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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2013
BT 2825 - ENZYMOLOGY & ENZYME TECHNOLOGY
Date : 02/05/2013
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART – A
(20 marks)
Answer all the questions
1. Choose the correct answer:
(5 x 1 = 5 marks)
(1) The energy required to reach the transition state is called as:
(a) Transition energy (b) Activation energy (c) Free energy (d) Reaction energy
(2) Which of the following are the catalytic residues of lysozyme?
(a) Gln35, Ser194
(b) Glu35, Asp52
(c) His 57, Ser102
(d) Glu55, Asp102
(3) Lignin-degrading
degrading enzymes can be best isolated from microbes growing on:
(a) Soil
(b) Fruits
(c) Wood rot
(d) Bread
(4) Recombinant
nant algalsidase alpha is used to treat:
(a) Immunodeficiency (b) Fabry’s disease
(c) Gaucher’s disease
(d) Cystic fibrosis
(5) Tamoxifen is metabolized to endoxifen by the enzyme
(a) CYP2D6
(b) CYP3D6
(c) CYP4D6
(d) CYP5D6
II. State whether the following are true or false, if false, give reason (5 x 1= 5 marks)
(6) The amino acids at the active site of the enzyme do not interact with the substrate.
(7) The lactate dehydrogenase isozyme LDH-1
LDH is present in muscles.
(8) To reduce the number of free sulphydryl groups in an enzyme, the cysteine codons can be replaced
with proline residues.
(9) Lipases are used to give characteristic flavours to cheeses.
(10) Tay Sachs disease is caused by insufficient
insuffi
activity of hexosaminidase A.
III. Complete the following:
(5 x 1 = 5 marks)
(11) Enzyme regulation by phosphorylation is known as ________ modification.
(12) ________ is the number
ber of moles of substrate converted to product per unit time.
(13) ________ cloning is used to screen for microbial enzyme genes.
(14) An enzyme supplement used in the treatment of jelly fish and insect intoxications is ________ .
(15) The marker enzyme for the mitochondrial inner membrane is ________.
IV. Answer the following,each within 50 words only
(5 x 1 = 5marks)
(16) What is the prosthetic group of an enzyme?
(17) Why can natural RNA act as enzymes whereas DNA cannot?
can
(18) Why does the replacement of some asparagine residues increase enzyme thermostability?
(19) What is the application of alkaline phosphatase in genetic engineering?
(20) How is ‘personalized’ medicine related to drug metabolizing enzymes?
PART – B
Answer the following, each in about 500 words only. Draw diagrams
wherever necessary.
(5×8 = 40 marks)
21(a) Explain the thermodynamics of an enzyme reaction.
OR
(b) Discuss the substrate specificity of enzymes and the different ways in which substrate can be
affected when at the active site.
22(a) Describe zymogens with suitable examples.
OR
(b) Write notes on:
(i) Catalytic mechanism of tryptophan synthase
(ii) Substrate binding by chymotrypsin and trypsin
23(a) Discuss the use of in vitro recombination to obtain novel enzyme genes.
OR
(b) Explain how the thermostability of an enzyme can be improved by addition of disulphide bonds.
24(a) Give an account of the enzymes used in the fruit and vegetable processing industry.
OR
(b) Explain the design and application of an enzyme electrode.
25(a) Write notes on:
(i) Diagnostic enzymes in cancer
(ii) Primary and secondary serum enzymes
OR
(b) Enlist the enzyme markers of xenobiotic toxicity.
PART – C
Answer any TWO of the following, each in about 1500 words; Draw diagrams
wherever necessary.
(2×20 = 40 marks)
(26) Describe the following:
(i) Types of enzyme inhibition
(ii) Ribozymes
(27) Write notes on:
(i) Natural isolate and proteomic screening for enzyme producing microbes
(ii) Increasing tyrosyl –tRNA synthetase activity by protein engineering
(28) Discuss the following:
(i) Enzyme replacement therapy for cancer
(ii) Enzymes used in the alcohol and starch industry
(29) Explain the following:
(i) Glucose-6-phosphate dehydrogenase deficiency
(ii) Enzymes in the diagnosis of hepatic diseases