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Biology Final Study Guide Last page questions due Monday, Dec. 15 This guide is NOT comprehensive! There may be material on your final exam that is not mentioned here! The first unit of the course discussed the basic tools and concepts needed to perform investigations in biology. Observation/Inference Observations are units of information that can be perceived with the senses. They are categorized as qualitative or quantitative. Inferences are conclusions based on observations. • The woman is sick. • The woman will skip work today. • The woman is cold. Chapter 1: Intro to Biology (p. 4-9, 15-28) Scientific Method Observations vs. Inferences Variables (Independent, Dependent, Control) Microscope Function Units of Measurement Characteristics of Living Things Biological Levels of Organization • The woman is on the telephone. • The woman has a fever. • The woman is holding a cup. Of the six statements above, only two are true observations! The rest are inferences drawn from the situation. The Scientific Method a systematic process by which scientists predict and deduce information. Observation, Hypothesis, Data Collection and Analysis are critical elements of the process. Experiments must be designed in ways that can be repeated by others. Variables are independent (deliberately changed by the experimenter), dependent (observed and measured), and controlled (kept constant so as not to interfere with the test). Smithers thinks that a special juice will increase the productivity of workers. He creates two groups of 50 workers each and assigns each group the same task (to staple a set of papers). Group A is given the special juice to drink while they work. Group B is not given the special juice. After an hour, Smithers counts how many stacks of papers each group has made. Group A made 1,587 stacks, Group B made 2,113 stacks. What are the independent and dependent variables? What variables should be controlled? Characteristics of Living Things (from textbook p. 16) Living things: • are made up of cells • reproduce • are based on a universal genetic code • grow and develop • obtain and use materials and energy • respond to stimuli • maintain a stable internal environment (via homeostasis) • change over time Levels of Organization the categories in which life can be studied: biosphere, ecosystem, community, population, organism, organ system, organ, tissue, cell, molecule For each level of organization, write a sentence to describe it. (Ex. a population is a group of organisms of the same species) Chapter 2: Biochemistry (p.34-53) Periodic Table of the Elements Subatomic Particles Atomic Bonding Ions and Isotopes Properties of Water Acid/Base/Buffer Chemistry Macromolecules Enzyme Activity The second unit addressed the principles of biochemistry. First and foremost, you must know how to read the periodic table! What are the names and characteristics of the three primary subatomic particles? Indicate their masses, charges and locations. Water is a molecule with special properties because of its molecular composition. H2O is a polar molecule because it has a positive side and a negative side despite having a net neutral charge. Draw a diagram of an H2O molecule including its electrons to show what makes it polar. Water’s polarity allows it to create hydrogen bonds which attract water molecules to each other. The cohesion among water molecules allows them to have high surface tension and high specific heat. Water is often found in mixtures which are categorized as solutions or suspensions. In solution, water is almost always the solvent, while a substance mixed into it (and in lower volume) is its solute. Acids and bases are solutions in which the ratio of hydrogen ion (H+) to hydroxide ion (OH-) is unequal. Pure water has a pH of 7, substances like stomach acid and lemon juice have pH values below 7 and alkaline substances like soap and bleach have pH values above 7. What are the physical characteristics of acids and bases? Buffers are resistant to changes in pH. The bicarbonate/carbonic acid system in human blood works as a buffer to prevent the blood pH from changing dramatically when we consume foods with extreme pH values. Carbohydrates, lipids, proteins and nucleic acids these comprise the categories of organic compounds found in living things. These are also called macromolecules, and they are polymers built from individual monomers. It may help to remember that, generally, particles store energy when they are bonded together and release energy when they are split apart. Can you identify the active site on this diagram? What is the purpose of an enzyme? What are some factors that affect the rate of enzyme activity? What does it mean for an enzyme to be denatured? Chapter 7: Cells (p.168-198) Cell Theory Cell Types Cell Organelles Diffusion/Osmosis/Active Transport The next unit explores properties of cells, the smallest units of life. It is important to know the structures that all cells, both eukaryotic and prokaryotic, have in common; they are simply a cell membrane, cytoplasm and DNA. Eukaryotic cells are complex relative to prokaryotic cells. A key difference is that eukaryotic cells contain internal organelles, such as nuclei, mitochondria and ribosomes. Search your notes and textbook pages 173-83 to review the organelles and understand their comparison to a factory. Cell membrane also known as the phospholipid bilayer and it is a particularly important component of cells. Among other duties, it regulates entry to and exit from the cell. This movement across the membrane occurs in one of a few ways, namely, diffusion, osmosis and active transport. Diffusion generally refers to the movement of materials from areas of high concentration to low concentration. Osmosis is the diffusion of water and active transport is an energyintensive process that moves materials from low to high concentration, against the concentration gradient. What other types of movement across the cell membrane exist? Because cell membranes are semi-permeable and allow some materials to pass through while others cannot, the environment outside of the membrane is important. Cells behave differently in isotonic, hypertonic and hypotonic solutions. The relative concentrations of water inside and outside of a cell will affect how much water enters or leaves. Photosynthesis this concept is critically important in biology. All energy on Earth comes from its sun. However, we’re unable to harvest that light energy beaming down. Thankfully, plants are able to do so via photosynthesis. Understanding the pioneering Chapter 8: Photosynthesis (p. 200-218) contributions of the scientists Priestley, Ingenhousz and van Helmont Photosynthesis Pioneers will help you understand the photosynthetic process. Energized Molecules Photosynthesis takes place in the chloroplasts of plant cells. Plants are able to absorb light energy thanks to the pigment chlorophyll. Absorbed light excites electrons which bond to electron carriers to create Light & Pigments Light-Dependent Reactions Calvin Cycle Leaf Stomata energized molecules such as ATP and NADPH. These molecules are crucial to the two-step photosynthetic process, including light-dependent reactions and the Calvin cycle. Chapter 9: Cellular Respiration (p.220-232) Glycolysis Krebs Cycle Electron Transport Chain Fermentation Cellular respiration the aerobic process of breaking down food (glucose) and converting it to usable energy (ATP). Identify and describe the pathways for the conversion of glucose with oxygen (cellular respiration) and without oxygen (glycolysis and fermentation). Questions (Please answer on a separate sheet) Chapter 1: 1. How does an observation differ from an inference? 2. How can a graph of data be more informative than a table of the same data? 3. List and describe each of the biological levels of organization. 4. A young animal normally grows larger no matter what food it eats. Suggest an experiment that would show whether one type of food was better than another at helping an animal to grow faster. Chapter 2: 5. Explain the properties of cohesion and adhesion. Give an example of each property. 6. What are ions and isotopes? 7. Describe two factors that influence enzyme activity and explain how this occurs 8. As part of the digestive process, the human stomach produces hydrochloric acid, HCl. Sometimes excess acid causes discomfort. In such a case, a person might take an antacid such as magnesium hydroxide, Mg(OH)2. Explain how this substance can reduce the amount of acid in the stomach. Chapter 7: 9. Describe the structure of a cell membrane. How does the cell membrane affect the contents of the cell? 10. What is meant by an isotonic solution? Do particles move across the membrane in an isotonic solution? 11. Which organelle helps prevent damage to cells that are subjected to high osmotic pressure? 12. What would happen to a sample of your red blood cells if they were placed into a hypotonic solution? Explain your solution. Chapter 8: 13. Compare the amounts of energy stored by ATP and by glucose. Which compound is used by the cell as an immediate source of energy? 14. How were Priestley’s and Ingenhousz’ discoveries about photosynthesis related? 15. What are leaf stomata and what purpose do they serve for plants? 16. Write a metaphor to explain the energy differences between ADP & ATP and also NADP + and NADPH. 17. EXPLAIN HOW THE LIGHT DEPENDENT REACTION AND CALVIN CYCLE ARE RELATED. Chapter 9: 18. How is glucose changed during glycolysis? What products are produced as a result of glycolysis? 19. What are the two pathways that might follow glycolysis? What factor determines which of the pathways a cell might follow? 20. What is the chemical equation for cellular respiration? 21. Certain types of bacteria thrive in conditions that lack oxygen. What does that fact indicate about the way they obtain energy?