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Scientific Method 1. STEPS Problem Research Hypothesis Procedure / Data Results / Conclusion 2. Hypothesis Educated guess / a “prediction” based on your research 3. Independent Variable What “I” control, as the experimentor Sometimes called “Manipulated Variable” 4. Dependent Variable • Changes “on its own”, as a RESULT of the experiment • “Responding” Variable • ALWAYS graphed on Yaxis 5. Constants/Controls ►Constants: what keep the SAME ►Control: does NOT receive the I.V. (for COMPARISON) Effect of Salt on Growth Effect of Salt on Growth Growth DV 0 5 IV 10 % SALT 6. Validity/Reliability VALID: measures what it says it measures RELIABLE: can repeat it & get the same results 7. DATA Greatest – Lowest Mean: Average Range: 8. Metrics Mass: Gram Volume: Liter Distance: Meter 9. Water Structure +++ Hydrogen + Bond + -_-- + 10. Water Properties • A POLAR molecule!! A. Form HYDROGEN bonds, making water COHESIVE and ADHESIVE B. Water ABSORBS heat, stabilizing land & sea TEMPERATURES 10. Water Properties C. Evaporates … rids of excess HEAT D. Ice FLOATS, preventing LAKES from freezing SOLID E. Universal SOLVENT (can DISSOLVE many 11. BONDING A. IONIC: + to – (ex: Na+Cl- or salt) B. COVALENT: share ELECTRONS (ex: H2O) C. HYDROGEN: bonds between MOLECULES, such as WATER 12. Water DISSOCIATES into H+ and OH- ions, measured as pH H20 H+ + OH- pH SCALE Neutral 0 14 7 Acidic Basic Neutral = 7 Acidic Ranges = 0 – 6 Basic Ranges = 8 - 14 14. Importance of pH Cells & enzymes only tolerate SMALL CHANGES in pH….. Every cell/enzyme functions best within a narrow RANGE Acidic-loving grows best between: 0 – 6 Neutral-loving best at/near: 7 15. CELL MEMBRANE 15. Cell Membrane Selective Permeability = Lets some things in, not others Help maintain HOMEOSTASIS (term for keeping everything in BALANCE) CELL TRANSPORT DIFFUSION: movement of materials from HIGH conc. To LOW conc. OSMOSIS: WATER (from H L) ACTIVE TRANSPORT: uses ENERGY (can go from L H) 16. ELEMENTS Remember H N the “CHNOPS” !! C P O S 17. ENZYMES ….they serve as CATALYSTS ….they SPEED UP reactions without being CHANGED or USED UP Enzymes are PROTEINS (contain C – N bonds) 17. ENZYMES Active Site: where enzyme attaches to the substrate Substrate: molecule that is changed by the enzyme Product: RESULT of the enzyme-substrate reaction “Lock & Key” = specific enzyme for a specific substrate 17. ENZYMES 18. MACROMOLECULES CARBOHYDRATES LIPIDS PROTEINS NUCLEIC ACIDS CARBS: ELEMENTS C, H, O Sucrose (endings in “ose”) Polysaccharides Carbs: Buiding Blocks Repeating SUGAR units: Carbs: Function/Importance ENERGY (through Respiration – ATP) STRUCTURE (as Cellulose in Plant cell walls) LIPIDS: Elements C, H, O, sometimes P (if in cell membrane Oils Waxes Lipids: Building Blocks Glycerol + Fatty Acids: CH2 LIPIDS: Function/Importance CELL MEMBRANES! Long-term energy storage Insulation Proteins: Elements • • • • C, H, O, and N Enzymes Muscles Cell Structure Proteins: Building Blocks Built of Repeating AMINO ACID units Also called POLYPEPTIDES Proteins: Function/Importance • Structural foundation of ALL CELLS • ENZYMES – act as catalysts Nucleic Acids: Elements C, H, O, N, P DNA RNA Nucleic Acids: Buidling Blocks NUCLEOTIDES: SUGAR + BASE + Phosphate Nucleic Acids: Functions Store genetic information (DNA) Carry instructions to the ribosomes (mRNA) 20. MICROSCOPES A. Dissecting B. Compound Light C. Electron Scanning TOTAL MAGNIFICATION = Obj. Lense X Eyepiece 21. CELL THEORY A. All living things are made of cells B. Cells = basic unit of structure and function C. Cells come from preexisting cells 22. Types of Cells Prokaryote (no nucleus) Eukaryote (have nucleus & organelles) EUKARYOTE Cells Nucleus Mitochondria Chloroplasts Golgi, E.R. Vacuoles, etc. 22. BOTH Cell Types Have: DNA / RNA Cell Membrane Cytoplasm Ribosomes Cytoskeleton PROKARYOTE Cells: NO Nucleus NO Organelles Bacteria Kingdoms Cell Organelles NUCLEUS: control center – DNA, chromosomes, genes CELL MEMBRANE: surrounds cell; bilipid layer; controls in & out CYTOPLASM: area between cell membrane & nucleus CELL ORGANELLES • GOLGI APPARATUS: finishing/packaging of molecules • CHLOROPLASTS: site of photosynthesis (green) • VACUOLES: store/transport wastes, water, or food CELL ORGANELLES RIBOSOMES: site of PROTEIN SYNTHESIS!!! MITOCHONDRIA: powerhous of cell; site of respiration (ATP) ENDOPLASMIC RETICULUM (ER): transports; “rough” has ribosomes CELL ORGANELLES CENTRIOLES: assist in cell division LYSOSOMES: digestive enzymes (“suicide sacs”) NUCLEOLUS: in nucleus; produces RNA 6 Kingdoms ARCHAEBACTERIA: P,U,A,H EXAMPLES: Halophiles; hot sulphur springs bacteria CHARACTERISTICS: live in EXTREME environments; most primitive bacteria 29. CLASSIFICATION LINNAEUS = scientist Binomial Nomenclature: Genus (capitalized) + Species Example: Homo sapiens (Genus species) 30. Phylogenic Trees Show EVOLUTIONARY relationships (closer together = closer their relationship) 6 KINGDOMS Abbreviations Key (bottom): P Prokaryote E Eukaryote U Unicellular M Multicellular A Autotroph H Heterotroph EUBACTERIA P, U, A, H Examples: E. Coli (intestines) Acidophilis (yogurt) Streptococcus Characteristics: common, true bacteria; diseases; important as decomposers PROTISTA E, U or M, A or H Examples: Amoeba, Paramecium, Plankton Characteristics: key producers (aquatic) & basis of food chain; both plant & animal “like” FUNGI E, M (mostly) or U, H Examples: mushrooms, molds, yeast Characteristics: absorb food; spore-producers; decomposers PLANTS E, all M, all A Examples: mosses, ferns, cone-trees, flowering plants Characteristics: producers in all biomes; food, O2, homes, maintain environment ANIMALS E, all M, all H Examples: sponge, jellyfish, frog, bird, mammal Characteristics: decomposers, scavengers, consumers, predators, commercially imp. VIRUSES vs. CELLS BOTH have DNA/RNA Virus NOT made of cells, needs a HOST to reproduce ENERGY PYRAMIDS Tropic levels ECOLOGY: Terms POPULATION: all members of a single species in same area COMMUNITY: several different species together ECOSYSTEM: community + all abiotic factors ECOLOGY: Terms • BIOSPHERE: all part of Earth capable of supporting life • BIOTIC FACTORS: the LIVING parts of an environment • ABIOTIC FACTORS: the NONLIVING parts (dirt, rocks) ECOLOGY: Terms • NORMAL GROWTH: “S” shaped curve; levels off at carrying capacity • EXPONENTIAL GROWTH: “J” shaped; increasing rapidly ECOLOGY: Terms LIMITING FACTOR: what is in “shortest supply” CARRYING CAPACITY: maximum population size an environment can support ECOLOGY: Terms FOOD CHAIN: grass cow human FOOD WEB: show ALL possible feeding relationships ECOLOGY: Terms SYMBIOSIS: close relationship between 2 different species MUTUALISM: + (gain) & + (gain) COMMENSALISM: + & “neither” PARASITISM: + and – (harmed) ECOLOGY: Terms • SUCCESSION: orderly replacement of species (going from “field” to “forest”) • CLIMAX COMMUNITY: LAST stage of succession; stable, mature 19. Photosynthesis: Inputs Chlorophyll + Sun + CO2 + Water 19. Respiration: inputs SUGAR + O2 + Enzymes 19. Products (Photosyn.) SUGARS OXYGEN 19. Products: Respiration ATP CO2 H2O (energy) SITE: Photosynthesis CHLOROPLAST SITE: Respiration MITOCHONDRIA RATE: Photosynthesis Amounts of: Light CO2 Water Temperature RATE: Respiration Amounts of: Sugar Oxygen Enzymes 19.A “Light Reaction” or… Photochemical Reaction = H2O + SUN H+ + O2 B. “Dark Reaction” or … Carbon Fixation = H+ + CO2 SUGARS 19C. RESPIRATION: can be either AEROBIC (with oxygen) or ANAEROBIC (without oxygen) -- look for LACTIC ACID as a product GENETICS TERMS DOMINANT: the trait that SHOWS, when present (“A”) RECESSIVE: hidden trait (unless homozygous) (“a”) CHROMOSOME: threads of genetic material (genes) GENETICS TERMS ALLELE: one gene form (can be dominant or recessive) HOMOZYGOUS: gene forms are the SAME (“AA”) HETEROZYGOUS: gene forms are DIFFERENT (“Aa”) GENTICS TERMS GENOTYPE: the gene forms an organism has PHENOTYPE: the way an organism LOOKS PUREBRED: same as “Homozygous” (same) GENETICS TERMS HYBRID: same as “heterozygous” (different) KARYOTYPE: charted arrangement of chromosomes MENDEL: founder of Genetics CODOMINANCE: BOTH forms are expressed (“roan”) GENETICS TERMS INCOMPLETE DOMINANCE: intermediate form is expressed (“red” + “white” = pink) HAPLOID: half-set of chrom. (sex cells) DIPLOID: double-set of chrom. (all other cells) GENETICS TERMS CLONING: make an identical copy from a single cell GAMETE: sex cell (haploid) SOMATIC CELL: all BODY cells (other than sex cells) 24. Punnett Squares B B BB b Bb b Bb bb 25. STAGES of Cell Division A. MITOSIS: 2 identical daughter cells (each 2n) 25. Cell Division: MEIOSIS B. MEIOSIS: 4 haploid sex cells 26. Mitosis V. Meiosis Mitosis Meiosis Cell division ALL cells IPMAT once 2 diploid cells 2n 2n + 2n Cell division Sex cells only IPMAT twice 4 haploid cell 2n n+n+n+n REPRODUCTION • Sexual: union of male & female gametes • Asexual: producing a copy WITHOUT forming gametes 26. DNA Structure/Function Double-helix …chains of NUCLEOTIDES, … SUGAR + BASE + PHOSPHATE …carry the genetic BLUEPRINTS 26. DNA vs. RNA DNA Double-strand Deoxyribose (sugar) A-T, C-G One form: DNA Cell’s master code RNA Single strand Ribose (sugar) A-U, C-G mRNA, tRNA, rRNA Protein synth. 27. Protein Synthesis Transcription 27. Protein Synthesis Translation: 28. EVOLUTION Key scientist: Darwin A. FOSSIL: earlier, common ancestors (similar to modern species) 28. Evolution B. EMBRYOLOGY: (e.g. vertebrates) all look nearly the same at first ….COMMON ANCESTORY! 28. Evolution C. AMINO ACID SEQUENCES: fewer differences = more closely related 28. Evolution D. SIMILAR STRUCTURES: Analogous: Bird/Bat wing Homologous: Vert. forelimb Vestigial: Appendix 28. Evolution NATURAL SELECTION: organism BEST SUITED to environment survives & leaves more offspring “Survival of the Fittest!” 31. FOSSIL Dating (see board) Which levels are oldest? Newest? What does this tell us about prior habitats? THEORIES of EVOLUTION • GRADUALISM: species evolved gradually over long periods of time • PUNCTUATED EQUILIBRIUM: periods of RAPID change, followed by little or no change 34. CHESAPEAKE BAY … largest ESTUARY in US (where RIVERS meet OCEAN) A. … reduced forest cover, chemicals released into Bay, runoff, intensive farming, loss of bay GRASSES 34. CHESAPEAKE BAY B. …filters / cleans & provides food & nesting cover C. …LESS salinity as you go from Ocean to Bay to marsh to rivers to creeks PLANTS Nonvascular = NO ROOT/STEM for transporting WATER & NUTRIENTS (ex: Moss, bryophytes) Vascular: … XYLEM (water), PHLOEM (sugars): ferns, gymnosperms, angiosperms MONOCOTS DICOTS LEAF 1.CUTICLE 2.UPPER EPIDERMIS 3.PALISADE LAYER 4.SPONGY LAYER VEIN: 5.XYLEM 6.PHLOEM 8.GUARD CELLS 9.STOMATA 7.LOWER EPIDERMIS FLOWER INVERTEBRATE Animals NO BACKBONE SHELLS, Exoskeletons, … SOFT-BODIED Sponge, Jellyfish, Worms, Molluscs, Insects VERTEBRATE Animals ENDOSKELETON & BACKBONE LARGER, MORE MOBILE Fish, Amphibians, Reptiles, Birds, Mammals ALL Animals HETEROTROPHS MULTICELLULAR INGEST their FOOD