Download Gene Section CENPW (centromere protein W) Atlas of Genetics and Cytogenetics

Atlas of Genetics and Cytogenetics
in Oncology and Haematology
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Gene Section
Short Communication
CENPW (centromere protein W)
Seyoung Jeon, Soojin Lee
Department of Microbiology and Molecular Biology, Chungnam National Institute, 305-764, Daejeon,
Republic of Korea (SJ, SL)
Published in Atlas Database: June 2013
Online updated version : http://AtlasGeneticsOncology.org/Genes/CENPWID51452ch6q22.html
DOI: 10.4267/2042/52069
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence.
© 2014 Atlas of Genetics and Cytogenetics in Oncology and Haematology
Abstract:
Short communication on CENPW, with data on DNA/RNA, on the protein encoded and where the gene
is implicated.
It contains a typical NLS sequence and histone fold
region which is critical for binding to centromeric
DNA.
Identity
Other names: C6orf173, CUG2
HGNC (Hugo): CENPW
Location: 6q22.32
Expression
Widely expressed; kidney, colon, breast, liver, lung,
ovary, prostate, thyroid, etc.
DNA/RNA
Localisation
Description
Exons: 3, coding exons: 3, introns: 2.
Intracellular, nucleus, centromere, kinetochore, nucleus
matrix, nucleolus.
Transcription
Function
Transcript length: 750 bps, open reading frame: 264
bps.
CENP-W was originally identified as a cancerupregulated gene 2 (CUG2) which is commonly
overexpressed in various human cancer tissues.
Although it has high oncogenic activities, CENP-W
also induces cell apoptosis when overexpressed in
certain cell lines. After it was revealed that CENP-W
forms a stable heterodimer with CENP-T and is
localized in kinetochores during mitosis, CENP-W
become recognized as a new member of the inner
centromere protein complex. Subsequent studies have
also shown that CENP-T-W-S-X forms a unique
centromeric nucleosome-like heterotetramer structure
which binds to and supercoils DNA.
Pseudogene
No pseudogene.
Protein
Description
CENP-W gene encodes 88-amino acid protein which
shows ~27% of amino acid sequence similarity to a
general transcription repressor, human DR1.
Figure 1. A genomic structure of the CENPW gene.
Atlas Genet Cytogenet Oncol Haematol. 2014; 18(1)
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CENPW (centromere protein W)
Jeon S, Lee S
Figure 2. Schematic representation of CENPW protein. Each domain is represented with the unique color and name. One nuclear
localization signal (NLS) sequence of 17 amino acids starts at amino acid 13.
Figure 3. mRNA expression of CUG2 in various tumors. DNA chip microarray profile in tumors (closed bar) and normal (open bar)
tissues from the Gene Express Oncology DatasuiteTM of Gene Logic Inc.
As a nucleolus-associated protein, CENP-W may play
critical role in the formation of functional kinetochore,
possibly by facilitating the recruitment of other
centromeric components during interphase, which is
required for proper chromosome segregation during
mitosis.
Implicated in
Various human cancers
Oncogenesis
CENP-W has been found to be upregulated in several
tumor tissues. When the expression level of CENP-W
was examined by DNA chip microarray and RT-PCR
in tumors, six tissues (ovary, liver, lung, pancreas,
colon, and stomach) showed high-fold increases in
expression profiles. Also, CENP-W-transformed
NIH3T3 cells exhibited exceptionally prominent
cancerous phenotypes in vitro as well as in vivo tumor
forming assays.
Homology
According to NCBI-HomoloGene:
Pan troglodytes (chimpanzee): centromere protein W
(NP_001012525.1, 88 aa)
Macacamulatta (Rhesus monkey): centromere protein
W (XP_001107034.2, 88 aa)
Canis lupus familiaris (dog): centromere protein W-like
(XP_003638832.1, 88 aa)
Bostaurus
(cattle):
centromere
protein
W
(NP_001104731.1, 88 aa)
Mus musculus (house mouse): centromere protein W
(NP_001103217.1, 86 aa)
Rattus norvegicus (Norway rat): centromere protein W
(XP_001070657.1, 86 aa).
References
Lee S, Gang J, Jeon SB, Choo SH, Lee B, Kim YG, Lee YS,
Jung J, Song SY, Koh SS. Molecular cloning and functional
analysis of a novel oncogene, cancer-upregulated gene 2
(CUG2). Biochem Biophys Res Commun. 2007 Aug
31;360(3):633-9
Kim H, Lee M, Lee S, Park B, Koh W, Lee DJ, Lim DS, Lee S.
Cancer-upregulated gene 2 (CUG2), a new component of
centromere complex, is required for kinetochore function. Mol
Cells. 2009 Jun 30;27(6):697-701
Mutations
Note
Over expressed in various cancer tissues; notably high
in the ovary (6.3-fold), liver (6.0), lung (4.9), and
pancreas (3.8).
Atlas Genet Cytogenet Oncol Haematol. 2014; 18(1)
Kim H, Lee S, Park B, Che L, Lee S. Sp1 and Sp3 mediate
basal and serum-induced expression of human CENP-W. Mol
Biol Rep. 2010 Oct;37(7):3593-600
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CENPW (centromere protein W)
Jeon S, Lee S
Lee S, Koh W, Kim HT, Kim CH, Lee S. Cancer-upregulated
gene 2 (CUG2) overexpression induces apoptosis in SKOV-3
cells. Cell Biochem Funct. 2010 Aug;28(6):461-8
CNS development in zebrafish. BMC Dev Biol. 2011 Aug
15;11:49
Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V,
Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan KF.
Premitotic assembly of human CENPs -T and -W switches
centromeric chromatin to a mitotic state. PLoS Biol. 2011
Jun;9(6):e1001082
Park EH, Park EH, Cho IR, Srisuttee R, Min HJ, Oh MJ, Jeong
YJ, Jhun BH, Johnston RN, Lee S, Koh SS, Chung YH. CUG2,
a novel oncogene confers reoviral replication through Ras and
p38 signaling pathway. Cancer Gene Ther. 2010
May;17(5):307-14
Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T,
Oyama T, Morikawa K, Cheeseman IM, Fukagawa T. CENP-TW-S-X forms a unique centromeric chromatin structure with a
histone-like fold. Cell. 2012 Feb 3;148(3):487-501
Chun Y, Park B, Koh W, Lee S, Cheon Y, Kim R, Che L, Lee
S. New centromeric component CENP-W is an RNAassociated nuclear matrix protein that interacts with
nucleophosmin/B23 protein. J Biol Chem. 2011 Dec
9;286(49):42758-69
This article should be referenced as such:
Kim HT, So JH, Jung SH, Ahn DG, Koh W, Kim NS, Kim SH,
Lee S, Kim CH. Cug2 is essential for normal mitotic control and
Atlas Genet Cytogenet Oncol Haematol. 2014; 18(1)
Jeon S, Lee S. CENPW (centromere protein W). Atlas Genet
Cytogenet Oncol Haematol. 2014; 18(1):5-7.
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