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Name_________________________________ Biology CELL DIVERSITY VIEWING LAB DIRECTIONS: For each of the cells listed below, obtain or prepare (wet-mount or stained) a slide for each. Locate it under the microscope and draw & label what you see (as per the instructions). Then use your drawings (and your knowledge of cells) to answer the analysis questions at the end of the lab. NOTE: this meets/satisfies: Learner Outcome 4.01.1 (and GSEs LS1 (9-11)- 1a, 1b, and 1c) The Goals of this lab are to : 1. compare and contrast as well as explain relationships amongst specialized cells and structures/organelles within eukaryotic and prokaryotic cells 2. compare and contrast multi-celled organisms vs uni-celled organisms Do this first! PREDICTION(S): Predict differences you will see amongst eukaryotic cells and multi-celled organisms and uni-celled organisms: ____________________________________________________________________ ____________________________________________________________________ ___________________________________________________________________ Human Cheek Cell PLANT or ANIMAL CELL? MULTI-CELLED or UNI-CELLED? 1. Use a toothpick to scrape the inside of your cheek and then smear the cells on the slide. 2. Stain this sample using methylene blue. 3. Place a cover slip over the specimen and locate these cells Circle P or A 4. In the space below, draw and label the following: & MC or UC a. Cell membrane b. Nucleus c. Cytoplasm Magnification: ____________ Elodea species Cell PLANT or ANIMAL CELL? MULTI-CELLED or UNI-CELLED? 1. Use tweezers to tear off a small leaflet from the Elodea plant in the front of the room. 2. Place a small drop of water on the leaflet and cover it with a coverslip 3. In the space below please drawn and label: a. Chloroplasts b. Cell wall c. Vacuole d. Cytoplasm Magnification: ____________ Onion cell PLANT or ANIMAL CELL? MULTI-CELLED or UNI-CELLED? 1. Obtain a VERY THIN section of inner onion skin (HINT: think dead skin from a sunburn!!) 2. Place a flattened piece on a slide and stain it with 1 drop of iodine solution. 3. Cover it with a coverslip 4. In the space below please draw and label the following: a. Cell wall b. Cytoplasm c. Nucleus d. Nucleolus Magnification: ____________ Amoeba proteus slide PLANT or ANIMAL CELL? MULTI-CELLED or UNI-CELLED? 1. Obtain a prepared/fixed slide of an Amoeba. This is an aquatic, single-celled organism that lives in water. 2. Observe under the microscope 3. In the space below please draw and label the following: a. Nucleus b. Cytoplasm c. Cell membrane d. Vacuoles Magnification: ____________ Rat Liver Cell (using Transmission Electron Microscope PLANT or ANIMAL CELL? MULTI-CELLED or UNI-CELLED? Observe the TEM image of rat liver cell(s) and sketch it in the space below. Label as many organelles/structures as you can (at least 5). HINT: remember that this is magnified much more than what you would see in a normal compound light microscope. Be careful!! Magnification __________ Analysis Questions 1. Restate the “problem”/goals of this lab: 2. Of the specimens you viewed, which were animal cells? Which were plant cells? How do you know? Explain briefly. 3. Were there differences between the single celled and multi-celled organisms? How are they different? If you cannot see any differences at the cellular level, what about also at the organism level? HINT: think in terms of requirements and designs. Remember: “Form Fits Function”. Explain. 4. Using your answer from the above question (#3), explain some differences how a human (multicelled organism) and an Amoeba (single-celled organism) complete the following tasks: a. Obtain and digest food b. move around environment c. reproduce 5. Please list at least THREE different organelles/structures that you were UNABLE to view in this lab (that you feel you should have been able to see). Why could you not see them? Explain briefly. 6. Different parts of your body/cells will have different amounts of certain organelles. For each of the following, please list ONE organelle that you think you would find in abundance (find a lot of) and WHY you feel this way: a. Muscle cells from your leg: b. cells from inside of a cactus c. cells from your liver (HINT: think of your liver’s function/job) d. white blood cells that make up part of your immune system