeod-McCarty Experiments Hershey- Chase Experiment Meselson-Stahl Experiment Describe transcription and translation and relate each to possible mutations. Explain the structure and function of viruses. Explain the major steps in viral reproduction. Explain how viruses transfer genetic material between cells Describe the structure and function the lac and tryp operons. Explain how genetic information is organized in the eukaryotic chromosome and how the organization contributes to both continuity and variability in the genetic information. Explain some mechanisms by which gene expression is regulated in prokaryotic and eukaryotic genomes. Explain current recombinant technologies. Explain some practical applications of nucleic acid technology. Explain the legal and ethical problems that may arise from technology applications. 1 College Board Lab Objectives: Explain the principles of bacterial transformation and conditions under which cells can be transformed. Explain how a plasmid can be engineered to include a piece of foreign DNA. Explain how plasmid vectors are used to transfer genes. Explain how antibiotic resistance is transferred between cells. Explain how restriction endonucleases function. Explain the importance of restriction enzymes to genetic engineering experiments. Explain the use of plasmids as vectors to transform bacteria with a gene for antibiotic resistance in a controlled experiment. Demonstrate how restrictions enzymes are used in genetic engineering. Describe the biological process of transformation in bacteria. Calculate transformation efficiency. Use multiple experimental controls. Design a procedure to select positively for antibiotic resistant transformed cells. 2